| 1
| Gunn, Khalil | JR | Gadsden County | 10.97 | Wakulla Quad Meet 2 | Feb 25, 2021 | |
| 2
| Roberts, Denvantae | SR | Gadsden County | 11.39 | Wakulla Quad Meet 3 | Mar 4, 2021 | |
| 3
| Riley, Zaire | JR | Gadsden County | 11.45 | Wakulla Quad Meet 2 | Feb 25, 2021 | |
| 4
| Wallace, Jeremy | JR | Suwannee | 11.49 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.7 |
| 5
| Atkinson, Terrell | JR | Suwannee | 11.53 | SFHS Mini Meet #1 | Feb 18, 2021 | 1.5 |
| 6
| Graham, Malachi | JR | Suwannee | 11.59 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.8 |
| 7
| Moore, Jaquez | SR | Suwannee | 11.60 | Ron Riddle Invitational | Feb 26, 2021 | |
| 8
| Thomas, Johntarious | JR | Gadsden County | 11.61 | Wakulla Quad Meet 2 | Feb 25, 2021 | |
| 9
| Smith, Javarion | SO | Suwannee | 11.64 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.8 |
| 10 | Coleman, DJ | SO | Suwannee | 11.70 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.6 |
| 11 | Jackson, Dematres | SR | Gadsden County | 11.72 | Wakulla Quad Meet 3 | Mar 4, 2021 | |
| 12 | Jones, Chaddrick | SO | Gadsden County | 11.74 | Wakulla Quad Meet 3 | Mar 4, 2021 | |
| 13 | Mason, Ethan | SR | Gadsden County | 11.82 | Wakulla Quad Meet 3 | Mar 4, 2021 | |
| 14 | Owens, Marquavious | FR | Suwannee | 11.83 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.6 |
| 15 | Kelly, Ethan | SR | Marianna | 11.91 | Bozeman Host Meet | Mar 4, 2021 | |
| 16 | Flowers, Camden | JR | Taylor County | 11.99 | Wakulla Quad Meet 1 | Feb 16, 2021 | |
| 17 | Williams, Stylez | JR | Taylor County | 12.02 | Wakulla Quad Meet 2 | Feb 25, 2021 | |
| 18 | Fuery, Brian | SR | Suwannee | 12.04 | SFHS Mini Meet #1 | Feb 18, 2021 | 1.5 |
| 19 | Kirksey, Alvin | SR | Godby | 12.09 | Chiles Relays | Feb 20, 2021 | 0.9 |
| 20 | Tucker, Kenan | SR | Suwannee | 12.12 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.8 |
| 21 | Brown, Demontay | FR | Suwannee | 12.13 | SFHS Mini Meet #1 | Feb 18, 2021 | 1.1 |
| 22 | Milton, Trevion | SO | Suwannee | 12.20 | SFHS Mini Meet #1 | Feb 18, 2021 | 1.8 |
| 23 | Samano, Aiden | JR | Taylor County | 12.24 | Wakulla Quad Meet 2 | Feb 25, 2021 | |
| 24 | Beach, Garrison | JR | Suwannee | 12.28 | SFHS Mini Meet #1 | Feb 18, 2021 | 1.5 |
| 25 | Morgan, Parker | SO | Chipley | 12.31 | Bozeman Host Meet | Mar 4, 2021 | |
| 26 | Bell, Termain | JR | Marianna | 12.32 | Bozeman Host Meet | Mar 4, 2021 | |
| 26 | Manny, Quendarius | SO | Gadsden County | 12.32 | Wakulla Quad Meet 3 | Mar 4, 2021 | |
| 28 | McCreary, Stavon | JR | Madison County | 12.38 | Wakulla Quad Meet 1 | Feb 16, 2021 | |
| 29 | Pratt, Brandon | SR | Suwannee | 12.43 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.8 |
| 30 | Jackson, Ja'ohn | SO | Madison County | 12.54 | Wakulla Quad Meet 1 | Feb 16, 2021 | |
| 31 | Wainwright, Will | FR | Suwannee | 12.55 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.8 |
| 32 | Wainwright, Sam | FR | Suwannee | 12.57 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.8 |
| 33 | Robinson, Brandon | SO | Suwannee | 12.58 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.9 |
| 34 | Sims, TaShaun | FR | Chipley | 12.69 | Bozeman Host Meet | Mar 4, 2021 | |
| 35 | Harvey, Tre'kevion | SO | Marianna | 12.71 | Bozeman Host Meet | Mar 4, 2021 | |
| 36 | Hackett, Trent | SR | Florida High | 12.72 | Jesse Forbes HS Invitational | Feb 27, 2021 | |
| 36 | Simmons, Charles | JR | Taylor County | 12.72 | Wakulla Quad Meet 1 | Feb 16, 2021 | |
| 38 | McGee, Derrick | FR | Florida High | 12.73 | Jesse Forbes HS Invitational | Feb 27, 2021 | |
| 39 | McCovery, Da'Quan | FR | Chipley | 12.79 | Bozeman Host Meet | Mar 4, 2021 | |
| 40 | Gilespie, Chase | SR | Godby | 12.91 | Jesse Forbes HS Invitational | Feb 27, 2021 | |
| 41 | Brown, Andrew | JR | Suwannee | 13.00 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.9 |
| 42 | Ivey, Ka'Darien | SO | Suwannee | 13.06 | SFHS Mini Meet #1 | Feb 18, 2021 | 1.1 |
| 43 | Lang, Kodi | FR | Suwannee | 13.21 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.8 |
| 43 | Pate, Charles | JR | Suwannee | 13.21 | SFHS Mini Meet #1 | Feb 18, 2021 | 1.1 |
| 45 | Gentle, Omarrion | FR | Madison County | 13.22 | Wakulla Quad Meet 1 | Feb 16, 2021 | |
| 46 | Smith, Jamarion | FR | Suwannee | 13.52 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.9 |
| 47 | Dyer, Jacob | FR | Suwannee | 13.53 | SFHS Mini Meet #1 | Feb 18, 2021 | 0.9 |
| 48 | Gross, Landarren | FR | Suwannee | 14.10 | SFHS Mini Meet #1 | Feb 18, 2021 | 1.6 |
| 49 | Ellis, Chadrick | 7
| Taylor County | 14.74 | Chiefland Home Meet 2 | Mar 3, 2021 | |
| 50 | Griffin, Montray | 8
| Taylor County | 14.77 | Chiefland Home Meet 2 | Mar 3, 2021 | |
| 51 | Upshaw, Camen | 7
| Taylor County | 15.29 | Wakulla Quad Meet 1 | Feb 16, 2021 | |
