| 1. | 4. | Winter Springs | Lake Brantley | Mackenzie Czurak, Camille Sims, Carina Clark, Elayna Bahr | Ana Figueroa, Derartu Berrios, Keila Gorritz, Daimone McCalla-Clark | 4:03.8642 | 4:12.089 | 9109 | 4638 |
| 2. | | Spruce Creek | Lake Mary | Kaylee Van Rij, Paige Cooksey, Naiya Pettway, Julianna Walker | Cheyenne Dunn, Jessica Apakama, Jordan Singletary, Vanessa Torres | 4:12.089 | 4:03.8642 | 3827 | - |
| 3. | 3. | Lake Brantley | DeLand | Rachel Ramirez, Ariel Manigault, Roselane Ramirez, Jordan Stevens | Mackenzie Czurak, Camille Sims, Carina Clark, Elayna Bahr | 59:04:16.38 | 59:04:16.38 | 6 | 3827 |
| 4. | 6. | Seminole (Sanford) | University (OC) | Ana Figueroa, Derartu Berrios, Keila Gorritz, Daimone McCalla-Clark | Cheyenne Dunn, Jessica Apakama, Jordan Singletary, Vanessa Torres | 28:04:17.820 | 4:12.089 | 555 | - |
| 5. | 2. | DeLand | Spruce Creek | Cheyenne Dunn, Jessica Apakama, Jordan Singletary, Vanessa Torres | Ana Figueroa, Derartu Berrios, Keila Gorritz, Daimone McCalla-Clark | 4:25.93 | DQ | 34402 | 3827 |
| 6. | 4. | University (OC) | Winter Springs | Caitlyn Toro, Madison Leake, Briahna Martin, Abigail Thompson | Rachel Ramirez, Ariel Manigault, Roselane Ramirez, Jordan Stevens | 4:47.34 | 4:03.8642 | 4638 | 34402 |
| | 6. | Lake Mary | Spruce Creek | Cheyenne St. Lewis, Kennedie Reese, Shakiena Miles, Christina Labrato | Caitlyn Toro, Madison Leake, Briahna Martin, Abigail Thompson | DQ | 4:03.8642 | - | 555 |
