| 1. | 16. | Dr. Phillips | Winter Park | Jasmine Little, Roxanne Deisseroth, Teaghan Connolly, Hannah Claytor | Alanis Soler, Yessmary Rodriguez, Paula Prek, Araceliz Rosario | 10:09.86 | DNS | 81052 | 44 |
| 2. | 3. | Timber Creek | St. Cloud | Vivian Byrnes, Carly Holadia, Sierra Orie, Amber Schulz | Olivia Lamontagne, Janiyah Hall, Karina Vega | 50:10:17.33 | 0:10:32.88 | 80 | - |
| 3. | 14. | East River | Melbourne | Olivia Lamontagne, Janiyah Hall, Karina Vega | Olivia Lamontagne, Janiyah Hall, Karina Vega | 10:22.8405 | 46:11:17.46 | 6 | 6 |
| 4. | 4. | George Jenkins | South Fork | Kaleigh Malick, Anna Sentner, Viktorya Manno, Alicia Ruiz | Melanie Estes, Avery Rouhoff, Jasmine Camacho, Carolyn Kelley | 0:10:32.88 | 51:12:55.754 | 75 | 6 |
| 5. | 1. | Winter Park | Dr. Phillips | Alisa Valencia, Peyton Damewood , Julia Reitz, Olivia Ranno | Lauren Barnes, Kayla Hall, Florence Onyiuke, Allison Stromberg | 10:51.675 | 14:11:11.48 | 44 | 0427 |
| 6. | 10. | Viera | Colonial | Melanie Estes, Avery Rouhoff, Jasmine Camacho, Carolyn Kelley | Brooke Hawkins, Madeline Weiler, Taylor Sesta, Isabella Nogueira | 20:11:01.02 | DNS | 1338 | 75 |
| 7. | 13. | South Fork | Melbourne | Brooke Hawkins, Madeline Weiler, Taylor Sesta, Isabella Nogueira | Jasmine Little, Roxanne Deisseroth, Teaghan Connolly, Hannah Claytor | 14:11:11.48 | 11:19.7288 | 0427 | - |
| 8. | 15. | Colonial | Ridge Community | Alanis Soler, Yessmary Rodriguez, Paula Prek, Araceliz Rosario | Melanie Estes, Avery Rouhoff, Jasmine Camacho, Carolyn Kelley | 11:16.750 | 10:51.675 | 15 | - |
| 9. | 8. | St. Cloud | Viera | Gabriela Lugo Pardo, Tiana Rivera, Brenna Bush, Kenzie Brantley | Brooke Hawkins, Madeline Weiler, Taylor Sesta, Isabella Nogueira | 46:11:17.46 | 14:11:11.48 | - | - |
| 10. | 2. | City of Life Christian | George Jenkins | Ashanti Ramiez, Kaylee Harkema, Isabella Souza, Kalena Ramirez | Jetzymar Rivera, Melanie Arriola, Kelliana Mack, Aysia Stokes | 11:19.7288 | 10:09.86 | - | - |
| 11. | 7. | Osceola (Kissimmee) | Colonial | Jetzymar Rivera, Melanie Arriola, Kelliana Mack, Aysia Stokes | Gabriela Lugo Pardo, Tiana Rivera, Brenna Bush, Kenzie Brantley | 11:30.55 | 11:11:44.14 | - | - |
| 12. | 13. | Ridge Community | Liberty | Arely Cruz, Karina Pagan Ortiz, Tania Pagan Ortiz, Sade' Ivey | Angie Sylfain, Demi Hilton, Onnia Dalmida, Analise Beecher | 31:11:33.051 | 11:19.7288 | - | - |
| 13. | 3. | Cypress Creek | Cypress Creek | Ariana Euceda, Julie Santos, Sophia Sundin, Nyasia Rivera | Jetzymar Rivera, Melanie Arriola, Kelliana Mack, Aysia Stokes | 11:11:44.14 | 50:10:17.33 | - | 44 |
| 14. | 4. | Lake Nona | South Fork | Valerie Hoyos, Lillian Nguyen, Noel Wiltz, Emily Thompson | Gabriela Lugo Pardo, Tiana Rivera, Brenna Bush, Kenzie Brantley | 11:47.822 | 11:11:44.14 | - | - |
| 15. | 5. | Melbourne | City of Life Christian | Hannah Phillips, Stella Chambers, Piper Stephens, Katelyn Apotsos | Angie Sylfain, Demi Hilton, Onnia Dalmida, Analise Beecher | 51:12:55.754 | 20:11:01.02 | - | 15 |
| 16. | 14. | Liberty | Melbourne | Angie Sylfain, Demi Hilton, Onnia Dalmida, Analise Beecher | Gabriela Lugo Pardo, Tiana Rivera, Brenna Bush, Kenzie Brantley | 45:13:12.6942 | 10:09.86 | - | 80 |
| | 2. | Seminole (Sanford) | Winter Park | Lauren Barnes, Kayla Hall, Florence Onyiuke, Allison Stromberg | Melanie Estes, Avery Rouhoff, Jasmine Camacho, Carolyn Kelley | DNS | 10:51.675 | - | 6 |
