| 1. | 6. | Wildwood | Seven Rivers Christian | Ranyla Jackson, Hannah Serfass, Emma Strickland, Casey Williams | Kaela Williams, Abigail Fagot, Mia Carlson, Sarah Rudolph | 4:13.8646 
| 4:55.66 | 341054 | 341054 |
| 2. | 4. | Circle Christian | First Academy (Leesburg) | Kaela Williams, Abigail Fagot, Mia Carlson, Sarah Rudolph | Kaela Williams, Abigail Fagot, Mia Carlson, Sarah Rudolph | 1:04:22.773
| 14:04:29.07
| 2280 | 341054 |
| 3. | 3. | First Academy (Leesburg) | Mount Dora Christian | Molly Beliveau, Emma Fought, Sophia Arzu, Ella Iseminger | Robyn Stephens, Livia Noyes, Amelia Baker, Abigail Longstaff | 17:04:24.400
| 4:55.66 | 606 | 8046 |
| 4. | 6. | Seven Rivers Christian | Wildwood | Jurisah Bailey, McKala Peters, Emily Eden, Nora Leonard | Ranyla Jackson, Hannah Serfass, Emma Strickland, Casey Williams | 14:04:29.07
| 4:13.8646
| 9556 | 341054 |
| 5. | 1. | Orangewood Christian | Orangewood Christian | Robyn Stephens, Livia Noyes, Amelia Baker, Abigail Longstaff | Rachel Horsford, Ella Mast, Leah Sowers, Reese Horbal | 4:30.807 | 4:55.66 | 8046 | 633 |
| 6. | 6. | Mount Dora Christian | Mount Dora Christian | Rachel Horsford, Ella Mast, Leah Sowers, Reese Horbal | Avery Bangsund, Haven Brookins, Claire Reburn, Kaley Earl | 3:04:47.0044 | 4:30.807 | 633 | 2280 |
| 7. | 7. | Foundation Academy | Circle Christian | Avery Bangsund, Haven Brookins, Claire Reburn, Kaley Earl | Jurisah Bailey, McKala Peters, Emily Eden, Nora Leonard | 4:55.66 | 4:13.8646
| 213 | 633 |
Region Qualifier