| 1. | 4. | Canterbury (FM) | Lely | Hayden Brands, Michelle Barnes, Gabriella Santucci, Jessica Edwards | Savannah Taylor, Hannah Sangster, Trinity Castillo, Samantha Connar | 13:24.105 | 14:16.75 | 1095 | 81 |
| 2. | 16. | Cape Coral | Dunbar | Lucy Casto-Waters, Angely Rosas, Natalee Jones, Jennifer Jacoby | Brenna White, Caitlyn Sadofsky, Wendy Saynes Ruiz, Julia Vergo | 7:13:38.8749 | 15:32.46 | 81 | - |
| 3. | 8. | Naples | Community School (Naples) | Kathryn Lynch, Logan Straface, Jillian Dempsey, Madison McCormick | Kathryn Lynch, Logan Straface, Jillian Dempsey, Madison McCormick | 15:13:44.5932 | 16:47.9261 | 69 | - |
| 4. | 5. | Community School (Naples) | Mason Classical Academy | Loren Brown, Emilie Gesdorf, Maria O'Malley, Grace O'Malley | Melana Mourad, Isabella Dix, Samantha Nero, Margaret Lewis | 32:14:05.93 | 14:39.155 | 6352 | - |
| 5. | 15. | Estero | Mason Classical Academy | Madison Hood, Anna Bonini, Gracianne Goll, Annelisa Swiersz | Isabella Adams, Lauren Lappin, Mylee Van de Wouw, Mia Slaboch | 14:13.718 | 14:48.99 | 75412 | 21 |
| 6. | 16. | First Baptist (Naples) | Dunbar | Sloane Janopoulos, Aaliyah Smith, Kate Urbancic, Katelyn Beam | Alexandra Ditheridge, Sarah Nemeth, Ellie Poling, Maria De Clercq | 14:16.75 | 26:15:53.279 | 6384 | - |
| 7. | 10. | Village School | Community School (Naples) | Isabella Adams, Lauren Lappin, Mylee Van de Wouw, Mia Slaboch | Brenna White, Caitlyn Sadofsky, Wendy Saynes Ruiz, Julia Vergo | 14:18.77 | 15:13:44.5932 | 21 | - |
| 8. | 12. | Southwest Florida Christian | Dunbar | Sophia Dow, Alexandra Tartaglia, Abby Green, Emily Harris | Savannah Taylor, Hannah Sangster, Trinity Castillo, Samantha Connar | 14:39.155 | 38:15:11.5235 | 81 | - |
| 9. | 4. | North Fort Myers | Community School (Naples) | Savannah Taylor, Hannah Sangster, Trinity Castillo, Samantha Connar | Sloane Janopoulos, Aaliyah Smith, Kate Urbancic, Katelyn Beam | 10:14:39.184 | 16:28.199 | - | - |
| 10. | 14. | Lely | Marco Island Academy | Alexandra Ditheridge, Sarah Nemeth, Ellie Poling, Maria De Clercq | noura naami, Kaitlyn Boyette, Khadija Doha, Azlin Thompson | 14:48.99 | 15:41.1945 | - | 69 |
| 11. | 2. | Barron Collier | North Fort Myers | Joan Khalenya, Natalie Bongirno, Emily Rypina, Lauryn Jackson | Lineriya McGriff, Asiah Douglas, Hailey Romero, Sabrina Clermont | 38:15:11.5235 | 15:32.46 | - | 1095 |
| 12. | 2. | South Plantation | North Fort Myers | noura naami, Kaitlyn Boyette, Khadija Doha, Azlin Thompson | noura naami, Kaitlyn Boyette, Khadija Doha, Azlin Thompson | 15:32.46 | 14:39.155 | - | - |
| 13. | 14. | Marco Island Academy | Riverdale | Brenna White, Caitlyn Sadofsky, Wendy Saynes Ruiz, Julia Vergo | Melana Mourad, Isabella Dix, Samantha Nero, Margaret Lewis | 15:41.1945 | 15:32.46 | - | 81 |
| 14. | 15. | Riverdale | Naples | Logan Geren, Jasmine Vasquez, Elizabeth Morgan, Yaky Delorey | Sloane Janopoulos, Aaliyah Smith, Kate Urbancic, Katelyn Beam | 26:15:53.279 | 15:13:44.5932 | - | - |
| 15. | 17. | Mason Classical Academy | North Fort Myers | Melana Mourad, Isabella Dix, Samantha Nero, Margaret Lewis | Kathryn Lynch, Logan Straface, Jillian Dempsey, Madison McCormick | 16:28.199 | 14:18.77 | - | 1095 |
| 16. | 5. | Dunbar | Mason Classical Academy | Lineriya McGriff, Asiah Douglas, Hailey Romero, Sabrina Clermont | Vanessa Spinelli, Sabrina Pierce, Alana Brooke, Jordyn Puthoff | 44:16:30.56 | 26:15:53.279 | - | 69 |
| 17. | 7. | Gulf Coast | Canterbury (FM) | Vanessa Spinelli, Sabrina Pierce, Alana Brooke, Jordyn Puthoff | Lineriya McGriff, Asiah Douglas, Hailey Romero, Sabrina Clermont | 16:47.9261 | 38:15:11.5235 | - | - |
