| 1. | 4. | Sarasota | Sarasota | Marissa Kempf, Carolina Titus, Isabelle Jester, Janel Mitchell | Megan Graser, Caroline Rice, Emma Rose, Blake Petrick | 41:09:51.760 | 11:14.6955 |
| 2. | 2. | Creekside | P. K. Yonge | Megan Graser, Caroline Rice, Emma Rose, Blake Petrick | Marissa Kempf, Carolina Titus, Isabelle Jester, Janel Mitchell | 10:13.05 | 10:35.76 |
| 3. | 8. | Buchholz | Bartram Trail | Allison Drummond, Caitlin Rowe, Glenna Li, Taylor Veatch | Laci Herron, Riley Blackwelder, Jocelyn Riley, Anastasia Fernandez Smith | 56:10:23.925 | 46:11:17.65 |
| 4. | 8. | Father Lopez | Raines | Alexis Dawson, Claire McDonnell, Mary Peck, Victoria Woods | Selket Reid, Krishanti Pease, Jakkia Sams, Semaj Johnson | 10:35.76 | 10:35.76 |
| 5. | 5. | St. Joseph | Bartram Trail | Katie Whitehouse, Isabella Goodwin, Julianna Binder, Leticia Martinez | Ella Santilli, Sydney Olsen, Elizabeth Rodriguez, Brianna Mitchell | 49:11:08.44 | 36:11:21.8739 |
| 6. | 8. | Raines | Raines | Selket Reid, Krishanti Pease, Jakkia Sams, Semaj Johnson | Selket Reid, Krishanti Pease, Jakkia Sams, Semaj Johnson | 11:14.6955 | 41:09:51.760 |
| 7. | 6. | P. K. Yonge | Matanzas | Laci Herron, Riley Blackwelder, Jocelyn Riley, Anastasia Fernandez Smith | Selket Reid, Krishanti Pease, Jakkia Sams, Semaj Johnson | 46:11:17.65 | 43:12:04.84 |
| 8. | 1. | Bartram Trail | Buchholz | Elizabeth Camp, Maggie Solloway, Logan Henley, Alana Greene | Marissa Kempf, Carolina Titus, Isabelle Jester, Janel Mitchell | 36:11:21.8739 | 36:11:21.8739 |
| 9. | 6. | Matanzas | Father Lopez | Ella Santilli, Sydney Olsen, Elizabeth Rodriguez, Brianna Mitchell | Marissa Kempf, Carolina Titus, Isabelle Jester, Janel Mitchell | 43:12:04.84 | 11:14.6955 |
