| 15. | 1. | 9. | Land O'Lakes | Mainland | Belleview | Greysun Ledy, Paige Ramsey, Lauren Sacauskas, Margaret Crowe | Jordan Elder, Leeann Kent, Grace Hawryluk, Dnajah Smith | Yanializ Bonnelly, Maya Rhymer, Ivey Francher, Kylie Kanaar | 44:11:06.606 | 32:10:00.903 | 10:19.8669 | 1247 | 10 | 55 |
| 8. | 2. | 1. | Forest | Forest | Lake Region | Lashonta Joe, Rachel Patrick, Isa Carrillo, Airon Rhoden | Greysun Ledy, Paige Ramsey, Lauren Sacauskas, Margaret Crowe | Mykenzee Marshall, Julia Charske, Jillian Kay, Riley Martin | 10:09.1179 | 22:10:06.839 | 11:13.4034 | - | 9780 | 22 |
| 11. | 3. | 11. | Matanzas | Harmony | River Ridge | Grace Smith, Mia Striegel, Kimberly Collins, Kristan Wallace | Brooke Mullins, Claire Darrin, Vanessa Bolen, Allison Hicks | Safi Chalfin-Smith, Emma Beachy, Scotlyn Silvey, Natalie Allen | 11:10.250 | 10:09.1179 | 10:40.7028 | - | 6 | 17 |
| 8. | 4. | 16. | Springstead | Belleview | Auburndale | Grace Smith, Mia Striegel, Kimberly Collins, Kristan Wallace | Katerina Dobkowski, Teagan Youngquist, Kaitlyn Beckwith, Kaleah Florence | Emma Devlin, Felicia Paul, Shannan Korovich, Jessica Jadoda | 32:10:00.903 | 10:16.334 | 10:36.197 | - | 55 | 1247 |
| 7. | 5. | 16. | Seabreeze | Springstead | Gainesville | Kelly Steffens, Morgan Sharman, Allison Tuggle, Rain Marti | Yanializ Bonnelly, Maya Rhymer, Ivey Francher, Kylie Kanaar | Shannon Gordy, Cathryne Newport, Ashley Spires, Mariah Morales | 10:19.8669 | 10:18.5374 | 10:18.5374 | - | 1247 | - |
| 13. | 6. | 16. | Belleview | Matanzas | Vanguard (Ocala) | Emma Schlageter, Summer Ward, Julia Hinkle, Kayla Horvath | Kelly Steffens, Morgan Sharman, Allison Tuggle, Rain Marti | Emma Schlageter, Summer Ward, Julia Hinkle, Kayla Horvath | 11:13.4034 | 10:19.8669 | 22:10:06.839 | 1247 | 3735 | 55 |
| 5. | 7. | 5. | Springstead | Vanguard (Ocala) | Harmony | Mykenzee Marshall, Julia Charske, Jillian Kay, Riley Martin | Mykenzee Marshall, Julia Charske, Jillian Kay, Riley Martin | Kelly Steffens, Morgan Sharman, Allison Tuggle, Rain Marti | 10:18.5374 | 7:10:20.781 | 10:36.197 | 9780 | 22 | 6 |
| 12. | 8. | 5. | Land O'Lakes | Lake Region | Mainland | Yvena Louis, Wislande Pierre-Louis, Jashantis Moore, April James | Yvena Louis, Wislande Pierre-Louis, Jashantis Moore, April James | Greysun Ledy, Paige Ramsey, Lauren Sacauskas, Margaret Crowe | 3:11:30.434 | 10:36.197 | 32:10:00.903 | 9780 | 17 | 6 |
| 16. | 9. | 2. | Bartow | Sunlake | Auburndale | Mikayla Baker, Samantha Zaw, Elise Noll, Emily Flaherty | Shannon Gordy, Cathryne Newport, Ashley Spires, Mariah Morales | Jordan Elder, Leeann Kent, Grace Hawryluk, Dnajah Smith | 10:19.8669 | 10:40.7028 | 10:16.334 | 17 | - | 17 |
| 8. | 10. | 12. | Forest | Auburndale | New Smyrna Beach | Jordan Elder, Leeann Kent, Grace Hawryluk, Dnajah Smith | Kathy Macedo Pineda, Hannia Guerrero Hernan, Riniya Johnson, Victoria Morris | Kathy Macedo Pineda, Hannia Guerrero Hernan, Riniya Johnson, Victoria Morris | 10:09.1179 | 45:10:56.666 | 3:11:30.434 | 55 | - | 17 |
| 9. | 11. | 8. | Seabreeze | Land O'Lakes | Mainland | Safi Chalfin-Smith, Emma Beachy, Scotlyn Silvey, Natalie Allen | Mikayla Baker, Samantha Zaw, Elise Noll, Emily Flaherty | Emma Schlageter, Summer Ward, Julia Hinkle, Kayla Horvath | 10:18.5374 | 11:04.4921 | 11:10.250 | 6 | - | - |
| 16. | 12. | 4. | Belleview | Gainesville | Springstead | Emma Schlageter, Summer Ward, Julia Hinkle, Kayla Horvath | Safi Chalfin-Smith, Emma Beachy, Scotlyn Silvey, Natalie Allen | Emma Devlin, Felicia Paul, Shannan Korovich, Jessica Jadoda | 11:04.4921 | 44:11:06.606 | 11:10.250 | 22 | - | 9780 |
| 8. | 13. | 10. | Vanguard (Ocala) | River Ridge | Sunlake | Yvena Louis, Wislande Pierre-Louis, Jashantis Moore, April James | Emma Devlin, Felicia Paul, Shannan Korovich, Jessica Jadoda | Yvena Louis, Wislande Pierre-Louis, Jashantis Moore, April James | 3:11:30.434 | 11:10.250 | 11:10.250 | 22 | - | - |
| 9. | 14. | 11. | Land O'Lakes | New Smyrna Beach | Seabreeze | Kathy Macedo Pineda, Hannia Guerrero Hernan, Riniya Johnson, Victoria Morris | Emma Schlageter, Summer Ward, Julia Hinkle, Kayla Horvath | Yvena Louis, Wislande Pierre-Louis, Jashantis Moore, April James | 10:19.8669 | 11:13.4034 | 3:11:30.434 | 22 | - | 17 |
| 10. | 15. | 7. | New Smyrna Beach | Seabreeze | Gainesville | Mikayla Baker, Samantha Zaw, Elise Noll, Emily Flaherty | Grace Smith, Mia Striegel, Kimberly Collins, Kristan Wallace | Greysun Ledy, Paige Ramsey, Lauren Sacauskas, Margaret Crowe | 10:18.5374 | 3:11:30.434 | 10:40.7028 | 22 | - | - |
| 3. | 16. | 5. | Mainland | Bartow | Forest | Safi Chalfin-Smith, Emma Beachy, Scotlyn Silvey, Natalie Allen | Lashonta Joe, Rachel Patrick, Isa Carrillo, Airon Rhoden | Yanializ Bonnelly, Maya Rhymer, Ivey Francher, Kylie Kanaar | 10:19.8669 | 11:58.6049 | 44:11:06.606 | 55 | - | - |
