| 1. | 4. | Sunlake | Tampa Bay Tech | Nia Johnson, Jaymonda Calmes, Gianna Levy, Hannah Kilmer | Hannah Brooks, Lilou Stegeman, Giovanna Mallicote Sallab, Zayne Jack | 049.88 | 249.902 | 0105 | 0105 |
| 2. | 3. | Tampa Bay Tech | Palm Harbor University | Ashliona Martin, Natasha Kerr, Amsy Takeli, Kailah Gosa | Jarlenys Cordoba, Kya Chen, Aliyah Torres, Maya Ashley | 249.902 | 51.55 | 87820 | - |
| 3. | 2. | Alonso | George Jenkins | Jarlenys Cordoba, Kya Chen, Aliyah Torres, Maya Ashley | Kershon Speights, Starsheena Calixte, Anayah Elston, I'Asia Wilson | 6551.1150 | DNF | 76 | 40538 |
| 4. | 5. | Land O'Lakes | Sunlake | Sawyer Bush, Avery Wild, Kylie Denney, Taylor Denney | Allison Fleming, Shaina Finkel, Christy Faddoul, Kat Fleming | 51.55 | 51.8522 | 40538 | 76 |
| 5. | 5. | Wiregrass Ranch | Lakeland | Allison Fleming, Shaina Finkel, Christy Faddoul, Kat Fleming | Victoria Rodriquez, Skylin Spencer, Mckensie Myers, Grace Christiansen | 51.8135 | 249.902 | 084 | 0105 |
| 6. | 4. | George Steinbrenner | Land O'Lakes | Hannah Brooks, Lilou Stegeman, Giovanna Mallicote Sallab, Zayne Jack | Hannah Brooks, Lilou Stegeman, Giovanna Mallicote Sallab, Zayne Jack | 51.8522 | 52.483 | 693 | 2184 |
| 7. | 2. | Palm Harbor University | George Jenkins | Olivia Whiddon, Reese Small, Kylie Cimillo, Dilynn Blair | Kershon Speights, Starsheena Calixte, Anayah Elston, I'Asia Wilson | 52.483 | 049.88 | 92 | 87820 |
| 8. | 6. | Mitchell | Mitchell | Victoria Rodriquez, Skylin Spencer, Mckensie Myers, Grace Christiansen | Sawyer Bush, Avery Wild, Kylie Denney, Taylor Denney | 28:01:01.80 | 52.483 | 2184 | 92 |
| | 7. | George Jenkins | Wiregrass Ranch | Suzan Wilson Cole, Kelsie Divvens, Zhane Alexander, Heaven Barr | Olivia Whiddon, Reese Small, Kylie Cimillo, Dilynn Blair | DQ | 52.483 | - | 693 |
| | 6. | Lakeland | Lakeland | Kershon Speights, Starsheena Calixte, Anayah Elston, I'Asia Wilson | Allison Fleming, Shaina Finkel, Christy Faddoul, Kat Fleming | DNF | 51.8135 | - | 92 |
