Girls' 4 x 100 Relay
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| PL | TEAM | ATHLETES | TIME | TIME | TIME | SC |
|---|---|---|---|---|---|---|
| 1 | Rockledge | Tykira Glasco, Shamya Joiner, Shadae Hawkins, Jaida Williams-Goins | 52.56 | 48.22 | 40.99 | 10 |
| 2 | Seminole (Sanford) | Oludoyin Soremi, Oluwadara Soremi, Keyara Hillery, Sidney Allen | 47.60 | 49.07 | 1:01.83 | 8 |
| 3 | Lake Howell | Mikiya Lowe, TaNyiah Golson, Makayla Watson, Ava Stokes | 57.64 | 50.56 | 49.55 | 6 |
| 4 | Oviedo | Samantha Maiorino, Eliana Nemasango, Alexandra Vecchiarelli, Ayden Chandler | 1:06.51 | 54.96 | 51.12 | 5 |
| 5 | Lake Mary | Keniya Bullard, Kendra Muhammad, Ashanti Bryant, Elisa Wood | 52.34 | 56.89 | 1:02.58 | 4 |
| 6 | City of Life Christian | Jennaya Ortiz, Jazzmin Puckett, Valerie Sical, Anthea Alley | 1:14.94 | 58.54 | 1:10.25 | 3 |
| 7 | Geneva School | Aurora Flores, Anna Hope Fretwell, Bailey White, Loralei Flores | 53.90 | 59.88 | 53.90 | 2 |
| Harmony | Jirehlis Chiclanan, Drea Thompson, Morgan Pascual, Adriana Rosa | DQ | DQ | DQ |