| 1. | | Mainland | New Smyrna Beach | Malcolm Reed, Lindon Cooper, Besean Ford, Arnold Collins | Chris Hill, Kaden Jung, Ryan Alderman, Erie Peeples | 45:03:22.2506 | 3:39.65 | 4210 | 85 |
| 2. | 13. | Sebring | Vanguard (Ocala) | Jaheim Sanders, Chase Doty, Shareal Taylor, Jabari Knighten | Jacob Hindman, Jacob Unger, Jeffery Chamberlin, Nabil Koney-Laryea | 3:23.0932 | 3:27.834 | 8981 | 3444 |
| 3. | 4. | Mitchell | Wesley Chapel | Jacob Hindman, Jacob Unger, Jeffery Chamberlin, Nabil Koney-Laryea | Okechukwu Nkwocha, Sean Murray, James Phillips, Vance Ruiz | 3:27.834 | 26:03:29.9825 | 61 | - |
| 4. | 1. | Harmony | Matanzas | Kareem Dinzey, Joshua Grimes, Gibson Gaston, Christian Taylor | Jontavius Anderson, Alex Hayden, Jeremy Young, Brandon Hitch | 3:27.839 | 3:40.62 | 85 | 3444 |
| 5. | 1. | Vanguard (Ocala) | Poinciana | Jordan Travieso, Gio Rodriguez, Jaywaun Nubin, Montre'ye Edwards | Jason Abbott, DeRel Morris, Dustin Newton, Eric Aquino | 26:03:29.9825 | 3:30.122 | 3444 | 85 |
| 6. | 11. | Winter Haven | New Smyrna Beach | Dejuan Henderson, D'Angelo Sapp, Joaquin Jackson, Mario Tullis | Manuel Marrero-Introini, Jaden Cofield, Jason Ryder, Denzel Whisonant | 3:30.122 | DQ | 3 | - |
| 7. | 15. | East River | Winter Haven | Jason Abbott, DeRel Morris, Dustin Newton, Eric Aquino | Solomon Davis, Isaac Taveras, Cade Whitfield, Ludcheel Colas | 48:03:33.05 | 17:03:39.894 | 2262 | - |
| 8. | 3. | Wesley Chapel | Forest | Jontavius Anderson, Alex Hayden, Jeremy Young, Brandon Hitch | Mason Gollwitzer, Heddwyn Bulley, Noah Frazier, Jackson Cole | 3:33.47 | 3:33.47 | 871 | - |
| 9. | 2. | Matanzas | Leesburg | Kevin Connelly, Maxwell Hornstein, Jayce Friendly, Jonathan Rodriguez | Solomon Davis, Isaac Taveras, Cade Whitfield, Ludcheel Colas | 5:03:39.31 | 5:03:39.31 | - | 3444 |
| 10. | 9. | Sunlake | Wesley Chapel | Solomon Davis, Isaac Taveras, Cade Whitfield, Ludcheel Colas | Solomon Davis, Isaac Taveras, Cade Whitfield, Ludcheel Colas | 3:39.65 | 3:40.62 | - | - |
| 11. | 11. | Gainesville | Lecanto | Mason Gollwitzer, Heddwyn Bulley, Noah Frazier, Jackson Cole | Jason Abbott, DeRel Morris, Dustin Newton, Eric Aquino | 17:03:39.894 | 3:39.65 | - | - |
| 12. | 12. | Poinciana | Harmony | Manuel Marrero-Introini, Jaden Cofield, Jason Ryder, Denzel Whisonant | Jason Abbott, DeRel Morris, Dustin Newton, Eric Aquino | 3:40.62 | 3:44.18 | - | 871 |
| 13. | 12. | Forest | East River | Justin Kernan, McCoughan Peacock, Nicholas Kernan, Ashton Deriso | Okechukwu Nkwocha, Sean Murray, James Phillips, Vance Ruiz | 52:03:41.8706 | 3:44.18 | - | - |
| 14. | 10. | Leesburg | Gainesville | Tylan Christopher, Micah Forsythe, Brock Money, William Troupe | Justin Kernan, McCoughan Peacock, Nicholas Kernan, Ashton Deriso | 3:42.71 | 45:03:22.2506 | - | 4210 |
| 15. | 12. | New Smyrna Beach | Poinciana | Chris Hill, Kaden Jung, Ryan Alderman, Erie Peeples | Jontavius Anderson, Alex Hayden, Jeremy Young, Brandon Hitch | 3:44.18 | 3:40.62 | - | - |
| | 11. | Lecanto | Wesley Chapel | Okechukwu Nkwocha, Sean Murray, James Phillips, Vance Ruiz | Kevin Connelly, Maxwell Hornstein, Jayce Friendly, Jonathan Rodriguez | DQ | 3:30.122 | - | - |
