| 1. | 6. | Winter Park | Colonial | Jani Day, Jazlin Dailey, Clarisa Groff, Gabrielle Johnson | Linette Fernandez, Dejah Ojeda, Scharmensey Fine, TreazuâÂÂre Sims | 4:04.232 | 4:08.875 | 5210 | 1547 |
| 2. | | Dr. Phillips | Winter Park | Zoe Alexander, Rayanna Mathis, Jarvia Carr, Domanique Douglas | Bensdjadini Michel, Deborah Georges, Paquisha Eutsey, Naika Metellus | 4:07.161 | 4:04.232 | 844 | - |
| 3. | | Timber Creek | Freedom (Orlando) | Alana Wolfberg, Zniah Hutchinson, Julia Rodriguez, Savannah Henderson | Ruth Joseph, Camila Palomino, Yenilitza Roman, Jessica Jeffrey | 4:08.875 | 4:08.875 | 6 | 6 |
| 4. | 1. | Lake Nona | University (Orlando) | Sydni Brown, Justyn Smith, Marlia Marston, Xiomara Gill | Zoe Alexander, Rayanna Mathis, Jarvia Carr, Domanique Douglas | 4:13.138 | 47:04:24.393 | 1547 | 5210 |
| 5. | | Freedom (Orlando) | Timber Creek | Ruth Joseph, Camila Palomino, Yenilitza Roman, Jessica Jeffrey | Kristin Deshotel, Kaitlin Deshotel, Bryanna King, Leyshka Arroyo | 47:04:24.393 | DNS | 46 | 02211 |
| 6. | 5. | Colonial | Boone | Kristin Deshotel, Kaitlin Deshotel, Bryanna King, Leyshka Arroyo | Alana Wolfberg, Zniah Hutchinson, Julia Rodriguez, Savannah Henderson | 4:24.78 | 4:08.875 | 39 | 1547 |
| 7. | 4. | Boone | Dr. Phillips | Michelle Gunn, Sarah Strange, Reathasia Outler, Carolyn Brown | Jani Day, Jazlin Dailey, Clarisa Groff, Gabrielle Johnson | 59:04:46.819 | DNS | 02211 | - |
| | 5. | Oak Ridge | University (Orlando) | Bensdjadini Michel, Deborah Georges, Paquisha Eutsey, Naika Metellus | Michelle Gunn, Sarah Strange, Reathasia Outler, Carolyn Brown | DNS | DNS | - | 46 |
| | 2. | University (Orlando) | Colonial | Linette Fernandez, Dejah Ojeda, Scharmensey Fine, TreazuâÂÂre Sims | Bensdjadini Michel, Deborah Georges, Paquisha Eutsey, Naika Metellus | DNS | 4:24.78 | - | 02211 |
