| 1. | 3. | Estero | Estero | Isaiah Depina, Alexander Bagby, Kolton Pickard, Avery Geerlings | Isaiah Depina, Alexander Bagby, Kolton Pickard, Avery Geerlings | 2:08:26.297 | 9:16.61 | 104 | - |
| 2. | 6. | Lehigh | Dunbar | Evan Meyer, Willie McCray, Dennis Thomas, Anijah Holston | Justin Losapio, Ethan Cole, Michael Dow, Blake Wolters | 8:41.988 | 9:16.61 | 8 | 104 |
| 3. | 8. | Naples | Estero | Preston Gibson, Matthew Damouni, Andrew McLendon, Hector Toro | Jacob Fritz, Ryan Kinney, Angelo Rogato, Zac Phillips | 11:08:52.97 | 2:08:26.297 | 7666 | - |
| 4. | 8. | Mariner | Dunbar | Wesley Walden, Sebastien Soliman, Isarb Fradin, Kyle Thier | Rubens Michel, Nathan Horvath, Jonathan Grant, Rowen Fricke | 57:08:53.9123 | 8:41.988 | 7953 | 22 |
| 5. | 10. | Ida Baker | Mariner | Jacob Fritz, Ryan Kinney, Angelo Rogato, Zac Phillips | Isaiah Depina, Alexander Bagby, Kolton Pickard, Avery Geerlings | 8:59.17 | 17:09:33.339 | 245 | 104 |
| 6. | 7. | Dunbar | Riverdale | Olmar Bartolon, Joshua Dorestin, Jason Sanders, Fedrick Harris | Miguel Chavez, Ever Galeas, Juan Suarez, Dean King | 9:16.61 | 55:09:51.177 | 367 | 104 |
| 7. | 4. | Fort Myers | Bonita Springs | Mathew Weiler, Joseph Koraya, Brandon Bill, Jack Shea | Mathew Weiler, Joseph Koraya, Brandon Bill, Jack Shea | 18:09:19.940 | 8:59.17 | 22 | 245 |
| 8. | 1. | Riverdale | Ida Baker | Rubens Michel, Nathan Horvath, Jonathan Grant, Rowen Fricke | Jayden Klein, Chacour Thomas, Eric Perez, Connor Rees | 17:09:33.339 | 11:08:52.97 | 1 | 8 |
| 9. | 7. | East Lee County | Southwest Florida Christian | Miguel Chavez, Ever Galeas, Juan Suarez, Dean King | Justin Losapio, Ethan Cole, Michael Dow, Blake Wolters | 9:41.0177 | 2:08:26.297 | - | 7666 |
| 10. | 4. | Southwest Florida Christian | Naples | Justin Losapio, Ethan Cole, Michael Dow, Blake Wolters | Miguel Chavez, Ever Galeas, Juan Suarez, Dean King | 9:44.013 | 8:41.988 | - | - |
| 11. | 7. | Bonita Springs | Lehigh | Beckham Borgerink, Alex Sanchez, Benecio Carillo, Jules De Ron | Wesley Walden, Sebastien Soliman, Isarb Fradin, Kyle Thier | 55:09:51.177 | 55:09:51.177 | - | - |
| 12. | 1. | Gateway (Fort Myers) | East Lee County | Jayden Klein, Chacour Thomas, Eric Perez, Connor Rees | Rubens Michel, Nathan Horvath, Jonathan Grant, Rowen Fricke | 49:11:12.38 | 9:44.013 | - | 7666 |
