| 1. | 14. | Fort Myers | Sebring | Quin Roberts, Liam Holston, Jared Pocklington, Paul Kolsun | William Canales, Logan Burch, Ryan Mitchinson, Jake Jacoby | 8:01.883 | 19:08:35.162 | 10 | 2164 |
| 2. | 10. | Largo | Fort Myers | David Denkler, Jonathan Yacobi, Javarious Gordon, Alexander Staveley | Gabriel Munoz, David Stanley, Logan Gregory, Jaden Griffin | 8:05.70 | 19:09:15.941 | 889 | 6 |
| 3. | 8. | Estero | St. Petersburg | Isaiah Depina, Alexander Bagby, Avery Geerlings, Kolton Pickard | Ivan Gonzalez, Christian Cruz, Angel Nicolas, Elmer Mijangos-Figuer | 51:08:07.687 | 8:15.9472 | 6 | 625 |
| 4. | 2. | Dunbar | Mariner | Ogler Bartolon, Ber'Narjay Jackson, Daniel Ocampo, Ryan Livingston | Ogler Bartolon, Ber'Narjay Jackson, Daniel Ocampo, Ryan Livingston | 8:09.00 | 8:01.883 | 625 | - |
| 5. | 2. | Palmetto Ridge | Largo | Chris Amador, Nestor Hernandez, Jordan Velasquez, Saindeley Dorsainvil | Gabriel Munoz, David Stanley, Logan Gregory, Jaden Griffin | 8:15.9472 | 8:09.00 | 496 | - |
| 6. | 7. | Palmetto | Sebring | Ethan Martin, Jonathan Klepper, Miguel Sanchez, Randall Carter | Ogler Bartolon, Ber'Narjay Jackson, Daniel Ocampo, Ryan Livingston | 16:08:20.0837 | 8:09.00 | 73 | - |
| 7. | 14. | Sebring | Fort Myers | Andre Wilson, Hector Perez, Jacob Delong, Ian Velez | Kyle Thier, Wesley Walden, Isarb Fradin, Dominic Shannon | 8:21.0194 | 8:01.883 | 25 | 496 |
| 8. | 14. | Naples | Sebring | Hector Toro, Isaiah Barajas, Matthew Damouni, Andrew McLendon | Quin Roberts, Liam Holston, Jared Pocklington, Paul Kolsun | 35:08:25.258 | 16:08:20.0837 | 2164 | - |
| 9. | 15. | Mariner | Boca Ciega | Kyle Thier, Wesley Walden, Isarb Fradin, Dominic Shannon | Colby Bennett, Brendan Flavin, Kauhner Mault, Tyson Chumbley | 19:08:35.162 | 51:08:07.687 | - | - |
| 10. | 15. | Countryside | Largo | Gabriel Munoz, David Stanley, Logan Gregory, Jaden Griffin | Malachi McCloud, Kalomo Allen, Lawrence Budo, Kameron Krieger | 8:35.4991 | 19:09:15.941 | - | - |
| 11. | 13. | Charlotte | Fort Myers | Aidan Lowery, Tyler Olby, Simon Pettit, Christian Ortiz | Kendrick Wilson, Brayden Andringa, Jay Irby, Avery Reynolds | 6:08:39.205 | 51:08:07.687 | - | 889 |
| 12. | 7. | Cape Coral | Naples | William Canales, Logan Burch, Ryan Mitchinson, Jake Jacoby | Aidan Lowery, Tyler Olby, Simon Pettit, Christian Ortiz | 8:44.33 | 8:15.9472 | - | 889 |
| 13. | 6. | St. Petersburg | St. Petersburg | Kendrick Wilson, Brayden Andringa, Jay Irby, Avery Reynolds | Kendrick Wilson, Brayden Andringa, Jay Irby, Avery Reynolds | 8:45.15 | 8:09.00 | - | - |
| 14. | 15. | Immokalee | Charlotte | Ivan Gonzalez, Christian Cruz, Angel Nicolas, Elmer Mijangos-Figuer | Aidan Lowery, Tyler Olby, Simon Pettit, Christian Ortiz | 23:08:50.684 | 19:09:15.941 | - | 2164 |
| 15. | 11. | Boca Ciega | Palmetto | Malachi McCloud, Kalomo Allen, Lawrence Budo, Kameron Krieger | Quin Roberts, Liam Holston, Jared Pocklington, Paul Kolsun | 59:08:54.9012 | 59:08:54.9012 | - | 25 |
| 16. | 5. | Port Charlotte | Port Charlotte | Colby Bennett, Brendan Flavin, Kauhner Mault, Tyson Chumbley | Colby Bennett, Brendan Flavin, Kauhner Mault, Tyson Chumbley | 19:09:15.941 | 8:09.00 | - | 6 |
