| 1. | 5. | Winter Park | DeLand | Emma Noel, Gabrielle Johnson , Karina Groff, Jazlin Dailey | Jaden Teow, Tava Goerner, Reema Ismail, Gabriella Macedo | 13:08.97 | 15:18.515 | 44107 | - |
| 2. | 1. | Lake Minneola | Celebration | Elena Caldwell, Jordyn Banks, Cadi Rowe, Ellie Kunze | Kayla Martinez, Adara Bautista, Bryanna King, Araceliz Rosario | 25:13:38.8222 | DNS | 12873 | - |
| 3. | 3. | Lake Howell | Lake Minneola | Isabela Zapata, Makayla Watson, Rachel St Angelo, Juliet St Angelo | Jaden Teow, Tava Goerner, Reema Ismail, Gabriella Macedo | 13:49.33 | 32:13:52.0201 | 6 | 44107 |
| 4. | | Timber Creek | DeLand | Vivian Byrnes, Alexis Gonzalez, Sierra Orie, Daria Cuellar Hernandez | Jaden Teow, Tava Goerner, Reema Ismail, Gabriella Macedo | 32:13:52.0201 | 14:14.78 | 5 | 126 |
| 5. | 3. | Edgewater | Winter Park | Gabrielle Brice, Jennifer Salinas, Sagan Simmons, Marina Brown | Isabela Zapata, Makayla Watson, Rachel St Angelo, Juliet St Angelo | 30:13:58.90 | 32:13:52.0201 | 645 | 12873 |
| 6. | 10. | Boone | Lake Minneola | Carolyn Brown, Natalie Snead, Cele Philips, Giula Ghirardi | Jaden Teow, Tava Goerner, Reema Ismail, Gabriella Macedo | 14:14.78 | 30:13:58.90 | 3 | 6 |
| 7. | 1. | Celebration | Lake Minneola | Jaden Teow, Tava Goerner, Reema Ismail, Gabriella Macedo | Shauna Mignella, Jenna Baumann, Georgia Lee, Madison Skelton | 14:18.48 | 25:13:38.8222 | 126 | 8917 |
| 8. | 9. | Lake Mary | Edgewater | Brooke Perry, Jadyn Garnett-Alvarez, Holly Gates, Emily Pounds | Jaden Teow, Tava Goerner, Reema Ismail, Gabriella Macedo | 15:12.5038 | 13:08.97 | 8917 | 645 |
| 9. | 3. | Colonial | Colonial | Kayla Martinez, Adara Bautista, Bryanna King, Araceliz Rosario | Isabela Zapata, Makayla Watson, Rachel St Angelo, Juliet St Angelo | 15:18.515 | 13:49.33 | - | 44107 |
| 10. | | DeLand | East River | Shauna Mignella, Jenna Baumann, Georgia Lee, Madison Skelton | Elena Caldwell, Jordyn Banks, Cadi Rowe, Ellie Kunze | 35:15:53.615 | 15:12.5038 | - | 645 |
| | 8. | East River | Foundation Academy | Maryha Andino, Alexandra Abud, Karina Vega, Allianie Delgado | Isabela Zapata, Makayla Watson, Rachel St Angelo, Juliet St Angelo | DNS | 13:08.97 | - | - |
| | 3. | Foundation Academy | Lake Mary | Jessica Williams, Tristen Ragsdale, Paige Ferrell, Leeley Waters | Isabela Zapata, Makayla Watson, Rachel St Angelo, Juliet St Angelo | DNS | 30:13:58.90 | - | - |
