| 1. | 16. | Dunbar | Community School (Naples) | Ryan Livingston, Daniel Ocampo, Ivan Marin-Padilla, Olmar Bartolon | Samuel Shields, Harvey Sarajian, Jackson Parry, Preston Gibson | 8:22.144 | 8:22.79 | 1052 | 95 |
| 2. | 6. | Fort Myers | Community School (Naples) | Joseph Koraya, Colsen Palmer, Quin Roberts, Timmy Schultz | Caeleb Spriggs, Amare Archie, Jesus Us-Tiu, Christopher Singleton | 8:22.79 | 50:10:26.3464 | 585 | - |
| 3. | 14. | Estero | Fort Myers | Kolton Pickard, Max Sedmak, Avery Geerlings, Liam Sedmak | Isaac Magallanes, Brendan Flavin, Christopher Hausler, Andrew Light | 52:08:32.7059 | 4:09:49.837 | 4667 | - |
| 4. | 8. | North Fort Myers | Fort Myers | Mattias Flamholc, Carlos Troche, Josh Ortega, Nekhi Perry | Sergi Guzman, Reece Foland, Ethan Gable, Chase Lupo | 8:44.25 | 8:47.890 | 95 | - |
| 5. | 14. | Evangelical Christian | Fort Myers | Jacob Canup, Porter Crawford, Kelby Piper, Noah Gergley | Evan Barker, Nicholas Del Pizzo, Brandon Pierce, James De Haan | 8:47.890 | 8:44.25 | 347 | - |
| 6. | 15. | Community School (Naples) | Community School (Naples) | Sergi Guzman, Reece Foland, Ethan Gable, Chase Lupo | Caeleb Spriggs, Amare Archie, Jesus Us-Tiu, Christopher Singleton | 8:52.9948 | 4:09:49.837 | 13 | 95 |
| 7. | 10. | South Fort Myers | Cape Coral | Joni Cintron, Baine Dionne, Joel Valentin, Aaron Huber | Evan Barker, Nicholas Del Pizzo, Brandon Pierce, James De Haan | 53:09:11.2825 | 8:22.144 | 62 | - |
| 8. | 3. | Riverdale | Community School (Naples) | Evan Barker, Nicholas Del Pizzo, Brandon Pierce, James De Haan | Sergi Guzman, Reece Foland, Ethan Gable, Chase Lupo | 23:09:16.57 | 4:09:49.837 | 91194 | 347 |
| 9. | 10. | Naples | Island Coast | Samuel Shields, Harvey Sarajian, Jackson Parry, Preston Gibson | Mason Menassa, Ethan Lukow, Braden Valdez, Gaston Salvador | 9:27.76 | 23:09:16.57 | - | 13 |
| 10. | 8. | Canterbury (FM) | Canterbury (FM) | Mason Jessogne, Riley Johnson, Travis Heinrichs, Charles Meagher | Connor Rees, Naseem Abujudeh, Malachi Vann, Ricardo Charite | 9:36.14 | 9:27.76 | - | - |
| 11. | 7. | Cape Coral | Port Charlotte | Mason Menassa, Ethan Lukow, Braden Valdez, Gaston Salvador | Jacob Canup, Porter Crawford, Kelby Piper, Noah Gergley | 1:09:40.9713 | 50:10:26.3464 | - | - |
| 12. | 13. | Gateway (Fort Myers) | Dunbar | Connor Rees, Naseem Abujudeh, Malachi Vann, Ricardo Charite | Connor Rees, Naseem Abujudeh, Malachi Vann, Ricardo Charite | 4:09:49.837 | 8:22.144 | - | - |
| 13. | 9. | Island Coast | Gateway (Fort Myers) | Caeleb Spriggs, Amare Archie, Jesus Us-Tiu, Christopher Singleton | Connor Rees, Naseem Abujudeh, Malachi Vann, Ricardo Charite | 9:52.317 | 8:44.25 | - | - |
| 14. | 16. | Port Charlotte | Cape Coral | Isaac Magallanes, Brendan Flavin, Christopher Hausler, Andrew Light | Mason Menassa, Ethan Lukow, Braden Valdez, Gaston Salvador | 52:10:11.007 | 52:08:32.7059 | - | - |
| 15. | 11. | East Lee County | Cape Coral | David Crome, Ever Galeas, Fernando Mendoza, Florentino Izaguirre | Ryan Livingston, Daniel Ocampo, Ivan Marin-Padilla, Olmar Bartolon | 10:18.888 | 52:10:11.007 | - | 95 |
| 16. | 16. | Bonita Springs | Island Coast | Omar Buenrostro, Brian Altuve- Viloria, Ethan Knize, Freddy Garrido | Omar Buenrostro, Brian Altuve- Viloria, Ethan Knize, Freddy Garrido | 50:10:26.3464 | 8:52.9948 | - | - |
