| 1. | 10. | Oak Hall | Bell | Eliana Eisner, Lauren Jones, Sydney Miller, Alivia Dragstedt | Moriah Vann, Hannah Vann, Keely Bolling, Victoria Everts | 22:09:47.3162 | 11:11:18.41 | 6510 | - |
| 2. | 6. | Circle Christian | St. Francis | Sarah Rudolph, Mia Carlson, Abigail Fagot, Kaela Williams | Rachel Horsford, Ella Mast, Audrey McConnell, Belle Orser | 9:54.39 | 10:38.60 | 6886 | 19 |
| 3. | 10. | Master's Academy (Oviedo) | Master's Academy (Oviedo) | Haleigh Silver, Ryleigh Wilson, Ava Wyant, Jessica Merly | Addison Lunn, Isabella Kreinbihl, Rayanah Mkuu, Riley Whitaker | 10:33.0182 | 11:10.9426 | 168 | 21 |
| 4. | 5. | St. Francis | Master's Academy (Oviedo) | Millie Adin, Mica Krushinskie, Tommie Bost, Catalina Romero | Haleigh Silver, Ryleigh Wilson, Ava Wyant, Jessica Merly | 10:38.60 | 22:09:47.3162 | 85585 | 21 |
| 5. | 11. | Mount Dora Christian | Master's Academy (Oviedo) | Rachel Horsford, Ella Mast, Audrey McConnell, Belle Orser | Liani Gaud, Addison Davis, Kaley Earl, Eva Elder | 8:11:05.01 | 11:10.9426 | 403 | 6886 |
| 6. | 4. | St. Joseph | Circle Christian | Paige Brammer, Meaganne Doctora, Kira Casane, Ana Whitehouse | Addison Lunn, Isabella Kreinbihl, Rayanah Mkuu, Riley Whitaker | 11:10.9426 | 10:38.60 | 32 | 403 |
| 7. | 12. | City of Life Christian | First Academy (Orlando) | Angelina Garrett, Kaylee Harkema, Angela Lopez, Valeria Gonzalez | Calista Mason, Jewel Robinson, Sarah Jackson, Madaline McGinn | 54:11:11.2745 | 11:14.3117 | 21 | 403 |
| 8. | 6. | First Academy (Orlando) | Foundation Academy | Calista Mason, Jewel Robinson, Sarah Jackson, Madaline McGinn | Millie Adin, Mica Krushinskie, Tommie Bost, Catalina Romero | 11:14.3117 | 8:11:05.01 | 19 | 32 |
| 9. | 7. | Branford | First Academy (Orlando) | Jessie Sikes, Callie Harris, Kayleigh Wardle, Kayley Johnson | Paige Brammer, Meaganne Doctora, Kira Casane, Ana Whitehouse | 11:11:18.41 | 22:09:47.3162 | - | 19 |
| 10. | 6. | Bell | Mount Dora Christian | Addison Lunn, Isabella Kreinbihl, Rayanah Mkuu, Riley Whitaker | Jessie Sikes, Callie Harris, Kayleigh Wardle, Kayley Johnson | 11:29.681 | 10:38.60 | - | 6510 |
| 11. | 2. | Foundation Academy | Bell | Liani Gaud, Addison Davis, Kaley Earl, Eva Elder | Eliana Eisner, Lauren Jones, Sydney Miller, Alivia Dragstedt | 11:43.45 | 54:11:11.2745 | - | 6510 |
| 12. | 11. | Seven Rivers Christian | St. Francis | Moriah Vann, Hannah Vann, Keely Bolling, Victoria Everts | Addison Lunn, Isabella Kreinbihl, Rayanah Mkuu, Riley Whitaker | 11:48.34 | 11:48.34 | - | 6510 |
