1 | Davila , Keshawn | SR | Hillsborough | 49.10 | FHSAA 3A Region 3 | May 6, 2022 |
| 2 | McClendon, Joshua | JR | Sumner | 50.07 | Sumner Quad Meet #2 | Mar 9, 2022 |
| 3 | Metcalf, Brandon | SO | Sumner | 50.83 | 2022 Hillsborough County Championships | Apr 13, 2022 |
| 4 | Bowen, Ammiel | JR | King | 51.39 | FHSAA 3A Region 3 | May 6, 2022 |
| 5 | Clyde, Nathaniel | SR | Spoto | 51.50 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 6 | Exceus, Jeremiah | SR | Sumner | 51.63 | 2022 Hillsborough County Championships | Apr 13, 2022 |
| 7 | Johnson, Lakeem | SO | Robinson | 51.68 | FHSAA 3A Region 3 | May 6, 2022 |
| 8
| Mooney, Patrick | SR | Hillsborough | 52.76 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 9
| Smith, Gregory | SO | Sumner | 52.85 | Sumner Quad Meet #2 | Mar 9, 2022 |
| 10 | Richardson, Justin | JR | Robinson | 53.28 | Raven Invitational 2022 | Mar 19, 2022 |
| 11 | Grant, Tyler | FR | Sumner | 53.39 | Sumner Senior Night Meet | Mar 30, 2022 |
| 12 | Hodges, Jalen | JR | Sumner | 53.43 | Sumner Quad Meet #2 | Mar 9, 2022 |
| 13 | Preston-McCluster, Wendell | JR | Blake | 53.56 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 14 | Mayak, Jacob | JR | Sumner | 53.58 | Sumner Quad Meet #1 | Feb 22, 2022 |
| 15 | Brewer, Gabriel | SR | Hillsborough | 53.66 | National Division Meet | Apr 6, 2022 |
| 16 | Sureller, Clerance | FR | Sumner | 54.07 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 17 | Brown, Sean | JR | Hillsborough | 54.20 | George Steinbrenner Invitational | Apr 9, 2022 |
| 18 | Johnson , Trayvun | JR | King | 54.24 | FHSAA 3A District 9 | Apr 26, 2022 |
| 19 | Mack Jr, Rod | FR | Jefferson | 54.31 | FHSAA 3A District 9 | Apr 26, 2022 |
| 20 | Colbert, Michael | JR | Blake | 54.53 | Leto 2/25/2022 4 Way Meet | Feb 25, 2022 |
| 21 | King, O'Darius | JR | Sumner | 54.54 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 22 | Joyce, Deyon | JR | King | 54.57 | Raven Invitational 2022 | Mar 19, 2022 |
| 23 | Epps, Ja'Mez | JR | Leto | 55.17 | WC American Divisionals | Apr 6, 2022 |
| 24 | Jackson, Antwan | SR | East Bay | 55.34 | FHSAA 3A District 9 | Apr 26, 2022 |
| 25 | Jones, Marcus | SR | East Bay | 55.84 | Sumner Senior Night Meet | Mar 30, 2022 |
| 26 | Smith, Ian | SR | Hillsborough | 56.17 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 27 | Osborne, Jay'von | FR | Spoto | 56.20 | Spoto Quad Meet 1 | Feb 23, 2022 |
| 28 | Houston, Deshawn | SR | Brandon | 56.23 | FHSAA 3A District 9 | Apr 26, 2022 |
| 29 | Hermosillo, Ruben | JR | Spoto | 56.33 | Western Conference - Federal Division | Apr 6, 2022 |
| 30 | Reaves, Dominic | SR | Hillsborough | 56.38 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 31 | Collins, Aaron | FR | Robinson | 56.40 | 2022 4 Way | Mar 31, 2022 |
| 32 | Anderson, Justin | SR | Hillsborough | 56.52 | FHSAA 3A District 9 | Apr 26, 2022 |
| 33 | Burney, Zhonte | JR | Sumner | 56.67 | Sumner Senior Night Meet | Mar 30, 2022 |
| 34 | Brown, Kevin | SO | Middleton | 56.72 | Spoto Quad Meet 3 | Mar 30, 2022 |
| 35 | Bogan, Xavier | SO | Hillsborough | 56.78 | George Steinbrenner Invitational | Apr 9, 2022 |
| 36 | Brown, Kevin | SO | Middleton | 56.84 | National Division Meet | Apr 6, 2022 |
| 37 | Rivera, Wendell | JR | Jefferson | 56.91 | FHSAA 3A District 9 | Apr 26, 2022 |
| 38 | Greenwood, Bryan | FR | Sumner | 56.96 | Sumner Quad Meet #1 | Feb 22, 2022 |
| 39 | Schumacher, Nathan | FR | Robinson | 56.97 | WC American Divisionals | Apr 6, 2022 |
| 40 | Cotton, Zorey | FR | Spoto | 57.00 | FHSAA 3A District 9 | Apr 26, 2022 |
| 41 | Demard , Rodrigue | JR | Robinson | 57.09 | 2022 4 Way | Mar 31, 2022 |
| 42 | Fernandez, Lazaro | SO | Leto | 57.10 | FHSAA 3A District 9 | Apr 26, 2022 |
| 43 | McCray, Kelvin | JR | Robinson | 57.11 | 4 Way Meet | Mar 9, 2022 |
| 44 | Keats, Blair | FR | Robinson | 57.28 | George Steinbrenner Invitational | Apr 9, 2022 |
| 45 | Wilburn, William | SO | Robinson | 57.31 | 4 Way Meet | Mar 9, 2022 |
| 46 | Oliver, Dajohn | SO | Middleton | 57.39 | Bloomingdale Track Meet | Feb 22, 2022 |
| 47 | Acevedo, Adolfo | SR | Brandon | 57.46 | Sumner Quad Meet #2 | Mar 9, 2022 |
| 48 | Kanu, Henry | JR | Blake | 57.54 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 49 | Fernandez, Jeremiah | JR | Robinson | 57.67 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 50 | Hobbs, Quamar | JR | Leto | 57.91 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 51 | Velasquez , Elijah | FR | King | 58.01 | Sumner Quad Meet #1 | Feb 22, 2022 |
| 52 | Jones, Zyon | FR | Blake | 58.30 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 53 | Camilo, Gabriel | JR | Blake | 58.39 | WC American Divisionals | Apr 6, 2022 |
| 54 | Lineberger, Nick | SR | East Bay | 58.40 | Sumner Senior Night Meet | Mar 30, 2022 |
| 55 | Webster, Jaylen | SR | East Bay | 58.47 | Bloomingdale Track Meet | Feb 22, 2022 |
| 56 | Carr, Jaden | FR | Hillsborough | 58.49 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 57 | Fagan, Jaquarius | JR | Brandon | 58.50 | Western Conference - Federal Division | Apr 6, 2022 |
| 58 | Hayes, Jaydon | SR | Sumner | 58.69 | Spoto Quad Meet 2 | Mar 2, 2022 |
| 59 | Kennison, Layton | FR | Sumner | 58.73 | Sumner Senior Night Meet | Mar 30, 2022 |
| 60 | Robinson, Antrel | JR | Middleton | 59.02 | FHSAA 3A District 9 | Apr 26, 2022 |
| 61 | Tyson, Jarian | JR | Middleton | 59.19 | Spoto Quad Meet 3 | Mar 30, 2022 |
| 62 | Neely, Xavion | FR | Spoto | 59.65 | Spoto Quad Meet 3 | Mar 30, 2022 |
| 63 | Marcelin, Elijah | FR | Sumner | 59.85 | Spoto Quad Meet 2 | Mar 2, 2022 |
| 64 | Turner, Isaiah | SO | Spoto | 59.86 | Sumner Quad Meet #2 | Mar 9, 2022 |
| 65 | Kombate, Godwin | SO | King | 59.95 | National Division Meet | Apr 6, 2022 |
| 66 | Robinson, Antrel | JR | Middleton | 1:00.07 | Spoto Quad Meet 3 | Mar 30, 2022 |
| 67 | Brewer, Nathan | SO | Hillsborough | 1:00.17 | FHSAA 3A District 9 | Apr 26, 2022 |
| 67 | Sank, Jeremiah | FR | Hillsborough | 1:00.17 | 4 Way Meet | Mar 9, 2022 |
| 69 | Brantley, Kazzie | FR | Blake | 1:00.20 | Leto 2/25/2022 4 Way Meet | Feb 25, 2022 |
| 70 | Mendez, Julez (Santi) | SO | Brandon | 1:00.42 | Bloomingdale Meet #2 | Mar 2, 2022 |
| 71 | Anderson, Shayvon | FR | Leto | 1:00.54 | FHSAA 3A District 9 | Apr 26, 2022 |
| 72 | Miller, Thomas | SO | Hillsborough | 1:00.95 | Jesuit Quad Meet 2 | Mar 2, 2022 |
| 73 | Martin, Elvis | SR | Hillsborough | 1:00.98 | National Division Meet | Apr 6, 2022 |
| 74 | Albert, Mikenson | JR | Middleton | 1:01.05 | Bloomingdale Track Meet | Feb 22, 2022 |
| 75 | Tyson, Jarian | JR | Middleton | 1:01.29 | Bloomingdale Track Meet | Feb 22, 2022 |
| 76 | Williams, Javian | SO | Jefferson | 1:01.32 | FHSAA 3A District 9 | Apr 26, 2022 |
| 77 | Nelson , Darnell | FR | Blake | 1:01.36 | 2022 4 Way | Mar 31, 2022 |
| 78 | Wilson, Aaron | SR | Blake | 1:01.42 | Leto 2/25/2022 4 Way Meet | Feb 25, 2022 |
| 79 | O'Brien, Markel | JR | East Bay | 1:01.45 | Western Conference - Federal Division | Apr 6, 2022 |
| 80 | Madison, Sykhi | SO | Jefferson | 1:01.71 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 81 | Vandyck, Cole | SR | Blake | 1:01.80 | FHSAA 3A District 9 | Apr 26, 2022 |
| 82 | Morgan, Joshua | SO | Leto | 1:01.99 | WC American Divisionals | Apr 6, 2022 |
| 83 | Garcia, Caleb | JR | Brandon | 1:02.17 | Sumner Quad Meet #2 | Mar 9, 2022 |
| 84 | Philogene, Denario | SO | Leto | 1:02.48 | 4 Way Meet | Mar 9, 2022 |
| 85 | Carrsquillo, Joel | SR | Middleton | 1:02.50 | Bloomingdale Track Meet | Feb 22, 2022 |
| 86 | Mondragon, Miguel | JR | Middleton | 1:02.66 | Bloomingdale Track Meet | Feb 22, 2022 |
| 87 | Ensor, Devin | JR | East Bay | 1:03.15 | Bloomingdale Track Meet | Feb 22, 2022 |
| 88 | Reguenard, Royce | JR | Middleton | 1:03.41 | FHSAA 3A District 9 | Apr 26, 2022 |
| 89 | Huttie, Chris | JR | East Bay | 1:03.57 | Bloomingdale Track Meet | Feb 22, 2022 |
| 90 | Stabler, Jordan | SO | Middleton | 1:03.79 | Bloomingdale Track Meet | Feb 22, 2022 |
| 91 | Jefferson, Quentin | SO | Hillsborough | 1:03.91 | 4 Way Meet | Mar 9, 2022 |
| 92 | Ojo, David | SO | Middleton | 1:04.09 | Bloomingdale Track Meet | Feb 22, 2022 |
| 93 | Barnes, Dominick | SO | Leto | 1:04.20 | Leto 2/25/2022 4 Way Meet | Feb 25, 2022 |
| 94 | Perkins, Kyle | FR | Robinson | 1:05.06 | FHSAA 3A District 9 | Apr 26, 2022 |
| 95 | Smith, Mareeb | JR | Middleton | 1:05.08 | Bloomingdale Track Meet | Feb 22, 2022 |
| 96 | Braxton, Tyre | FR | Spoto | 1:05.27 | Spoto Quad Meet 2 | Mar 2, 2022 |
| 97 | Grover, Chandler | JR | Middleton | 1:05.64 | Bloomingdale Track Meet | Feb 22, 2022 |
| 98 | Alexis, Zion | SO | Brandon | 1:06.02 | Leto - Nash Higgins Relays 2022 | Mar 5, 2022 |
| 99 | Uribe, Fernando | SO | Spoto | 1:06.39 | Spoto Quad Meet 2 | Mar 2, 2022 |
| 100 | Morrison , Alonza | SO | East Bay | 1:07.95 | Sumner Senior Night Meet | Mar 30, 2022 |
FHSAA 3A