| 1. | 3. | 6. | Venice | Venice | Osceola (Seminole) | Juliana Courville, Hannah Robertson, Rachel Rose, Gabby La Rock | Isabella Reyes, Emmalee Lowe, Celine Fleurilus, Nicholette Moss | Ciarra Durr, Janeaja Wright, Kourtney Warren, Leah Daniel | 4:02.129 | 34:04:20.7035 | 24:04:26.81 | 3510 | 66431 | - |
| 2. | 12. | 1. | Hillsborough | Naples | Venice | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | Juliana Courville, Hannah Robertson, Rachel Rose, Gabby La Rock | Francoise Fabien, Emma Aristizibal, Joan Khalenya, Aaliyah Seaborn | 46:04:08.07 | 46:04:33.48 | 4:20.7509 | 5842 | 972 | 5842 |
| 3. | 16. | 7. | Naples | Naples | Fort Myers | Olivia Ponton, Zoe Dantonio, Merlica Faustin, Marina Turchetta | Francoise Fabien, Emma Aristizibal, Joan Khalenya, Aaliyah Seaborn | Emille Hauser, Alanna Hause, Ashley Easterly, Jasmin Teran | 5:04:11.11 | 4:28.5139 | 4:17.48 | 686 | - | - |
| 4. | 8. | 14. | Fort Myers | Ida Baker | Braden River | Megan Denny, Katrina Lee, Elizabeth Imbody, Gabriella Navas | Brianna Alderman, Nithya Chummar, Asha Armstrong, Sofia Rivera | Erika Sodeika, Sage Fitzgerald, Olivia Lamontagne, Shainelle Joseph | 18:04:12.826 | 4:15.6793 | 40:04:14.1381 | 5 | 5842 | 686 |
| 5. | 8. | 16. | Golden Gate | Tampa Bay Tech | Barron Collier | Chelna Francois, Jasmine Dera, Rose Jean, Darlene Thermidor | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | Brianna Alderman, Nithya Chummar, Asha Armstrong, Sofia Rivera | 40:04:14.1381 | 12:04:15.9221 | 4:17.918 | 66431 | 972 | 34 |
| 6. | 14. | 2. | Braden River | East Lake | East Lake | Rose Ferla Philogene, Kenzie Johnson, Grace Marston, Hailey Marston | Carly Bradley, Nicole Fels, Madisen Paster, Olivia Mackenzie | Chelna Francois, Jasmine Dera, Rose Jean, Darlene Thermidor | 4:15.6793 | 4:28.5139 | 4:17.918 | 34 | - | 66431 |
| 7. | 5. | 5. | Tampa Bay Tech | North Fort Myers | Barron Collier | Natasha Kerr, Ashliona Martin, Kailah Gosa, Sharielle Ransom | Natasha Kerr, Ashliona Martin, Kailah Gosa, Sharielle Ransom | Riley Fritz, Janelle Llenas, Ashley Watson, Dashnie Dervil | 12:04:15.9221 | 38:04:34.141 | 24:04:26.81 | 972 | - | 5842 |
| 8. | 6. | 10. | Barron Collier | Gulf Coast | Fort Myers | Francoise Fabien, Emma Aristizibal, Joan Khalenya, Aaliyah Seaborn | Francoise Fabien, Emma Aristizibal, Joan Khalenya, Aaliyah Seaborn | Francoise Fabien, Emma Aristizibal, Joan Khalenya, Aaliyah Seaborn | 31:04:17.322 | 4:17.48 | 38:04:34.141 | 951 | - | - |
| 9. | 16. | 16. | Ida Baker | Port Charlotte | Venice | Riley Fritz, Janelle Llenas, Ashley Watson, Dashnie Dervil | Emille Hauser, Alanna Hause, Ashley Easterly, Jasmin Teran | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | 4:17.48 | 4:28.5139 | 40:04:14.1381 | - | - | - |
| 10. | 15. | 1. | Port Charlotte | Port Charlotte | Tampa Bay Tech | Isabella Reyes, Emmalee Lowe, Celine Fleurilus, Nicholette Moss | Brianna Alderman, Nithya Chummar, Asha Armstrong, Sofia Rivera | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | 4:17.918 | 46:04:33.48 | 46:04:08.07 | - | - | 5842 |
| 11. | 7. | 8. | King | Brandon | Hillsborough | Ciarra Durr, Janeaja Wright, Kourtney Warren, Leah Daniel | Emille Hauser, Alanna Hause, Ashley Easterly, Jasmin Teran | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | 34:04:20.7035 | 18:04:12.826 | 4:28.5139 | - | - | - |
| 12. | 5. | 14. | Gulf Coast | Barron Collier | Gulf Coast | Erika Sodeika, Sage Fitzgerald, Olivia Lamontagne, Shainelle Joseph | Olivia Ponton, Zoe Dantonio, Merlica Faustin, Marina Turchetta | Olivia Ponton, Zoe Dantonio, Merlica Faustin, Marina Turchetta | 4:20.7509 | 46:04:33.48 | 38:04:34.141 | - | - | 972 |
| 13. | 5. | 7. | East Lake | Golden Gate | Braden River | Carly Bradley, Nicole Fels, Madisen Paster, Olivia Mackenzie | Ciarra Durr, Janeaja Wright, Kourtney Warren, Leah Daniel | Isabella Reyes, Emmalee Lowe, Celine Fleurilus, Nicholette Moss | 24:04:26.81 | 12:04:15.9221 | 4:20.7509 | - | 5842 | - |
| 14. | 7. | 9. | North Fort Myers | Barron Collier | Venice | Emille Hauser, Alanna Hause, Ashley Easterly, Jasmin Teran | Rose Ferla Philogene, Kenzie Johnson, Grace Marston, Hailey Marston | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | 4:28.5139 | 34:04:20.7035 | 4:20.7509 | - | 972 | 972 |
| 15. | 14. | 9. | Brandon | Golden Gate | Brandon | Brianna Alderman, Nithya Chummar, Asha Armstrong, Sofia Rivera | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | 46:04:33.48 | 38:04:34.141 | 12:04:15.9221 | - | - | - |
| 16. | 14. | 16. | Osceola (Seminole) | Osceola (Seminole) | Brandon | Meghan Gregoire, Ava Reichle, Amanda Evans, Chigozie Ikegwu | Meghan Gregoire, Ava Reichle, Amanda Evans, Chigozie Ikegwu | Shawna Woodall, Alexis Woodall, Lycia Zavala, Justyce Murphy | 38:04:34.141 | 4:20.7509 | 4:17.48 | - | 66431 | 5 |
