| | | Cypress Creek | Master's Academy (Oviedo) | Carine Gourdet, Mariah Thomas, Jeleicia Morgan, Tiffany Wangui | Karimar Pretto, Antonia MacCormack, Alesha Montes, Alema Belle | DQ | DNS | - | - |
| | | Mount Dora Christian | Mount Dora Christian | Brianna Hall, Isabella Kirst, Bregan Shore, Courtnei Wilson | Gabriella Figureroa, Xaniah Williams, Lanecia Wallace, Taylor Veatch | DNS | 4:40.533 | - | 8330 |
| | | Oak Ridge | Lake Mary | Naika Metellus, Falonne Michel , Fisnerline Dorcely, Adeleis Enarnacion | Naika Metellus, Falonne Michel , Fisnerline Dorcely, Adeleis Enarnacion | DNS | 7:04:16.02 | - | 467 |
| | | Foundation Academy | Montverde Academy | Faith Ford, Tristen Ragsdale, Leeley Waters, Jessica Williams | Gabrielle Brice, Marina Brown, Sagan Simmons, Jennifer Salinas | DNS | 4:35.71 | - | - |
| | | South Lake | Lake Mary | Alba Rivas, Casye Johnson, Journey Williams, Citlaly Fernandez | Brianna Hall, Isabella Kirst, Bregan Shore, Courtnei Wilson | DNS | 4:37.771 | - | - |
| | | Tohopekaliga (Kissimmeee) | University (OC) | Karimar Pretto, Antonia MacCormack, Alesha Montes, Alema Belle | Alba Rivas, Casye Johnson, Journey Williams, Citlaly Fernandez | DNS | 7:04:16.02 | - | - |
| | | Hardee | Edgewater | Kaitlynn Brandeberry, Acheline Delhomme, Jessica Pascual, Daniella Marrero | Bryanna King, Zaria Bailey, Arianna Bailey, Leyshka Arroyo | DNS | 4:30.111 | - | 424 |
| | | Liberty | Palatka | Kanitria Antenor, Emilie Edouard, Nyidjah Ives, Alandi Jacobs | Naika Metellus, Falonne Michel , Fisnerline Dorcely, Adeleis Enarnacion | 4:12.1766 | DNS | 310 | 424 |
| | | Spruce Creek | University (OC) | Kaylee Van Rij, Paige Cooksey, Naiya Pettway , Elise Butler | Naika Metellus, Falonne Michel , Fisnerline Dorcely, Adeleis Enarnacion | 7:04:16.02 | 4:37.771 | 886 | - |
| | | Dr. Phillips | Liberty | Jarvia Carr, Domanique Douglas, Lourdes Saffird, Rayanna Mathis | Evie Erikson, Cymiah Bradley, Christina Labrato, Isabelle Zadzban | 1:04:16.9850 | 23:04:25.8198 | 467 | 424 |
| | | Lake Nona | Spruce Creek | Justyn Smith, Stephanie Towle, Kisha Forester, Sydni Brown | Sydney Richason - Bugg, Rebecca Clark , Brookette Bryant, Ella Kate Daley | 23:04:25.8198 | 4:56.41 | 58 | - |
| | | Lake Mary | Lake Nona | Evie Erikson, Cymiah Bradley, Christina Labrato, Isabelle Zadzban | Evie Erikson, Cymiah Bradley, Christina Labrato, Isabelle Zadzban | 4:30.111 | 4:35.05 | 7142 | 58 |
| | | Colonial | Tohopekaliga (Kissimmeee) | Bryanna King, Zaria Bailey, Arianna Bailey, Leyshka Arroyo | Gabrielle Brice, Marina Brown, Sagan Simmons, Jennifer Salinas | 4:31.400 | DNS | 8330 | - |
| | | Palatka | Palatka | Diovinne Fells, Iyanna Woods, Cartashous Valentines, Tykidra Leonard | Hailey Colon, Trinity Fuery, TaÃÂÃÂMetria Graydon, Courtnie Stanley | 4:33.75 | 4:12.1766 | 424 | - |
| | | North Marion | Montverde Academy | Kinitty Blackwell, Brie Burrell, Allyson Gilmore, Ke'Aria Henderson | Hailey Colon, Trinity Fuery, TaÃÂÃÂMetria Graydon, Courtnie Stanley | 6:04:34.80 | 4:58.259 | 7013 | - |
| | | Forest | Bartow | Robyn Guest, Keala McKinley, Nichole Harris, Lauren Sacauskas | Briahna Martin, Abigail Thompson, Isabella Hernandez, Kiley Stahlman | 4:35.05 | 6:04:34.80 | - | 8330 |
| | | Buchholz | Master's Academy (Oviedo) | Gabriella Figureroa, Xaniah Williams, Lanecia Wallace, Taylor Veatch | Adeena Ademu John, Sheintah Artison, Amelia Vasquez, Ilona Zimmer | 4:35.71 | 4:43.269 | - | - |
| | | Master's Academy (Oviedo) | University (OC) | Martha Tanner, Victoria Lasala, Sarah Davis, Bella Riechers | Martha Tanner, Victoria Lasala, Sarah Davis, Bella Riechers | 4:35.866 | 4:35.71 | - | - |
| | | Windermere | Windermere | Adeena Ademu John, Sheintah Artison, Amelia Vasquez, Ilona Zimmer | Briahna Martin, Abigail Thompson, Isabella Hernandez, Kiley Stahlman | 4:36.63 | 4:37.771 | - | - |
| | | Edgewater | Villages | Gabrielle Brice, Marina Brown, Sagan Simmons, Jennifer Salinas | Robyn Guest, Keala McKinley, Nichole Harris, Lauren Sacauskas | 4:37.771 | DNS | - | - |
| | | Montverde Academy | Mount Dora Christian | Erin Dumas, Madison McMullen , Katherine Chen, Karah White | Jarvia Carr, Domanique Douglas, Lourdes Saffird, Rayanna Mathis | 4:40.533 | 1:04:16.9850 | - | - |
| | | Villages | Hardee | Sydney Richason - Bugg, Rebecca Clark , Brookette Bryant, Ella Kate Daley | Adeena Ademu John, Sheintah Artison, Amelia Vasquez, Ilona Zimmer | 4:43.269 | DNS | - | - |
| | | University (OC) | Palatka | Briahna Martin, Abigail Thompson, Isabella Hernandez, Kiley Stahlman | Bryanna King, Zaria Bailey, Arianna Bailey, Leyshka Arroyo | 4:56.41 | 4:36.63 | - | 58 |
| | | Bartow | Foundation Academy | Hailey Colon, Trinity Fuery, TaÃÂÃÂMetria Graydon, Courtnie Stanley | Evie Erikson, Cymiah Bradley, Christina Labrato, Isabelle Zadzban | 4:58.259 | 4:31.400 | - | - |
