1 | Herrera, Geraldine | SO | Bishop Verot | 24.55 | FHSAA Class 2A/Para-Athlete Track & Field State Championship | May 8, 2021 | -0.7 |
| 2 | Smith, Terrietta | FR | Booker | 25.13 | FHSAA 2A Region 3 | May 1, 2021 | -0.9 |
| 3 | Hecht , Kendall | FR | Parrish | 26.57 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 4
| Sarnac, Ella | SR | Bishop Verot | 26.79 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 5
| Peterson, Jakai | FR | Booker | 27.14 | 48th RAM Invite 2021 | Feb 27, 2021 | -1.4 |
| 6
| Beach, Avery | JR | Cardinal Mooney | 27.35 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 7
| Arrington, Raja | SR | Booker | 27.37 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 8
| Storr, Dominique | SR | Booker | 27.83 | Sarasota County Track and Field Championship Meet | Apr 8, 2021 | |
| 9
| Videnka, Margaret | JR | Cardinal Mooney | 27.87 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 10 | Veitkus, Abigail | SR | Cardinal Mooney | 28.12 | Spring Break Invite at IMG | Mar 20, 2021 | 2.8 |
| 11 | Lamprecht, Gabiella | SO | Parrish | 28.18 | Manatee County Varsity Championship | Apr 8, 2021 | |
| 12 | Koscho, Sally | SO | Cardinal Mooney | 28.52 | Sarasota County Track and Field Championship Meet | Apr 8, 2021 | |
| 13 | Greeman, Madyson | FR | Parrish | 28.70 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 14 | Taglieri, Juliana | SR | Bishop Verot | 28.77 | Charlotte Invitational | Mar 12, 2021 | -0.1 |
| 15 | Ragazzone, Lauren | SR | Lemon Bay | 28.82 | Larry Richardson Memorial Invitational | Mar 5, 2021 | |
| 16 | Dunlap, Quincey | FR | Lemon Bay | 28.86 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 17 | Ash, Ty'Anna | JR | Booker | 28.93 | North Port Invite 2021 | Mar 12, 2021 | -0.2 |
| 18 | Park, Cadence | FR | Bonita Springs | 29.15 | Panther Showcase | Apr 1, 2021 | 3.3 |
| 19 | Cherniak, Sophia | JR | Lemon Bay | 29.23 | Larry Richardson Memorial Invitational | Mar 5, 2021 | |
| 20 | Sebok, Csenge | JR | Booker | 29.39 | Sarasota County Track and Field Championship Meet | Apr 8, 2021 | |
| 21 | Hobson, Darrian (Lexi) | SO | Bonita Springs | 29.45 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 22 | Fischer, Megan | SR | Lemon Bay | 29.49 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 23 | Frantz, Kate | SO | Oasis | 29.96 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 24 | Solas, Emily | 8
| Gateway Charter | 30.18 | Lions Invitational FBA Naples | Apr 9, 2021 | 1.3 |
| 25 | Smallwood, Abigal | JR | LaBelle | 30.45 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 26 | Piotrowski, Addison | FR | Bonita Springs | 30.53 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 27 | Ode, Sophia | SO | Bonita Springs | 30.70 | Panther Showcase | Apr 1, 2021 | 4.7 |
| 28 | Panico, Reagan | FR | Parrish | 30.87 | Manatee County Freshman/ Sophomore Championship | Mar 8, 2021 | |
| 29 | Doan, Emily | SO | Oasis | 31.01 | Ida Baker FD Indoor/Outdoor Invitational | Feb 19, 2021 | 2.9 |
| 30 | Walker, Kendyl | FR | Parrish | 31.02 | Palmetto JV Meet | Feb 22, 2021 | |
| 31 | Sebiro, Chloe | JR | Booker | 31.03 | Palmetto Varsity Invitational | Mar 2, 2021 | |
| 32 | Maier, Jill | FR | Oasis | 31.27 | Panther Showcase | Apr 1, 2021 | 4.7 |
| 33 | Williams, Za'Kiera | SO | Booker | 31.48 | Palmetto Varsity Invitational | Mar 2, 2021 | |
| 34 | Glidden, Madison | SO | Lemon Bay | 31.80 | Sarasota County Track and Field Championship Meet | Apr 8, 2021 | |
| 35 | Hutchinson, Brooke | FR | Lemon Bay | 32.14 | North Port Invite 2021 | Mar 12, 2021 | 0.6 |
| 36 | louis, phoebe | JR | Gateway Charter | 32.30 | First Baptist Academy (Naples) Invitational | Mar 11, 2021 | -0.2 |
| 37 | Simpson, Jasmine | | Bishop Verot | 32.75 | Viking Elite Classic 2021 | Feb 26, 2021 | 0.3 |
| 38 | Clay, Samantha | SO | Oasis | 33.01 | Panther Showcase | Apr 1, 2021 | 3.7 |
| 39 | Via-Reque, Eliana | SR | Cardinal Mooney | 33.36 | SSES Quad Meet 1 | Feb 19, 2021 | |
| 40 | Hutchinson, Brooke | FR | Lemon Bay | 33.39 | 48th RAM Invite 2021 | Feb 27, 2021 | -2.1 |
| 41 | Bach, Kate | FR | Bonita Springs | 33.44 | Ida Baker FD Indoor/Outdoor Invitational | Feb 19, 2021 | 3.4 |
| 42 | Faatz, Samantha | FR | Oasis | 33.60 | Ida Baker FD Indoor/Outdoor Invitational | Feb 19, 2021 | 3.3 |
| 43 | Thomas, Antwanesha | JR | Booker | 33.92 | Palmetto Varsity Invitational | Mar 2, 2021 | |
| 44 | Lee, Leesia | FR | LaBelle | 34.16 | Viking Elite Classic 2021 | Feb 26, 2021 | 0.3 |
| 45 | sanon, samantha | 8
| Gateway Charter | 34.19 | Lions Invitational FBA Naples | Apr 9, 2021 | 3.0 |
| 46 | Rogers, Isabella | SR | Cardinal Mooney | 34.27 | SSES Quad Meet 1 | Feb 19, 2021 | |
| 47 | calvert, brooklyn | JR | Gateway Charter | 34.76 | Lions Invitational FBA Naples | Apr 9, 2021 | 3.0 |
| 48 | Osinski, Emma | SO | Oasis | 34.85 | North Port Invite 2021 | Mar 12, 2021 | 0.9 |
| 49 | Detrinidad, Kiara | SO | Parrish | 35.05 | Palmetto JV Meet | Feb 22, 2021 | |
| 50 | Ziegler, Saniah | JR | Booker | 35.18 | Manatee Hurricanes Invitational | Feb 19, 2021 | |
| 51 | Rosas-Reyes, Valerie | FR | LaBelle | 37.59 | Clewiston Invitational 2021 | Apr 1, 2021 | -0.4 |
| 52 | Woodrow-Frister, Katherine | JR | Oasis | 37.67 | 7th Annual Loretta Purish Invitational-Ladies Only | Mar 4, 2021 | -0.2 |
| 53 | Reagan, Olivia | SR | Bishop Verot | 38.18 | Charlotte Invitational | Mar 12, 2021 | -0.1 |
| 54 | Rosas-Reyes, Valerie | SO | LaBelle | 40.69 | FHSAA 2A District 12 | Apr 15, 2021 | |
| 55 | Fayette, Abigail | SO | Cardinal Mooney | 46.10 | Larry Richardson Memorial Invitational | Mar 5, 2021 | |
| 55 | Love, Annalise | FR | Cardinal Mooney | 46.10 | Larry Richardson Memorial Invitational | Mar 5, 2021 | |
FHSAA 2A