| 1. | | Winter Springs | Master's Academy (Oviedo) | Elayna Bahr, Naomi Flowers, Mackenzie Czurak, Caroline Wells | Jaqueline Christiansen, D'arri Zellner, Tania Bolden, Madelyn Bronson | 11:59.675 | DNS |
| 2. | 5. | Lake Minneola | Master's Academy (Oviedo) | Elena Caldwell, Snejana Faryga, Ellie Kunze, Ava Aseere | Juliet St Angelo, Brianna Crawford, Emily Legler , Keegan Brace | 13:32.92 | 11:59.675 |
| 3. | 5. | Master's Academy (Oviedo) | Olympia | Ryleigh Wilson, Lilly Jacobus, Ava Wyant, Maria Newman | Rachel Wheatley, Elise Butler, Victoria Valdez, Olivia Niewald | 13:39.4542 | DNS |
| 4. | 1. | Oviedo | Mainland | Ryleigh Holcomb, Joyce Davis, Jeanne Davis, Sophia Johnson | kathryn Hey, Emma Dahlgren, JoAnn Almonte, Taylor Figueras | 26:13:40.9981 | 24:15:55.83 |
| 5. | | Lake Howell | Lake Howell | Juliet St Angelo, Brianna Crawford, Emily Legler , Keegan Brace | Elena Caldwell, Snejana Faryga, Ellie Kunze, Ava Aseere | 41:13:56.826 | 13:32.92 |
| 6. | | Mainland | Lake Mary | Jaqueline Christiansen, D'arri Zellner, Tania Bolden, Madelyn Bronson | Jaqueline Christiansen, D'arri Zellner, Tania Bolden, Madelyn Bronson | 15:28.73 | 41:13:56.826 |
| 7. | | Lake Mary | Lake Mary | Abigail Davis, Cymiah Bradley, Caitlin Bennett, Mackenzie Vanhees | Jaqueline Christiansen, D'arri Zellner, Tania Bolden, Madelyn Bronson | 24:15:55.83 | 15:28.73 |
| | | Spruce Creek | Master's Academy (Oviedo) | Rachel Wheatley, Elise Butler, Victoria Valdez, Olivia Niewald | Bailey Stokes, Emma Krens, Neah Montgomery, Ovella Ariail | DNS | 41:13:56.826 |
| | 5. | Olympia | Olympia | kathryn Hey, Emma Dahlgren, JoAnn Almonte, Taylor Figueras | Juliet St Angelo, Brianna Crawford, Emily Legler , Keegan Brace | DNS | 11:59.675 |
| | 4. | Edgewater | Mainland | Bailey Stokes, Emma Krens, Neah Montgomery, Ovella Ariail | Abigail Davis, Cymiah Bradley, Caitlin Bennett, Mackenzie Vanhees | DNS | 13:39.4542 |
