4 x 400 Fresh-Soph
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| PL | TEAM | ATHLETES | TIME | TIME | TIME | TIME | TIME | TIME | TIME |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Mainland | Marin Hawryluk, Taliyah Nixon, Autumn Demarest, Saraya Hinson | 5:35.16 | 4:54.00 | 5:52.80 | 4:01.08 | 6:16.32 | 4:24.60 | 6:04.56 |
| 2 | DeLand | Sydney Neira, Amy Nguyen, Sydney Bordeaux, Lilah Kersey | 6:01.99 | 5:04.19 | 5:49.82 | 5:46.78 | 3:57.27 | 6:20.24 | 3:39.02 |
| 3 | University (OC) | Lillie Pickens, Alea Smith, Shelsy Burgos Rosado, Sarahi Arroyo Canales | 7:03.98 | 5:33.84 | 4:30.41 | 5:53.87 | 4:37.09 | 4:03.71 | 4:13.72 |
| 4 | Lake Weir | 4:04.70 | 5:44.64 | 5:27.41 | 5:20.52 | 6:05.32 | 5:23.97 | 5:51.54 | |
| Father Lopez | Bianca Campos, Adelae Fulton, Marissa Stanberry, Frances Ginnochetti | DNS | DNS | DNS | DNS | DNS | DNS | DNS | |
| Olympia | Sandy Scott, Maddie Heald, Arlinda Roundtree, Korey Ardon | DNS | DNS | DNS | DNS | DNS | DNS | DNS |