| 1. | 13. | 2. | Cambridge Christian | Admiral Farragut | Admiral Farragut | Caroline Lehman, Moriah Friedman, Mary Ellen Eudaly, Elli Black | Kaela Williams, Sarah Rudolph, Emily Grandstaff, Sinkenesh Parker | Rebekah Haddad, Eliza Poe, Arianna Shaun, Ashylnn Turner | 9:26.122 | 9:58.7828 | 9:50.781 | 161017 | - | - |
| 2. | 15. | 7. | Oak Hall | Maclay | Bishop Snyder | Eliana Eisner, Sydney Miller, Gabriella Aulisio, Lauren Jones | Deborah Cox, Kaitlyn Frost, Lia Lynott, Emily Wheldon | Eliana Eisner, Sydney Miller, Gabriella Aulisio, Lauren Jones | 51:09:44.58 | 52:10:15.203 | 10:45.2724 | 88 | 148 | 5658 |
| 3. | 6. | 14. | Canterbury (FM) | Palmer Trinity | Circle Christian | Giana D'Altrui, Hayden Brands, Gabriella Santucci, Jessica Edwards | Rebekah Haddad, Eliza Poe, Arianna Shaun, Ashylnn Turner | Susan Koeppel, Lillian Koeppel, Mary Clayton Soto, Ella Porcher | 9:50.781 | 10:24.8629 | 9:26.122 | 5658 | 88 | - |
| 4. | 12. | 7. | Circle Christian | Providence | Northside Christian | Kaela Williams, Sarah Rudolph, Emily Grandstaff, Sinkenesh Parker | Kaela Williams, Sarah Rudolph, Emily Grandstaff, Sinkenesh Parker | Caroline Lehman, Moriah Friedman, Mary Ellen Eudaly, Elli Black | 59:09:53.514 | 59:09:53.514 | 52:10:15.203 | 510 | - | - |
| 5. | 12. | 1. | Indian Rocks Christian | Holy Trinity | Palmer Trinity | Ava Labrant, Kaci Anderson, Ayden Martin, Amelie Buras | Alexia Tine, Kayleigh Watts, Olivia Orobello, Bella Butler | Annika Lameyer, Trinity Hernandez, Sofia Rodriguez, Anna Richason | 9:53.77 | 23:10:38.502 | 38:10:45.38 | 148 | 88 | 3211 |
| 6. | 14. | 3. | Pensacola Christian | Palmer Trinity | Holy Trinity | Reagan Smith, Jaimee Tutton, RaeAnne Tutton, Brianna Sheppard | Ava Labrant, Kaci Anderson, Ayden Martin, Amelie Buras | Susan Koeppel, Lillian Koeppel, Mary Clayton Soto, Ella Porcher | 9:58.7828 | 9:53.77 | 49:10:21.90 | 034 | - | - |
| 7. | 12. | 16. | Maclay | Cambridge Christian | Holy Trinity | Susan Koeppel, Lillian Koeppel, Mary Clayton Soto, Ella Porcher | Giana D'Altrui, Hayden Brands, Gabriella Santucci, Jessica Edwards | Susan Koeppel, Lillian Koeppel, Mary Clayton Soto, Ella Porcher | 20:10:03.2392 | 9:58.7828 | 38:10:45.38 | 827 | 510 | 3211 |
| 8. | 3. | 9. | South Florida HEAT | South Florida HEAT | Indian Rocks Christian | Gracie Boesch, Paige Saville, Mia Kostelidis, Alyssa Benson | Alexia Tine, Kayleigh Watts, Olivia Orobello, Bella Butler | Giana D'Altrui, Hayden Brands, Gabriella Santucci, Jessica Edwards | 44:10:13.4237 | 10:24.8629 | 52:10:15.203 | 3211 | 510 | 161017 |
| 9. | 11. | 16. | Admiral Farragut | Circle Christian | Holy Trinity | Kelis Sims, Julia Mularoni, Elise Graham, Tekoyia Cheedy | Caroline Lehman, Moriah Friedman, Mary Ellen Eudaly, Elli Black | Rebekah Haddad, Eliza Poe, Arianna Shaun, Ashylnn Turner | 52:10:15.203 | 9:53.77 | 51:09:44.58 | - | - | 510 |
| 10. | 10. | 12. | Palmer Trinity | Holy Trinity | Oak Hall | Angelina Deleon, Gaby Dorta, Noelani Sadler, Daniella Perez | Deborah Cox, Kaitlyn Frost, Lia Lynott, Emily Wheldon | Rebekah Haddad, Eliza Poe, Arianna Shaun, Ashylnn Turner | 10:15.8023 | 23:10:38.502 | 59:09:53.514 | - | - | - |
| 11. | 13. | 16. | Bell Creek | Palmer Trinity | Northside Christian | Alexia Tine, Kayleigh Watts, Olivia Orobello, Bella Butler | Kaela Williams, Sarah Rudolph, Emily Grandstaff, Sinkenesh Parker | Annika Lameyer, Trinity Hernandez, Sofia Rodriguez, Anna Richason | 49:10:21.90 | 20:10:03.2392 | 9:53.77 | - | - | - |
| 12. | 3. | 11. | Providence | Oak Hall | Cambridge Christian | Lindy White, Gretchen Morgan, Sara Palmer, Kasey Davey | Giana D'Altrui, Hayden Brands, Gabriella Santucci, Jessica Edwards | Eliana Eisner, Sydney Miller, Gabriella Aulisio, Lauren Jones | 10:24.8629 | 9:58.7828 | 52:10:15.203 | - | - | 161017 |
| 13. | 9. | 15. | Bishop Snyder | Holy Trinity | Palmer Trinity | Deborah Cox, Kaitlyn Frost, Lia Lynott, Emily Wheldon | Kelis Sims, Julia Mularoni, Elise Graham, Tekoyia Cheedy | Ava Labrant, Kaci Anderson, Ayden Martin, Amelie Buras | 10:36.872 | 10:45.2724 | 10:45.2724 | - | - | 827 |
| 14. | 11. | 11. | Northside Christian | Maclay | Cambridge Christian | Annika Lameyer, Trinity Hernandez, Sofia Rodriguez, Anna Richason | Kelis Sims, Julia Mularoni, Elise Graham, Tekoyia Cheedy | Reagan Smith, Jaimee Tutton, RaeAnne Tutton, Brianna Sheppard | 23:10:38.502 | 49:10:21.90 | 23:10:38.502 | - | - | - |
| 15. | 5. | 16. | Tampa Bay HEAT | Indian Rocks Christian | Northside Christian | Rebekah Haddad, Eliza Poe, Arianna Shaun, Ashylnn Turner | Julia McNatt, Alinah Sarratt, Isabella Weiner, Sophia Weiner | Ava Labrant, Kaci Anderson, Ayden Martin, Amelie Buras | 10:45.2724 | 10:45.2724 | 9:53.77 | - | 148 | 5658 |
| 16. | 5. | 15. | Holy Trinity | Oak Hall | Pensacola Christian | Julia McNatt, Alinah Sarratt, Isabella Weiner, Sophia Weiner | Deborah Cox, Kaitlyn Frost, Lia Lynott, Emily Wheldon | Gracie Boesch, Paige Saville, Mia Kostelidis, Alyssa Benson | 38:10:45.38 | 10:36.872 | 44:10:13.4237 | - | 5658 | - |
