| 1. | 3. | Winter Springs | Oviedo | Grace Mason, Rylee Huet, Evie McIver, Caroline Wells | Maria Newman, Jessica Merly, Samantha Register, Sarah Messemer | 10:01.6565 | 35:10:54.27 | 6104 | 10 |
| 2. | 2. | Edgewater | Olympia | Ovella Ariail, Marina Brown, Decoursha Riley, Emma Krens | Abby Clark, Angelina Joseph, Yelena Tighiovart, Kayla McCarthy | 35:10:54.27 | 12:21.9721 | 82 | 373 |
| 3. | | Circle Christian | Cambridge Christian | Mia Carlson, Kelly Gregory, Gianna Kistner, Caroline Spivey | Grace Mason, Rylee Huet, Evie McIver, Caroline Wells | 3:10:55.725 | 10:01.6565 | 96 | 62 |
| 4. | 6. | Hagerty | Lake Brantley | Amanda Medina-Torres, Adriana Singh, Erin Rodd, Alena Pezzoli | Elena Caldwell, Ava Aseere, Thamia Romani, Ana Caldwell | 43:11:10.966 | 35:10:54.27 | 55 | - |
| 5. | 7. | Lake Minneola | Hagerty | Elena Caldwell, Ava Aseere, Thamia Romani, Ana Caldwell | Abby Clark, Angelina Joseph, Yelena Tighiovart, Kayla McCarthy | 12:11:24.589 | 51:12:33.81 | 47 | - |
| 6. | 5. | Oviedo | Edgewater | Jeanne Davis, Bailey Shams, Leola Broitman, Caroline Hickey | Karla Hormazabal, Jayline DeJesus, Yessmary Rodriguez, Alanis Soler | 11:24.8854 | 43:11:10.966 | 373 | 62 |
| 7. | 5. | Lyman | Master's Academy (Oviedo) | Madison Gordon, Cupo Abbigail, Jacqueline Maze, Maddy Rhodes | Madelyn Spadaro, Riley Spadaro, Kaiya Budwick, Lindie Mayer | 12:10.05 | 35:10:54.27 | 62 | - |
| 8. | 5. | Lake Brantley | Olympia | Abby Clark, Angelina Joseph, Yelena Tighiovart, Kayla McCarthy | Fiona Caceres, Jessica Gracia, kathryn Hey, Daniela Polakiewicz | 12:21.9721 | 51:12:33.81 | 10 | 62 |
| 9. | 5. | Master's Academy (Oviedo) | Oviedo | Maria Newman, Jessica Merly, Samantha Register, Sarah Messemer | Elena Caldwell, Ava Aseere, Thamia Romani, Ana Caldwell | 50:12:23.8139 | DNS | - | 373 |
| 10. | 8. | East Ridge | Master's Academy (Oviedo) | Karlie Saint-Lot, Lauren Mickens, Brittni Faber, Mackenzie Hill | Karla Hormazabal, Jayline DeJesus, Yessmary Rodriguez, Alanis Soler | 51:12:33.81 | 3:10:55.725 | - | - |
| | | Cambridge Christian | Colonial | Madelyn Spadaro, Riley Spadaro, Kaiya Budwick, Lindie Mayer | Karlie Saint-Lot, Lauren Mickens, Brittni Faber, Mackenzie Hill | DNS | DNS | - | - |
| | 4. | Olympia | Colonial | Fiona Caceres, Jessica Gracia, kathryn Hey, Daniela Polakiewicz | Maria Newman, Jessica Merly, Samantha Register, Sarah Messemer | DNS | 12:10.05 | - | - |
| | 4. | Colonial | Oviedo | Karla Hormazabal, Jayline DeJesus, Yessmary Rodriguez, Alanis Soler | Fiona Caceres, Jessica Gracia, kathryn Hey, Daniela Polakiewicz | DNS | 3:10:55.725 | - | 47 |
