| 1. | 3. | Springstead | Weeki Wachee | Ivey Francher, Alyssa Williams, Yanializ Bonnelly, Kylie Kanaar | | 10:26.119 | 12:25.8475 | 4110 | 587 |
| 2. | 4. | Nature Coast | Crystal River | Mia Deronda, Leah Marville, Mackenzie Bell, Aliyah Dipalma | Braylee Spiddle, Sierra Grantham, Karilynn Willoughby, Julianna Chavavvaria | 10:27.948 | 10:26.119 | 80 | 80 |
| 3. | 1. | Citrus | Hernando | Mackenzie Scordato, Taylor Koon, Kendall Gibson, Nicole Ervin | Sadie Chomka, Jessica Maher, Jenna Stahlgren, Christyn Trappey | 38:10:38.869 | 11:06.2454 | 05684 | 4110 |
| 4. | 9. | Land O'Lakes | Bishop McLaughlin | Estefania Acosta, Maranda Hildebrand, Elise Noll, Emily Flaherty | | 10:44.82 | 38:10:38.869 | 587 | 1 |
| 5. | 5. | Fivay | Citrus | | Alexandra Meyer, Holly Niggebrugge, Lauren Nunag, Whitnee Anderson | 0:11:04.9339 | 12:25.8475 | 50494 | 1 |
| 6. | 10. | Hernando | Weeki Wachee | Madison Zuccheri, Heaven-Leigh Jackson, Jhayda Blake, Kara Wilson | Braylee Spiddle, Sierra Grantham, Karilynn Willoughby, Julianna Chavavvaria | 11:06.2454 | 0:11:04.9339 | 34 | 4110 |
| 7. | 4. | Weeki Wachee | Hernando | Natalie Evans, Giavanna Seaman, Alexis Ledbetter, Eva Newhall | Ivey Francher, Alyssa Williams, Yanializ Bonnelly, Kylie Kanaar | 11:16.2258 | 11:06.2454 | 83227 | 1 |
| 8. | 8. | Crystal River | Bishop McLaughlin | Braylee Spiddle, Sierra Grantham, Karilynn Willoughby, Julianna Chavavvaria | Estefania Acosta, Maranda Hildebrand, Elise Noll, Emily Flaherty | 11:18.849 | 10:26.119 | 1 | 4110 |
| 9. | 5. | Bishop McLaughlin | Hernando | Alexandra Meyer, Holly Niggebrugge, Lauren Nunag, Whitnee Anderson | Ivey Francher, Alyssa Williams, Yanializ Bonnelly, Kylie Kanaar | 12:25.8475 | 11:18.849 | - | 1 |
| 10. | 5. | Cypress Creek (Wesley Chapel) | Bishop McLaughlin | Sadie Chomka, Jessica Maher, Jenna Stahlgren, Christyn Trappey | Braylee Spiddle, Sierra Grantham, Karilynn Willoughby, Julianna Chavavvaria | 46:13:31.767 | 38:10:38.869 | - | 50494 |
