| 1. | 2. | Vero Beach | Vero Beach | Niyah Yasin, Jamya Trusty, Juliet Russell, Jada Rolle | Nyzeria Lewis, Ashley Buchanan, Katriel Budwah, Eltaneia Francois | 4:23.02 | DNS | 81047 | 25 |
| 2. | 5. | Lincoln Park | Fort Pierce Westwood | Chloe Wright, Ariel Major, Melissa Gale, Amber Clark | Alyssia Vecchione, Keelin Johnson, Ava DePeri, Amiyah Lee | 4:29.1326 | 4:54.8075 | 80 | - |
| 3. | 5. | Martin County | Jensen Beach | Elizabeth Chu, Gina Fuca, Anna Tullidge, Sophia Poirier | Nyzeria Lewis, Ashley Buchanan, Katriel Budwah, Eltaneia Francois | 4:42.02 | 22:05:04.6726 | 967 | - |
| 4. | 4. | Fort Pierce Westwood | Vero Beach | Raziyah Stewart, Sofia Tovar, Trinity Cook, Aniya Porter | Elizabeth Chu, Gina Fuca, Anna Tullidge, Sophia Poirier | 6:04:51.345 | 22:05:04.6726 | 25 | 25 |
| 5. | 6. | Sebastian River | Port St. Lucie | Alyssia Vecchione, Keelin Johnson, Ava DePeri, Amiyah Lee | Lillian Gonda, Kate Marsh, Maddie Meekins, Gianna Panarelli | 4:54.8075 | 22:05:04.6726 | 54 | 54 |
| 6. | 1. | Fort Pierce Central | Jensen Beach | Nyzeria Lewis, Ashley Buchanan, Katriel Budwah, Eltaneia Francois | Chloe Wright, Ariel Major, Melissa Gale, Amber Clark | 54:05:00.9469 | 4:54.8075 | 5236 | 54 |
| 7. | | Port St. Lucie | Vero Beach | La'Nia Rogers, Ruth Jeanty, Aliyah Haque, Jahzarra Laurent | Nyzeria Lewis, Ashley Buchanan, Katriel Budwah, Eltaneia Francois | 22:05:04.6726 | 22:05:04.6726 | 5271 | - |
| | 3. | Jensen Beach | Fort Pierce Central | Lillian Gonda, Kate Marsh, Maddie Meekins, Gianna Panarelli | Raziyah Stewart, Sofia Tovar, Trinity Cook, Aniya Porter | DNS | 22:05:04.6726 | - | 967 |
