| 1. | 13. | Windermere | University (Orlando) | Luis Rafael Bucad, Tyler Chisholm, Noah Ellenback, Joshua Colon | Isaiah Andrews, Jalberto Sepulveda, Beloveson Philippe, Duraldo Exume | 8:09.7770 | 12:08:27.335 | 83103 | - |
| 2. | 5. | Lake Brantley | Bartow | Joab Francois, Cole Parnell , Trent Shackelford, Redwimy Noel | Omarion Sherwood, Jesse Watson, Jalen Jackson, Anthony Camacho | 8:17.89 | 8:09.7770 | 88 | - |
| 3. | 6. | Apopka | University (Orlando) | Jevon Clarke, Jackson Davis, Kyrease Desseau, Tyriq Cheatwood | Elijah Brown, Gabriel Brown, Maleik Brown, Xzabien Brown | 12:08:27.335 | 3:09:07.941 | 628 | - |
| 4. | 4. | Olympia | Olympia | Troy O'toole, Shmael Jacques, Jamal Slaven, Leron Sheppard | Seth Adams, Dain Braun, Joshua Arroyo , Nicolas Rivera | 8:29.9150 | 52:09:38.5392 | 855 | 855 |
| 5. | 4. | West Orange | Olympia | Artise Lewis, Jhaniel Navarro Ortiz, Jackson Noegel, Colton Berquist | Isaiah Andrews, Jalberto Sepulveda, Beloveson Philippe, Duraldo Exume | 30:08:38.038 | 9:03.917 | 45 | 2028 |
| 6. | 5. | Osceola (Kissimmee) | Apopka | Marvin Gatlin, Rognie Ortiz, Jacquez Jones, Elijah Gotshall | Jevon Clarke, Jackson Davis, Kyrease Desseau, Tyriq Cheatwood | 8:52.688 | 9:03.917 | 1632 | - |
| 7. | 1. | South Lake | Villages | Talon Richardson, Jose Rivas, Jose Gonzales, Alexander Croy | Artise Lewis, Jhaniel Navarro Ortiz, Jackson Noegel, Colton Berquist | 33:09:03.7277 | 3:09:07.941 | 2028 | 1632 |
| 8. | 10. | East Ridge | West Orange | Rey Diaz, Hansel Lugo Ramos, Charles Moore, Karlos Neymour | Joab Francois, Cole Parnell , Trent Shackelford, Redwimy Noel | 9:03.917 | 9:10:17.825 | 13110 | 83103 |
| 9. | 11. | University (Orlando) | Lake Brantley | Nicolas Afanador, Damarre Martin, JonAnthony Hutton, Joey Rodriguez | Kevin Butler, Anish Das, Jehu Clarke, Reginald Horton | 3:09:07.941 | 8:29.9150 | - | - |
| 10. | 15. | Lyman | Lyman | Gavin Puglisi, Alexander Vaughn, Joseph Royston, Dhruv Shah | Omarion Sherwood, Jesse Watson, Jalen Jackson, Anthony Camacho | 9:21.371 | 9:41.033 | - | - |
| 11. | 13. | Edgewater | Osceola (Kissimmee) | Kevin Butler, Anish Das, Jehu Clarke, Reginald Horton | Elijah Brown, Gabriel Brown, Maleik Brown, Xzabien Brown | 52:09:38.5392 | 52:09:38.5392 | - | - |
| 12. | 12. | Villages | West Orange | Seth Adams, Dain Braun, Joshua Arroyo , Nicolas Rivera | Isaiah Andrews, Jalberto Sepulveda, Beloveson Philippe, Duraldo Exume | 9:39.46 | 9:10:17.825 | - | 45 |
| 13. | 11. | Boone | Olympia | Cody Cunningham, Brayan Correa, Ethan Hornbeck, Karl van Gelder | Kevin Butler, Anish Das, Jehu Clarke, Reginald Horton | 9:41.033 | 8:29.9150 | - | - |
| 14. | 11. | Bartow | Olympia | Elijah Brown, Gabriel Brown, Maleik Brown, Xzabien Brown | Nicolas Afanador, Damarre Martin, JonAnthony Hutton, Joey Rodriguez | 27:09:42.0327 | 8:52.688 | - | - |
| 15. | 16. | Ocoee | Edgewater | Omarion Sherwood, Jesse Watson, Jalen Jackson, Anthony Camacho | Elijah Brown, Gabriel Brown, Maleik Brown, Xzabien Brown | 40:09:42.66 | 8:52.688 | - | - |
| 16. | 11. | Oak Ridge | Lake Brantley | Isaiah Andrews, Jalberto Sepulveda, Beloveson Philippe, Duraldo Exume | Troy O'toole, Shmael Jacques, Jamal Slaven, Leron Sheppard | 9:10:17.825 | 9:10:17.825 | - | - |
