| 1. | 4. | Alonso | East Lake | Kevin Velazquez, Ryan Winslow, Raekwon Hopkins, Ian Ludlam | Angelo Cavalleri, Nicholas Jacobs, William Carter, Micah Snare | 8:09.41 
| 8:09.41
| 9109 | 5 |
| 2. | 5. | Palm Harbor University | George Steinbrenner | Mark Amin, Jack Penn, Matthew Gunn, Max Moore | Charles Jennessee, Zachary Glover, Abhinav Devpura, Cooper Chesser | 8:10.717
| 9:01.193 | 98 | 98 |
| 3. | 3. | Largo | George Steinbrenner | David Denkler, David Newton, Joshua Pu, Abdu Hardin | Kevin Velazquez, Ryan Winslow, Raekwon Hopkins, Ian Ludlam | 8:13.108
| 8:10.717
| 64 | 98 |
| 4. | 6. | Wiregrass Ranch | Wharton | Mason Spearin, Jaime Candelaria, Tyler Dana, Joseph Medina | Angelo Cavalleri, Nicholas Jacobs, William Carter, Micah Snare | 28:08:13.438
| 9:01.193 | 5 | 64 |
| 5. | 3. | George Steinbrenner | East Lake | Ronald Aeschleman, Jaden Simpson, Joaquin Abanses, Eion McDarby | Ronald Aeschleman, Jaden Simpson, Joaquin Abanses, Eion McDarby | 59:08:22.81 | 50:09:12.90 | 427 | 427 |
| 6. | 5. | Sickles | East Lake | Charles Jennessee, Zachary Glover, Abhinav Devpura, Cooper Chesser | Charles Jennessee, Zachary Glover, Abhinav Devpura, Cooper Chesser | 38:08:49.056 | 8:13.108
| 68366 | 98 |
| 7. | 5. | Wharton | George Steinbrenner | Brett Brown, Dane Deevers, Emanuel Camacho, Cade Toohey | Charles Jennessee, Zachary Glover, Abhinav Devpura, Cooper Chesser | 9:01.193 | 8:13.108
| 1207 | 68366 |
| 8. | 8. | East Lake | George Steinbrenner | Angelo Cavalleri, Nicholas Jacobs, William Carter, Micah Snare | Brett Brown, Dane Deevers, Emanuel Camacho, Cade Toohey | 50:09:12.90 | 50:09:12.90 | 71 | 98 |
Region Qualifier