4 x 400 Fresh-Soph
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| PL | TEAM | ATHLETES | TIME | TIME | TIME | TIME |
|---|---|---|---|---|---|---|
| 1 | Mainland | Marin Hawryluk, Taliyah Nixon, Autumn Demarest, Saraya Hinson | 5:55.74 | 5:41.04 | 4:54.00 | 3:40.50 |
| 2 | DeLand | Sydney Neira, Amy Nguyen, Sydney Bordeaux, Lilah Kersey | 5:19.40 | 6:17.20 | 5:04.19 | 5:43.74 |
| 3 | University (OC) | Lillie Pickens, Alea Smith, Shelsy Burgos Rosado, Sarahi Arroyo Canales | 4:07.05 | 7:00.64 | 5:33.84 | 4:47.11 |
| 4 | Lake Weir | 6:43.23 | 4:32.27 | 5:44.64 | 6:26.00 | |
| Father Lopez | Bianca Campos, Adelae Fulton, Marissa Stanberry, Frances Ginnochetti | DNS | DNS | DNS | DNS | |
| Olympia | Sandy Scott, Maddie Heald, Arlinda Roundtree, Korey Ardon | DNS | DNS | DNS | DNS |