| 1. | 2. | Evans | Evans | Mariama Kamara, Abrianna Scott, Queen Boyer, Ar'briel Scott | Mariama Kamara, Abrianna Scott, Queen Boyer, Ar'briel Scott | 48:03:56.333 
| DNS | 1079 | - |
| 2. | 1. | Ocoee | Winter Park | Bertina Jean, Jarvia Carr, Lourdes Safford, Alyia Green | Olivia Denney, Jazlyn Dailey, Patley Bernard, Eliana Oquendo | 20:03:58.3163
| DNS | 9895 | 25 |
| 3. | 5. | West Orange | Apopka | Ayla Fisher, Jasymnne Gibson, Jasmine Moore, Nasjai White | Hailey Fowler, Olivia Eugene, Hannah Jones, DeMaris Soto | 4:05.62
| 44:04:17.174 | 56 | 9895 |
| 4. | | Apopka | Winter Park | Hailey Fowler, Olivia Eugene, Hannah Jones, DeMaris Soto | Emani Glover, Madelyn Vargas, Andrea Waite, Neely Lugo-Ramos | 49:04:10.7762
| DNS | 25 | 9895 |
| 5. | 2. | Winter Park | Apopka | Olivia Denney, Jazlyn Dailey, Patley Bernard, Eliana Oquendo | Bertina Jean, Jarvia Carr, Lourdes Safford, Alyia Green | 44:04:17.174 | 49:04:10.7762
| 54 | 9895 |
| | 5. | East Ridge | East Ridge | Emani Glover, Madelyn Vargas, Andrea Waite, Neely Lugo-Ramos | Olivia Denney, Jazlyn Dailey, Patley Bernard, Eliana Oquendo | DNS | 48:03:56.333
| - | 56 |
Region Qualifier