| 1. | 12. | Lake Brantley | St. Cloud | Chloe Hyde, Emma Powell, Jordan Stevens, Ashley Klingenberg | Tori Stone, Lindsay Ise, Mackenzie Capella, Josephine Garba | 30:09:49.64 | 11:18.2813 | 41096 | 43 |
| 2. | 1. | George Jenkins | Auburndale | Alicia Ruiz, Viktorya Manno, Anna Sentner, Kaleigh Malick | Tori Stone, Lindsay Ise, Mackenzie Capella, Josephine Garba | 10:12.90 | 11:18.2813 | 185 | 026 |
| 3. | 5. | Harmony | Ridge Community | Brooke Mullins, Claire Darrin, Ariana Ramos Mercado, Allison Hicks | Rebekah Haddad, Emma McLaughlin, Rachel Knazavich, Taya Cook | 10:54.324 | 12:08.01 | 026 | 43 |
| 4. | 1. | Rockledge | Rockledge | Victoria Joyce, Abigail Lake, Kaitlin Braswell, Shanai Banks | Sydney Slagel, Bella Blind, Jateria Parker, Nyashia Williams-Matthews | 49:10:55.4277 | 12:01.34 | 3755 | 525 |
| 5. | 6. | Lakewood Ranch | Riverview | Aurora Melzer, Jessica Thompson, Elise Dunn, Riley Bradshaw | Jasmine Valle, Tabitha Suggs, Sarah Anderson, Kya Chen | 6:11:00.246 | 12:08.01 | 43 | - |
| 6. | 2. | St. Cloud | Winter Haven | Tiana Rivera, Gabriela Lugopardo, Hazel Rodriguez, Brenna Bush | Jasmine Valle, Tabitha Suggs, Sarah Anderson, Kya Chen | 11:08.537 | 10:54.324 | 234 | 026 |
| 7. | | Auburndale | Alonso | Kathy Macedo Pineda, Kya Cadieu, Maria Manosalva, Riniya Johnson | Victoria Joyce, Abigail Lake, Kaitlin Braswell, Shanai Banks | 11:18.2813 | 11:08.537 | 525 | 026 |
| 8. | 5. | Alonso | Alonso | Jasmine Valle, Tabitha Suggs, Sarah Anderson, Kya Chen | Tiana Rivera, Gabriela Lugopardo, Hazel Rodriguez, Brenna Bush | 37:11:24.3443 | 10:54.324 | 15 | 026 |
| 9. | 3. | Riverview | Sarasota | Mansi Lorenzen , McKenna Hartigan, Emily Mason , Natalie Rohm | Lidia Leon, Cecilia Martinez-Castillo, Danielle Delgado, Juliette Crispin | 11:31.9202 | 9:11:39.82 | - | 185 |
| 10. | 2. | Plant City | Riverview | Lidia Leon, Cecilia Martinez-Castillo, Danielle Delgado, Juliette Crispin | Brooke Mullins, Claire Darrin, Ariana Ramos Mercado, Allison Hicks | 11:32.9935 | 18:12:40.613 | - | - |
| 11. | 2. | Tampa Bay HEAT | Sarasota | Rebekah Haddad, Emma McLaughlin, Rachel Knazavich, Taya Cook | Rachel Brutus, Gladys Cortez, Antigone Pesack, Karina Sanchez | 9:11:39.82 | 49:10:55.4277 | - | - |
| 12. | 15. | Tampa Prep | Alonso | Tori Stone, Lindsay Ise, Mackenzie Capella, Josephine Garba | Lidia Leon, Cecilia Martinez-Castillo, Danielle Delgado, Juliette Crispin | 14:11:43.866 | 11:31.9202 | - | 3755 |
| 13. | 4. | Ridge Community | Tampa Bay HEAT | Wileishka Rodriguez Pabon, Melanie Berrios Morales, Gabrielle Haines | Alicia Ruiz, Viktorya Manno, Anna Sentner, Kaleigh Malick | 12:01.34 | 10:12.90 | - | - |
| 14. | 2. | Lake Region | Plant City | Rachel Brutus, Gladys Cortez, Antigone Pesack, Karina Sanchez | Sydney Slagel, Bella Blind, Jateria Parker, Nyashia Williams-Matthews | 12:08.01 | 6:11:00.246 | - | 3755 |
| 15. | 8. | Winter Haven | Tampa Bay HEAT | Sydney Slagel, Bella Blind, Jateria Parker, Nyashia Williams-Matthews | Jasmine Valle, Tabitha Suggs, Sarah Anderson, Kya Chen | 18:12:40.613 | 12:08.01 | - | 234 |
| | 8. | Sarasota | Harmony | Lauren Ippolito, Camryn Wigglesworth, Kasumi Wade, Marissa Kempf | Aurora Melzer, Jessica Thompson, Elise Dunn, Riley Bradshaw | DNS | 11:08.537 | - | 525 |
