| 1. | 10. | Auburndale | George Jenkins | Toniann Marshall, Riniya Johnson, Deniasha Jorda, Cathrina Morris | Hayley Swartz, Ainsley Aycock, Anayah Elston, Starsheena Calixte | 23:04:09.395 | DNS | 1009 | 4742 |
| 2. | 7. | Lake Wales | Lake Region | Jayla Greene, Mysha Graham, Trinity Morrison, Amber Huffman | Gwen Guillaume, Jakira Leatherwood, Keisey Tufino, Keilah Tufino | 6:04:17.211 | 6:04:17.211 | 2785 | 1 |
| 3. | 8. | George Jenkins | Lake Wales | Sydney Spaulding, Kaleigh Malick, Lauren Musselman, Alicia Ruiz | Hailey Colon, Courtnie Stanley, Dailyn Williams, Trinity Fuery | 18:04:18.561 | 23:04:09.395 | 66 | 1009 |
| 4. | 6. | Winter Haven | Ridge Community | Ashanti King, JoaQuilya Jackson, Victoria Augustin, Mariah Brown-Tullis | Ajaida Groomes, Aisjah Hunter, April James, Wislande Pierre-Louis | 4:20.0941 | 12:04:44.4584 | 059 | 4742 |
| 5. | | Kathleen | Lake Region | Vel'Lance Wells, Jo'Kierrea Kelly, Dezarae Wright | Jayla Greene, Mysha Graham, Trinity Morrison, Amber Huffman | 4:20.896 | 18:04:18.561 | 4742 | 4742 |
| 6. | 10. | Lake Gibson | Lake Region | Alana Bush, Kadaysia Dash, Czerise Villiers, Venessa Martin | Sydney Spaulding, Kaleigh Malick, Lauren Musselman, Alicia Ruiz | 13:04:35.34 | 18:04:18.561 | 39 | - |
| 7. | 3. | Frostproof | George Jenkins | Ma'Kayla Cobb, Isabel Aguilar , Arielye Baker, Laila Brown | Jayla Greene, Mysha Graham, Trinity Morrison, Amber Huffman | 4:39.43 | 18:05:34.82 | 270 | - |
| 8. | 2. | Ridge Community | Frostproof | Gwen Guillaume, Jakira Leatherwood, Keisey Tufino, Keilah Tufino | Ma'Kayla Cobb, Isabel Aguilar , Arielye Baker, Laila Brown | 12:04:44.4584 | 18:05:34.82 | 1 | 4742 |
| 9. | 9. | Bartow | Lakeland | Hailey Colon, Courtnie Stanley, Dailyn Williams, Trinity Fuery | Toniann Marshall, Riniya Johnson, Deniasha Jorda, Cathrina Morris | 4:52.8032 | 23:04:09.395 | - | - |
| 10. | | Lakeland | Winter Haven | Hayley Swartz, Ainsley Aycock, Anayah Elston, Starsheena Calixte | Hailey Colon, Courtnie Stanley, Dailyn Williams, Trinity Fuery | 18:05:34.82 | 6:04:17.211 | - | 059 |
| | | Lake Region | Kathleen | Ajaida Groomes, Aisjah Hunter, April James, Wislande Pierre-Louis | Gwen Guillaume, Jakira Leatherwood, Keisey Tufino, Keilah Tufino | DNS | DNS | - | 39 |
