| 1. | | Master's Academy (Oviedo) | Windermere Prep | Martha Tanner, Victoria Lasala, Bella Riechers, Rachel Boyd | Christania Reed, Sh'Landria Roberts, Mackenzie Harrington, Shiane Dabney | 4:25.61 | DNS | 11038 | 15191 |
| 2. | 8. | Freedom (Orlando) | Leesburg | | Olivia Cullison, Ainsley Compher, Jeanne Davis, Adrice Galloway | 37:04:27.116 | 37:04:27.116 | 80 | - |
| 3. | | Oviedo | Citrus | Olivia Cullison, Ainsley Compher, Jeanne Davis, Adrice Galloway | | 51:04:33.879 | 25:04:36.4960 | 95639 | 1934 |
| 4. | 6. | Montverde Academy | Lake Weir | Lily Henne, Brianna Hiers, Zarrah Barbaza , Fernanda Uruchurta | | 25:04:36.4960 | 4:40.80 | 539 | - |
| 5. | 4. | Citrus | Citrus | Taylor Koon, Kendall Gibson, Sophia Woggon, Nicole Ervin | | 10:04:38.99 | 40:04:54.492 | 7448 | 11038 |
| 6. | 1. | Villages | Windermere Prep | Sydney Richason - Bugg, Rebecca Clark , Brookette Bryant, Ella Kate Daley | Christania Reed, Sh'Landria Roberts, Mackenzie Harrington, Shiane Dabney | 4:40.80 | DNS | 1934 | 15191 |
| 7. | 3. | Windermere Prep | Ridge Community | Dania Fernandez, Stephanie Sun, Ann Casey, Mirabella Miranda | Dania Fernandez, Stephanie Sun, Ann Casey, Mirabella Miranda | 31:04:43.2476 | DNS | 402 | - |
| 8. | 2. | Ridge Community | Oviedo | Sade' Ivey, Gabrielle Haines, Gianna Ennis, Jakira Leatherwood | Dania Fernandez, Stephanie Sun, Ann Casey, Mirabella Miranda | 4:46.9579 | 51:04:33.879 | 15191 | - |
| 9. | 3. | St. Francis | Ridge Community | Elizabeth Doan, Jovana Bautista, Elizabeth Muir, Milly Aidan | Dania Fernandez, Stephanie Sun, Ann Casey, Mirabella Miranda | 40:04:54.492 | 4:25.61 | - | - |
| | 3. | Leesburg | Lake Weir | Christania Reed, Sh'Landria Roberts, Mackenzie Harrington, Shiane Dabney | Courtnei Wilson, Bregan Shore, Arianna Cawthon, Kailey Daniel | DNS | 25:04:36.4960 | - | 95639 |
| | | Mount Dora Christian | Lake Weir | Courtnei Wilson, Bregan Shore, Arianna Cawthon, Kailey Daniel | Christania Reed, Sh'Landria Roberts, Mackenzie Harrington, Shiane Dabney | DNS | 10:04:38.99 | - | 15191 |
| | 1. | Lake Weir | Villages | Sinai Lee, Olivia Wade, Jomyra Green, Toriana McCoy | Sade' Ivey, Gabrielle Haines, Gianna Ennis, Jakira Leatherwood | DNS | 4:46.9579 | - | - |
