| 1. | 2. | Olympia | Apopka | Jade Blake, Lauren Guthrie, Alyia Green, Hannah Douglas | Amaya Phillips, Jetzymar Rivera, Dezha Walker, Alliann Johnson | 47:04:01.4231 | 53:04:29.067 | 8710 | 17452 |
| 2. | 12. | Osceola (Kissimmee) | Windermere | Amaya Phillips, Jetzymar Rivera, Dezha Walker, Alliann Johnson | Alyssa Mark, Yvanna Bollange, Jessica Doreste, Shyanne Mayes | 4:03.5577 | 1:04:28.7615 | 80 | - |
| 3. | 13. | Apopka | Osceola (Kissimmee) | Kalaila Smith, Astoria Beckett, Hannah Jones, Samantha Andreetti | Glorianna Echevarria, Arianna White, Zakhia Henderson, Kutia Sinclair | 4:04.54 | DNS | 6 | 80 |
| 4. | 11. | West Orange | Olympia | Alyssa Mark, Yvanna Bollange, Jessica Doreste, Shyanne Mayes | Carolyn Brown, Michelle Gunn, Reathasia Outler, Daniella Paolicelli | 4:11.7966 | 4:04.54 | 586 | 80 |
| 5. | 9. | Windermere | Edgewater | Adeena Ademu John, Sheintah Artison, Nosharai Harris, Layla Huntley | Alyssa Mark, Yvanna Bollange, Jessica Doreste, Shyanne Mayes | 2:04:25.9416 | 33:05:17.75 | 17452 | - |
| 6. | 14. | Lake Brantley | Lake Brantley | Amilyah Robinson, Anya Spragg, Cadeeja Pennant, Jordan Stevens | Alba Rivas, Meya Felix, Shayla Jones, Jazmine Scott | 1:04:28.7615 | 4:03.5577 | 31 | - |
| 7. | 4. | Ocoee | Bartow | Eriona Sherwood, Kaylah Ward, Achante Daniels, Ariana Rivers | Adeena Ademu John, Sheintah Artison, Nosharai Harris, Layla Huntley | 53:04:29.067 | 53:04:29.067 | 0237 | 6 |
| 8. | 12. | Oak Ridge | East Ridge | Bensdjadini Michel , Naika Metellus, Falonne Michel , Paquisha Eutsey | Adeena Ademu John, Sheintah Artison, Nosharai Harris, Layla Huntley | 17:04:30.7710 | 4:11.7966 | 13 | 13 |
| 9. | 1. | Boone | Ocoee | Carolyn Brown, Michelle Gunn, Reathasia Outler, Daniella Paolicelli | Marina Brown, Sagan Simmons, Gabrielle Brice, Breniea Smith | 4:34.9879 | 4:36.983 | - | - |
| 10. | 9. | University (Orlando) | South Lake | Linette Fernandez, Stephanie Colon, TreazuÃÂÃÂre Sims, Dejah Ojeda | Amilyah Robinson, Anya Spragg, Cadeeja Pennant, Jordan Stevens | 4:35.999 | 17:04:30.7710 | - | 6 |
| 11. | 7. | Edgewater | Windermere | Marina Brown, Sagan Simmons, Gabrielle Brice, Breniea Smith | Trinity Fuery, Hailey Colon, Tyshonne Tollie, Jalia Harris | 4:36.983 | 4:38.5961 | - | 31 |
| 12. | 3. | Bartow | Oak Ridge | Trinity Fuery, Hailey Colon, Tyshonne Tollie, Jalia Harris | Amilyah Robinson, Anya Spragg, Cadeeja Pennant, Jordan Stevens | 4:38.5961 | 53:04:29.067 | - | - |
| 13. | | East Ridge | Bartow | Glorianna Echevarria, Arianna White, Zakhia Henderson, Kutia Sinclair | Jade Blake, Lauren Guthrie, Alyia Green, Hannah Douglas | 4:49.6358 | 53:04:29.067 | - | 31 |
| 14. | 13. | South Lake | Bartow | Alba Rivas, Meya Felix, Shayla Jones, Jazmine Scott | Bensdjadini Michel , Naika Metellus, Falonne Michel , Paquisha Eutsey | 33:05:17.75 | 17:04:30.7710 | - | - |
| | 8. | Villages | Oak Ridge | Sydney Richason - Bugg, Rebecca Clark , Brookette Bryant, Ella Kate Daley | Glorianna Echevarria, Arianna White, Zakhia Henderson, Kutia Sinclair | DNS | 1:04:28.7615 | - | 17452 |
