| 1. | 10. | Holy Names | Tarpon Springs | Britton Byrd, Chloe Carson, Elizabeth Williamson, Lillian Hund | Kate Anderson, Carsyn Martz, Emma Flowers, Anna McDermott | 26:09:43.47 | 25:11:25.80 | 1089 | 97858 |
| 2. | 13. | Berkeley Prep | Bishop Verot | Kate Anderson, Carsyn Martz, Emma Flowers, Anna McDermott | Madelynn Sherrill, Evelyn Quiroz, Teziah Pierre-Lewis, Jayleen Reyes | 43:09:47.9111 | 41:10:11.5267 | 97858 | - |
| 3. | 3. | Bishop Verot | Bell Creek | Mackenzie De Lisle, Hannah Manibo, Julia Reitz, Kylie Thomas | Emily Maul, Megan Miller, Alayna Mann, Abigail Ryals | 35:09:55.0345 | 11:15.31 | 816 | 816 |
| 4. | 4. | Calvary Christian (Clr) | Lake Region | Lauren Mahan, Hannah Kasper, Merritt Smith, Hailey Howard | Jenny Swenson, Rebecca Jackson, Emmie Backman, Isabella Maccini | 10:10.807 | 12:17.61 | 25 | 97858 |
| 5. | 4. | Bell Creek | Bell Creek | Olivia Orobello, Riana Pontemayor, Bella Butler, Alexia Tine | Britton Byrd, Chloe Carson, Elizabeth Williamson, Lillian Hund | 41:10:11.5267 | 43:09:47.9111 | 47478 | - |
| 6. | 3. | Parrish | Lake Region | Jenny Swenson, Rebecca Jackson, Emmie Backman, Isabella Maccini | Jenny Swenson, Rebecca Jackson, Emmie Backman, Isabella Maccini | 52:10:58.2713 | 38:11:13.070 | 2636 | 1089 |
| 7. | 5. | McKeel | Lemon Bay | Emily Maul, Megan Miller, Alayna Mann, Abigail Ryals | Lauren Mahan, Hannah Kasper, Merritt Smith, Hailey Howard | 38:11:13.070 | 35:09:55.0345 | 2 | 1089 |
| 8. | 2. | Tarpon Springs | Lake Region | Bayley Wisniewski, Halie Musco, Mia Jarrell, Kyleigh Faison | Britton Byrd, Chloe Carson, Elizabeth Williamson, Lillian Hund | 11:15.31 | 11:15.31 | 164 | 1089 |
| 9. | 6. | Hardee | Bishop Verot | Jasmin Aviles, Yadira Sanchez, Adrianna Mier, Ava Roberts | Mackenzie De Lisle, Hannah Manibo, Julia Reitz, Kylie Thomas | 25:11:25.80 | 38:11:13.070 | - | 97858 |
| 10. | 6. | Lemon Bay | Port Charlotte | Charlotte Carley, Maliaya Long, Haylee Marsicovetere, Addison Morey | Jasmin Aviles, Yadira Sanchez, Adrianna Mier, Ava Roberts | 51:11:47.09 | 51:11:47.09 | - | - |
| 11. | 1. | Port Charlotte | Bishop Verot | Lorrena Wittmann, Susana Rodriguez, Ayahna Rosado, Abigail Hass | Emily Maul, Megan Miller, Alayna Mann, Abigail Ryals | 12:17.61 | 25:11:25.80 | - | - |
| 12. | 3. | Lake Placid | Lake Region | Juliana Tamayo-Gurrero, Sayra Cortes, Dayanara Jiminez Media, Solymar Alvarez-Cruz | Madelynn Sherrill, Evelyn Quiroz, Teziah Pierre-Lewis, Jayleen Reyes | 36:12:44.00 | 25:11:25.80 | - | 97858 |
| 13. | 5. | Lake Region | Calvary Christian (Clr) | Madelynn Sherrill, Evelyn Quiroz, Teziah Pierre-Lewis, Jayleen Reyes | Kate Anderson, Carsyn Martz, Emma Flowers, Anna McDermott | 13:43.2427 | 36:12:44.00 | - | - |
