| 1. | 2. | Cocoa | Apopka | Domanique Knowles, Shaniya Thomas, Daishon Spann, Jatana Folston | Brooke Reif, Nicolina Otero, Mckenzie Connor, Jinia Johnson | 13:03:49.114 | 4:02.189 | 001045 | - |
| 2. | 18. | Apopka | Cocoa | Hannah Jones, Astoria Beckett, Sydney Fowler, Samantha Andreetti | Hannah Jones, Astoria Beckett, Sydney Fowler, Samantha Andreetti | 4:02.189 | 2:04:19.0536 | 48 | 34224 |
| 3. | | Evans | Leesburg | Jahnile Registre, Janiyah Hall, Amenda St-Luis, Princess Vigilance | Maryam Khalil, Afiya Thompson, Dahlia Williams, Mya Hill | 11:04:03.66 | DNS | 63 | 51 |
| 4. | 7. | Osceola (Kissimmee) | Auburndale | Amaya Phillips, Jetzymar Rivera, Dezha Walker, Alliann Johnson | Jahnile Registre, Janiyah Hall, Amenda St-Luis, Princess Vigilance | 4:05.055 | 2:04:19.0536 | 6594 | - |
| 5. | 11. | Olympia | Harmony | Jade Kelly, Jade Blake, Alyia Green, Lauren Guthrie | Amaya Phillips, Jetzymar Rivera, Dezha Walker, Alliann Johnson | 55:04:07.07 | DNS | 43 | 53 |
| 6. | 15. | Rockledge | First Academy (Orlando) | Tamara Buckmaster, Victoria Joyce, Kamari Dawn, Tykira Glasco | Jahnile Registre, Janiyah Hall, Amenda St-Luis, Princess Vigilance | 4:08.4760 | 48:04:26.153 | 53 | - |
| 7. | 18. | Auburndale | First Academy (Orlando) | Riniya Johnson, Cathrina Morris, Toniann Marshall, Saniyah Tillman | Domanique Knowles, Shaniya Thomas, Daishon Spann, Jatana Folston | 28:04:14.386 | DNS | 34224 | 51 |
| 8. | 4. | Lake Brantley | Mount Dora Christian | Jordan Stevens, Anya Spragg, Cadeeja Pennant, Ashley Klingenberg | Julie Hicks, Alanie Rivera, Vanielis Ramos, Regina Younassoghlou | 4:16.397 | 4:19.607 | 51 | 63 |
| 9. | 11. | Ocoee | Lakewood | Ariana Rivers, Zy'Kerriah Croker, Eriona Sherwood, Miracle Johnson | Domanique Knowles, Shaniya Thomas, Daishon Spann, Jatana Folston | 29:04:16.88 | 11:04:03.66 | - | 34224 |
| 10. | 13. | Kathleen | Osceola (Kissimmee) | Vel'Lance Wells, Jo'Kierra Kelly, Ashlynn Hogg, Dezarae Wright | Jordan Stevens, Anya Spragg, Cadeeja Pennant, Ashley Klingenberg | 22:04:17.9199 | 48:04:26.153 | - | 48 |
| 11. | 2. | Wiregrass Ranch | Lake Brantley | Maryam Khalil, Afiya Thompson, Dahlia Williams, Mya Hill | Naytasha Perez, Alissa Gomez, Journey Cadle, Rebecca Dawson | 2:04:19.0536 | 5:15.623 | - | - |
| 12. | 17. | Winter Haven | Olympia | Ashanti King, Shardajah Collins, Victoria Augustin, Ralia Wester | Tatiana Butler, Ashley Hernandez, Brianna Rodriguez, Daniella Frederic | 4:19.607 | 29:04:16.88 | - | 51 |
| 13. | 2. | Harmony | Leesburg | Julie Hicks, Alanie Rivera, Vanielis Ramos, Regina Younassoghlou | Ariana Rivers, Zy'Kerriah Croker, Eriona Sherwood, Miracle Johnson | 48:04:26.153 | 5:15.623 | - | 001045 |
| 14. | 5. | Lake Minneola | Wiregrass Ranch | Jordyn Banks, Mi'Ajhai Cleaves, Brianna Julmiste, Jahliyah Spells | Julie Hicks, Alanie Rivera, Vanielis Ramos, Regina Younassoghlou | 4:27.304 | 4:19.607 | - | 34224 |
| 15. | 8. | Lake Wales | Cocoa | Jayla Greene, Dashane Medina, Trinity Morrison, Mysha Graham | Brazyl Blake, Danielle Gill, Majani Perryman, Adriel Strickland | 36:04:28.3309 | 28:04:14.386 | - | 48 |
| 16. | 12. | Lakewood | Lake Wales | Shadine Henry, Anteonia Talley, Reilly Webb, Nilah Coleman | Amaya Phillips, Jetzymar Rivera, Dezha Walker, Alliann Johnson | 4:36.82 | 5:15.623 | - | 53 |
| 17. | | Wharton | Cocoa | Brooke Reif, Nicolina Otero, Mckenzie Connor, Jinia Johnson | Shadine Henry, Anteonia Talley, Reilly Webb, Nilah Coleman | 4:39.6039 | 13:03:49.114 | - | - |
| 18. | 7. | Mount Dora Christian | Lake Minneola | Bregan Shore, Courtnei Wilson, Isabella Kirst, , | Naytasha Perez, Alissa Gomez, Journey Cadle, Rebecca Dawson | 5:15.623 | DNS | - | - |
| | 14. | First Academy (Orlando) | Ocoee | Courtney Afram-Gyening, Isabel Crist, Sharya Davis, Ella Simpson | Courtney Afram-Gyening, Isabel Crist, Sharya Davis, Ella Simpson | DNS | 5:15.623 | - | 6594 |
| | | New Dimensions | Alonso | Tatiana Butler, Ashley Hernandez, Brianna Rodriguez, Daniella Frederic | Domanique Knowles, Shaniya Thomas, Daishon Spann, Jatana Folston | DNS | 4:39.6039 | - | - |
| | | Paxon | Rockledge | Brazyl Blake, Danielle Gill, Majani Perryman, Adriel Strickland | Jahnile Registre, Janiyah Hall, Amenda St-Luis, Princess Vigilance | DNS | 2:04:19.0536 | - | - |
| | 6. | Leesburg | New Dimensions | Shiane Dabney, Kareena Gladis, Mackenzie Harrington, Christania Reed | Tatiana Butler, Ashley Hernandez, Brianna Rodriguez, Daniella Frederic | DNS | 4:36.82 | - | - |
| | | Alonso | Winter Haven | Janessa Thomas, Alyssa Bartolo, Isansa Bonga, Jarlenys Cordoba | Bregan Shore, Courtnei Wilson, Isabella Kirst, , | DNS | DNS | - | 34224 |
| | 9. | Tohopekaliga (Kissimmeee) | New Dimensions | Naytasha Perez, Alissa Gomez, Journey Cadle, Rebecca Dawson | Bregan Shore, Courtnei Wilson, Isabella Kirst, , | DNS | 4:05.055 | - | - |
