| 1. | 8. | Evangelical Christian | First Baptist (Naples) | Sydney Land, Elizabeth Wetmore, Sierra Oliveira, Moriah Oliveira | Rebekah Eicholtz, Megan Hughes, Catalina Gonzalez, Britton Byrd | 4:06.4175 | 14:04:53.1478 | 110 | 07851 |
| 2. | | Berkeley Prep | Master's Academy (Oviedo) | Sydney Mercurio, Catelin Lipham, Ally Aves, Carsyn Martz | Ari Cintron, Avery Erikson, Sloane Janopoulos, Malaya Melancon | 4:10.36 | DNS | 07851 | - |
| 3. | 9. | St. John Neumann | Carrollwood | Natalie Bongirno, Bianca Jean-Felix, Ashley De Armas, Grace Kane | Ari Cintron, Avery Erikson, Sloane Janopoulos, Malaya Melancon | 4:17.9030 | 4:39.45 | 604 | 34 |
| 4. | 4. | Master's Academy (Oviedo) | Berkeley Prep | Martha Tanner, Victoria Lasala, Bella Riechers, Rachel Boyd | Ari Cintron, Avery Erikson, Sloane Janopoulos, Malaya Melancon | 4:19.16 | 4:19.16 | 524 | 07851 |
| 5. | 5. | Holy Names | St. Stephens Episcopal | Rebekah Eicholtz, Megan Hughes, Catalina Gonzalez, Britton Byrd | Kailyn Gilbert, Camille Samuel, Maggie Matulis, Summer Haura | 4:19.9109 | 4:10.36 | 0244 | - |
| 6. | | Clearwater Central Catholic | Victory Christian (LL) | Keely Karnes, Kasey Singer, Molly Simmons, Emily Rich | Natalie Bongirno, Bianca Jean-Felix, Ashley De Armas, Grace Kane | 27:04:20.4344 | DNS | 34 | - |
| 7. | | Tampa Prep | Calvary Christian (Clr) | Kailyn Gilbert, Camille Samuel, Maggie Matulis, Summer Haura | Addison Besedic, Grace Galloway, Naya Hart, Breanna Hailes | 36:04:27.139 | 4:17.9030 | 6020 | 110 |
| 8. | 6. | St. Stephens Episcopal | Calvary Christian (Clr) | Kayleigh Vogt, Demi Harms, Jaclyn Schlossberg, Taylor Vogt | Natalie Bongirno, Bianca Jean-Felix, Ashley De Armas, Grace Kane | 4:39.45 | 27:04:20.4344 | 7613 | 7613 |
| 9. | 9. | Victory Christian (LL) | Clearwater Central Catholic | Kirsten Forman, Issy Pita, Rayna Lemmond, Kaylie Whittenton | Ari Cintron, Avery Erikson, Sloane Janopoulos, Malaya Melancon | 14:04:53.1478 | 36:04:27.139 | - | 110 |
| 10. | 2. | Carrollwood | Evangelical Christian | Anjali Panchal, Matheson Woeste, Brooke Staten, Hannah McCartney | Rebekah Eicholtz, Megan Hughes, Catalina Gonzalez, Britton Byrd | 38:05:34.7722 | DNS | - | - |
| | 9. | Calvary Christian (Clr) | Clearwater Central Catholic | Addison Besedic, Grace Galloway, Naya Hart, Breanna Hailes | Kailyn Gilbert, Camille Samuel, Maggie Matulis, Summer Haura | DNS | 4:06.4175 | - | 110 |
| | 5. | First Baptist (Naples) | Tampa Prep | Ari Cintron, Avery Erikson, Sloane Janopoulos, Malaya Melancon | Keely Karnes, Kasey Singer, Molly Simmons, Emily Rich | DNS | 36:04:27.139 | - | - |
