| 1. | | Ocoee | St. Thomas Aquinas | Miracle Johnson, Jarvia Carr, Lourdes Safford, Alyia Green | Nyla McFayden, Olivia Eugene, Hailey Fowler, DeMaris Soto | 3:52.4256 | 3:56.0222 | 61027 | - |
| 2. | 2. | St. Thomas Aquinas | Davenport | Morgan Crockett, Endeyah Lane, Shanel Morgan, Cha'iel Johnson | Aaliyah Lamar, Ryla Bryant, Isabella Alcantara, Ayanna Diejuste | 3:56.0222 | 4:05.9986 | 98 | 756 |
| 3. | | Evans | Wekiva | Queen Boyer , Abrianna Scott, Alisha Boyer, Kemaj Moore | Shannon Aguilar, Bianca Chavannes, Sarah Laguerre, Deanna Harley | 3:59.926 | DNS | 756 | 01379 |
| 4. | | West Orange | East Ridge | Ayla Fisher, Nasjai White, Sophia Joseph, Jasymnne Gibson | Queen Boyer , Abrianna Scott, Alisha Boyer, Kemaj Moore | 4:05.9986 | 3:59.926 | 705 | - |
| 5. | 1. | Apopka | Lake Gibson | Nyla McFayden, Olivia Eugene, Hailey Fowler, DeMaris Soto | AnnaRose Wheatle, Andrea Waite, Emani Glover, Yamina Numan | 4:15.53 | 3:52.4256 | 4 | - |
| 6. | 4. | Jones | St. Thomas Aquinas | kierstin hall, Ja'Mese Heard, Lajada Williams , nicole shorter | Anika Vidrine, Carly Kahn, Meryl Canine, Vivian Vogel | 4:04:19.5281 | 3:56.0222 | 01379 | - |
| 7. | | East Ridge | Apopka | AnnaRose Wheatle, Andrea Waite, Emani Glover, Yamina Numan | Anika Vidrine, Carly Kahn, Meryl Canine, Vivian Vogel | 4:19.6567 | 3:59.926 | 02 | - |
| 8. | 12. | Olympia | Lake Gibson | Maya Abraham, Abby Misch, Nauti'ka Robinson, Anaya Vil | Ayla Fisher, Nasjai White, Sophia Joseph, Jasymnne Gibson | 17:04:20.42 | DNS | 13 | - |
| 9. | 4. | Edgewater | East Ridge | Decoursha Riley, Bailey Stokes, Casey Estrada, Johanna Pineiro | Anika Vidrine, Carly Kahn, Meryl Canine, Vivian Vogel | 4:39.5871 | DNS | - | - |
| 10. | | Freedom (Orlando) | Ocoee | Shannon Aguilar, Bianca Chavannes, Sarah Laguerre, Deanna Harley | Darieliz Llanos, Lakisha Manning, Jayla Chambers, Megan Dolan | 16:04:44.69 | DNS | - | 61027 |
| 11. | | Lake Gibson | St. Thomas Aquinas | Aaliyah Lamar, Ryla Bryant, Isabella Alcantara, Ayanna Diejuste | Darieliz Llanos, Lakisha Manning, Jayla Chambers, Megan Dolan | 4:44.76 | DNS | - | - |
| 12. | 6. | Foundation Academy | Freedom (Orlando) | Avery Bangsund, Kaley Earl, Claire Reburn, Kate Rosenthal | Aaliyah Lamar, Ryla Bryant, Isabella Alcantara, Ayanna Diejuste | 11:04:53.094 | 11:04:53.094 | - | 4 |
| 13. | | Patel | Freedom (Orlando) | Ra'Kiema Wingfield, Anallei Perez Campa, Sofia Caballero, Mona Barbary | Queen Boyer , Abrianna Scott, Alisha Boyer, Kemaj Moore | 4:58.6804 | 4:19.6567 | - | 756 |
| | | Davenport | Davenport | Tyanna Jones, Arleana Chong, Jamari Gallard, Kerionne Wiley | Jocelyn Townsend, Ashley Blanco, Jolisa Howard, Markia McCarthy | DNS | DNS | - | 13 |
| | 9. | Windermere | Davenport | Kaelynn Fitos, Emma Harris , Lauren Perkins, Ryan Stevenson | Maya Abraham, Abby Misch, Nauti'ka Robinson, Anaya Vil | DNS | 3:56.0222 | - | 01379 |
| | 12. | Lake Highland Prep | Patel | Anika Vidrine, Carly Kahn, Meryl Canine, Vivian Vogel | Anika Vidrine, Carly Kahn, Meryl Canine, Vivian Vogel | DNS | 4:44.76 | - | 13 |
| | 3. | Wekiva | Apopka | Jocelyn Townsend, Ashley Blanco, Jolisa Howard, Markia McCarthy | Nyla McFayden, Olivia Eugene, Hailey Fowler, DeMaris Soto | DNS | DNS | - | 01379 |
| | 12. | Boone | St. Thomas Aquinas | Darieliz Llanos, Lakisha Manning, Jayla Chambers, Megan Dolan | Maya Abraham, Abby Misch, Nauti'ka Robinson, Anaya Vil | DNS | 3:52.4256 | - | 98 |
