| 1. | 11. | 15. | Canterbury (FM) | Shorecrest Prep | Indian Rocks Christian | Giana D'Altrui, Michelle Barnes, Ellie Miller, Jessica Edwards | Kelis Sims, Tytiana Wilson, Denia Averette, Tekoyia Cheedy | Calista Mason, Hannah Hudson, Hoshea Joseph, Daniela Cardenas | 4:02.136 | 33:04:04.2968 | 4:03.15 | 109 | 2441 | 149 |
| 2. | 3. | 5. | Admiral Farragut | Maclay | Master's Academy (Oviedo) | Kelis Sims, Tytiana Wilson, Denia Averette, Tekoyia Cheedy | Aaliyah Smith, Kendra Pruitt, Ava Melancon, Avery Erikson | Kasey Davey, Eliana Curry, Lindy White, Skyy Dixon | 4:03.15 | 17:04:18.91 | 4:20.81 | 3780 | - | - |
| 3. | 2. | 11. | Wildwood | Palmer Trinity | First Baptist (Naples) | Kendall Brown, Casey Williams, Ja'Kyra Corbin, Ranyla Jackson | Grace Coggins, Riley McKinney, Marissa Earley, Payton West | Emilee McPherson, Macy Fekkers, Mori McCain, Elianna Christensen | 33:04:04.2968 | 4:23.8806 | 4:02.136 | 361 | - | - |
| 4. | 2. | 10. | Oak Hall | Admiral Farragut | First Academy (Orlando) | Gabriella Aulisio, Eliana Eisner, Lauren Jones, Sydney Miller | Giana D'Altrui, Michelle Barnes, Ellie Miller, Jessica Edwards | Kelis Sims, Tytiana Wilson, Denia Averette, Tekoyia Cheedy | 48:04:08.647 | 4:20.452 | 4:16.04 | 50 | - | 361 |
| 5. | 12. | 5. | Indian Rocks Christian | Canterbury (FM) | Palmer Trinity | Ava Labrant, Kaci Anderson, Ayden Martin, Rebecca Dumm | Lilly Nava, Ella Clements, Shyanne Scott, Kiara Davis | Alexa Wilson, Maria Newman, Ryleigh Wilson, Ava Wyant | 4:09.42 | 21:04:22.39 | 17:04:18.91 | 2441 | - | - |
| 6. | 4. | 5. | Maclay | Admiral Farragut | St. Stephens Episcopal | Daniela Paredes, Isabella Barton, Rachel Bethke, Amelia Haggins | Giana D'Altrui, Michelle Barnes, Ellie Miller, Jessica Edwards | Giana D'Altrui, Michelle Barnes, Ellie Miller, Jessica Edwards | 20:04:15.7717 | 26:04:20.03 | 33:04:04.2968 | 903 | - | 3780 |
| 7. | 2. | 5. | Palmer Trinity | Master's Academy (Oviedo) | Maclay | Maria Camposano, Angelina Deleon, Noelani Sadler, Gaby Dorta | Alanis Job, Kaja Dionne, Alex Estremera, Ashli McKenny | Ava Labrant, Kaci Anderson, Ayden Martin, Rebecca Dumm | 4:16.04 | 4:16.04 | 4:20.81 | 772 | 361 | - |
| 8. | 4. | 9. | Westminster Academy | Indian Rocks Christian | Admiral Farragut | Lilly Nava, Ella Clements, Shyanne Scott, Kiara Davis | Catherine Lamprecth, Janie Chatham, Nikole Cosentino, Brooke Cosentino | Gabriella Aulisio, Eliana Eisner, Lauren Jones, Sydney Miller | 17:04:18.91 | 4:31.01 | 4:22.1637 | 149 | - | - |
| 9. | 8. | 15. | St. Stephens Episcopal | First Academy (Orlando) | First Baptist (Naples) | Catherine Lamprecth, Janie Chatham, Nikole Cosentino, Brooke Cosentino | Ava Labrant, Kaci Anderson, Ayden Martin, Rebecca Dumm | Catherine Lamprecth, Janie Chatham, Nikole Cosentino, Brooke Cosentino | 26:04:20.03 | 26:04:20.03 | 4:20.452 | - | - | - |
| 10. | 12. | 1. | Master's Academy (Oviedo) | Admiral Farragut | Oak Hall | Alexa Wilson, Maria Newman, Ryleigh Wilson, Ava Wyant | Kelis Sims, Tytiana Wilson, Denia Averette, Tekoyia Cheedy | Daniela Paredes, Isabella Barton, Rachel Bethke, Amelia Haggins | 4:20.452 | 58:04:24.60 | 4:16.04 | - | - | - |
| 11. | 12. | 15. | First Baptist (Naples) | Wildwood | Oak Hall | Aaliyah Smith, Kendra Pruitt, Ava Melancon, Avery Erikson | Ava Labrant, Kaci Anderson, Ayden Martin, Rebecca Dumm | Maria Camposano, Angelina Deleon, Noelani Sadler, Gaby Dorta | 4:20.81 | 58:04:24.60 | 4:20.81 | - | - | 109 |
| 12. | 3. | 5. | Shorecrest Prep | Rocky Bayou Christian | Providence | Alanis Job, Kaja Dionne, Alex Estremera, Ashli McKenny | Aaliyah Smith, Kendra Pruitt, Ava Melancon, Avery Erikson | Aaliyah Smith, Kendra Pruitt, Ava Melancon, Avery Erikson | 4:22.1637 | 4:09.42 | 20:04:15.7717 | - | 361 | - |
| 13. | 2. | 1. | First Academy (Orlando) | Wildwood | Westminster Academy | Calista Mason, Hannah Hudson, Hoshea Joseph, Daniela Cardenas | Emilee McPherson, Macy Fekkers, Mori McCain, Elianna Christensen | Gabriella Aulisio, Eliana Eisner, Lauren Jones, Sydney Miller | 21:04:22.39 | 26:04:20.03 | 48:04:08.647 | - | 50 | - |
| 14. | 13. | 1. | Southwest Florida Christian | Canterbury (FM) | Providence | Grace Coggins, Riley McKinney, Marissa Earley, Payton West | Grace Coggins, Riley McKinney, Marissa Earley, Payton West | Catherine Lamprecth, Janie Chatham, Nikole Cosentino, Brooke Cosentino | 4:23.8806 | 4:03.15 | 4:23.8806 | - | - | - |
| 15. | 14. | 11. | Rocky Bayou Christian | St. Stephens Episcopal | Rocky Bayou Christian | Emilee McPherson, Macy Fekkers, Mori McCain, Elianna Christensen | Grace Coggins, Riley McKinney, Marissa Earley, Payton West | Ava Labrant, Kaci Anderson, Ayden Martin, Rebecca Dumm | 58:04:24.60 | 4:31.01 | 4:03.15 | - | 772 | 3780 |
| 16. | 10. | 3. | Providence | Westminster Academy | Westminster Academy | Kasey Davey, Eliana Curry, Lindy White, Skyy Dixon | Grace Coggins, Riley McKinney, Marissa Earley, Payton West | Emilee McPherson, Macy Fekkers, Mori McCain, Elianna Christensen | 4:31.01 | 4:31.01 | 21:04:22.39 | - | - | - |
