| 1. | 3. | Lakewood Ranch | Lakewood Ranch | Sydney Granack, Emma Donato, Madeline McNinch, Ava Klein | Sydney Granack, Emma Donato, Madeline McNinch, Ava Klein | 46:04:06.6281 | 4:33.76 | 71091 | 68 |
| 2. | 3. | North Port | North Port | Taylor Gray, Taylor Cooper, kylah Buckle, Sarah Mendez | Taylor Gray, Taylor Cooper, kylah Buckle, Sarah Mendez | 4:13.07 | 4:13.07 | 68 | 02334 |
| 3. | 5. | Sarasota | Lakewood Ranch | Capri Procopio, Isabelle Jester, Carolina Titus, Janel Mitchell | Sydney Granack, Emma Donato, Madeline McNinch, Ava Klein | 4:04:18.010 | 46:04:06.6281 | 69 | 71091 |
| 4. | 2. | St. Petersburg | St. Petersburg | Ava Abrahams, Alexa Sweigart, Janaya Davis-Gainer, Makenna Myrick | Sydney Granack, Emma Donato, Madeline McNinch, Ava Klein | 30:04:29.9557 | 4:13.07 | 62510 | 71091 |
| 5. | 5. | Seminole | St. Petersburg | Angelina Haywood, Katherine Repka, Meagan Bradley, Hailey Smith | Katlyn Patterson, Nicole Jackson, Kyla Hargrave, Haley Wargo | 4:33.76 | 4:33.76 | 304 | 304 |
| 6. | 6. | Countryside | Sarasota | Katlyn Patterson, Nicole Jackson, Kyla Hargrave, Haley Wargo | Sydney Granack, Emma Donato, Madeline McNinch, Ava Klein | 5:11.95 | 4:13.07 | 02334 | 62510 |
