| 1. | 8. | Carrollwood | Shorecrest Prep | Camila Garanton, Emma Architetto, Tarriyonna Gary, Sophie Haag | Breanna Hailes, Jessica Hall, Naya Hart, Addison Besedic | 9549.3325 | 955.06 | 108 | - |
| 2. | 5. | St. John Neumann | Evangelical Christian | Bianca Jean-Felix, Natalie Bongirno, Grace Kane, Leah Martin-Gonzales | Victoria Lasala, Martha Tanner, Bella Riechers, Rachel Boyd | 7550.3232 | 7455.69 | 78 | 78 |
| 3. | | Master's Academy (Oviedo) | Clearwater Central Catholic | Victoria Lasala, Martha Tanner, Bella Riechers, Rachel Boyd | Victoria Lasala, Martha Tanner, Bella Riechers, Rachel Boyd | 6851.9949 | 252.362 | 3162 | 3162 |
| 4. | 4. | Victory Christian (LL) | Victory Christian (LL) | Breanna Alexander, Lilian Acevedo, Issy Pita, Brielle Liverman | Madison Hickey, Paris Avery, Reem Saidy, Kendall Liermann | 252.362 | 6851.9949 | 75 | - |
| 5. | 6. | Holy Names | Victory Christian (LL) | Britton Byrd, Rebekah Eicholtz, Catalina Gonzalez, Isabella Yepez | Kayleigh Vogt, Autumn Sun, Nikole Cosentino, Brooke Cosentino | 652.489 | 955.06 | 546 | - |
| 6. | 6. | Clearwater Central Catholic | Master's Academy (Oviedo) | Madison Hickey, Paris Avery, Reem Saidy, Kendall Liermann | Marshanna Brown, Tyanna Terrel, Kaylynn Williamson, Megan McNichols | 54.4438 | 955.06 | 373 | - |
| 7. | | St. Stephens Episcopal | Victory Christian (LL) | Kayleigh Vogt, Autumn Sun, Nikole Cosentino, Brooke Cosentino | Marshanna Brown, Tyanna Terrel, Kaylynn Williamson, Megan McNichols | 955.06 | 1:09.56 | 26281 | 373 |
| 8. | 4. | Evangelical Christian | Calvary Christian (Clr) | Alexa Bereczki, Claire Valentine, Manon Masso, Kristen Julmeus | Britton Byrd, Rebekah Eicholtz, Catalina Gonzalez, Isabella Yepez | 7455.69 | 7455.69 | 13 | 373 |
| 9. | 2. | Pepin Academy | St. Stephens Episcopal | Marshanna Brown, Tyanna Terrel, Kaylynn Williamson, Megan McNichols | Bianca Jean-Felix, Natalie Bongirno, Grace Kane, Leah Martin-Gonzales | 1:09.56 | 7550.3232 | - | 108 |
| | 9. | Cambridge Christian | Shorecrest Prep | Anna Woliver, Brooke Williams, Brooke Delfavero, Alayyah Washington | Victoria Lasala, Martha Tanner, Bella Riechers, Rachel Boyd | DQ | 7455.69 | - | 3162 |
| | 9. | Shorecrest Prep | Evangelical Christian | Renice Dacres, Mikera Patton, Alanis Job, Devyn Kropf | Bianca Jean-Felix, Natalie Bongirno, Grace Kane, Leah Martin-Gonzales | DNS | DQ | - | 3162 |
| | 8. | Calvary Christian (Clr) | Master's Academy (Oviedo) | Breanna Hailes, Jessica Hall, Naya Hart, Addison Besedic | Breanna Hailes, Jessica Hall, Naya Hart, Addison Besedic | DNS | DNS | - | 108 |
