| 1. | | Cocoa | John Carroll | Domanique Knowles, Shaniya Thomas, Maggie Harp, Jatana Folston | LeAysha Duncan, Amber Clark, Nya Senior, Christen McCann | 3:52.92 | DNS | 1022 | 1022 |
| 2. | | Rockledge | Viera | Tamara Buckmaster, Victoria Joyce, Kamari Dawn, Tykira Glasco | | 26:04:11.7378 | DNS | 82 | - |
| 3. | | Vero Beach | Centennial | | Domanique Knowles, Shaniya Thomas, Maggie Harp, Jatana Folston | 41:04:23.7514 | 4:36.4391 | 62694 | - |
| 4. | 9. | Fort Pierce Westwood | Fort Pierce Central | Micah Lee, Paris Grier, Tamia Robinson, Toniyah Stovall | Tamara Buckmaster, Victoria Joyce, Kamari Dawn, Tykira Glasco | 45:04:33.41 | 5:16.7565 | 55 | 62694 |
| 5. | 1. | Heritage | Fort Pierce Westwood | Abigail Baxter, Adriana Dixon, Malaysha Hardy, Courtney Simpkins | Kiana Vereen, Mackenzie Ramsey, Kammile Melandes, Malory Moroz | 31:04:34.6665 | DNS | 98402 | - |
| 6. | 9. | Centennial | Lincoln Park | Rolizbeth Acosta, Ashlyn Lecrone, Kiara Badalo, Jalissa Floristal | Elizabeth Kenney, Jenny Miller, Alexys Weideman, Isabelle Boehning | 4:36.4391 | 31:04:34.6665 | 9354 | 82 |
| 7. | 7. | Port St. Lucie | Rockledge | Sasha Walker, Jeanette Anderson, Aniah Joseph, Haylynn Tate | Abigail Baxter, Adriana Dixon, Malaysha Hardy, Courtney Simpkins | 47:04:43.0285 | DNS | 2227 | - |
| 8. | | Fort Pierce Central | Fort Pierce Central | Kiana Vereen, Mackenzie Ramsey, Kammile Melandes, Malory Moroz | Micah Lee, Paris Grier, Tamia Robinson, Toniyah Stovall | 55:04:47.78 | DNS | 018 | 2227 |
| 9. | 3. | West Shore | Fort Pierce Westwood | Laura Shelton, Alexa Weaver, Sarah Green, Kelly Ann Logan | Jaeda Stewart, Madelyn Shafer, Emily Flagg, Jasmine Johnson | 44:04:49.9151 | DNS | - | - |
| 10. | | Melbourne | Fort Pierce Westwood | Hagen Ziade, Adrianna Cato, Jolene Santos, Mikayla Morin | Sasha Walker, Jeanette Anderson, Aniah Joseph, Haylynn Tate | 0:04:58.47 | 4:36.4391 | - | 55 |
| 11. | 1. | John Carroll | Port St. Lucie | Elizabeth Kenney, Jenny Miller, Alexys Weideman, Isabelle Boehning | Syniah Johnson, Madison Webster, Cheyenne Solis, Jodie Ramos | 5:16.7565 | 4:36.4391 | - | - |
| | | Space Coast | Heritage | Syniah Johnson, Madison Webster, Cheyenne Solis, Jodie Ramos | Katie Rieke, Gretchen Lane, Claire Lockhart, Yolanda Aybar | DNS | DNS | - | - |
| | | East River | Space Coast | Beonni Smith, Natalie Soltys, Arianna Pichardo, Karina Vega | Abigail Baxter, Adriana Dixon, Malaysha Hardy, Courtney Simpkins | DNS | DNS | - | 018 |
| | | Viera | Lincoln Park | Jaeda Stewart, Madelyn Shafer, Emily Flagg, Jasmine Johnson | Sasha Walker, Jeanette Anderson, Aniah Joseph, Haylynn Tate | DNS | 3:52.92 | - | 018 |
| | | Lincoln Park | Vero Beach | LeAysha Duncan, Amber Clark, Nya Senior, Christen McCann | Syniah Johnson, Madison Webster, Cheyenne Solis, Jodie Ramos | DNS | DNS | - | - |
| | 1. | Melbourne Central Catholic | Heritage | Katie Rieke, Gretchen Lane, Claire Lockhart, Yolanda Aybar | Abigail Baxter, Adriana Dixon, Malaysha Hardy, Courtney Simpkins | DNS | 26:04:11.7378 | - | 82 |
