| 1
| Wright, Cydney | SR | Mainland | 23.46 | FHSAA 3A Region 1 | May 1, 2021 | 2.0 |
| 2
| Jones, Kahdia | SR | Atlantic (PO) | 25.66 | FHSAA 2A Region 2 | May 1, 2021 | 2.3 |
| 3
| Wills, Anaya | JR | Spruce Creek | 26.21 | FHSAA 4A Region 1 | May 1, 2021 | 1.5 |
| 4
| Jones, Aalaiah | FR | Atlantic (PO) | 26.36 | FHSAA 2A Region 2 | May 1, 2021 | 2.4 |
| 5
| Hastings, Jiya | SR | DeLand | 26.37 | Deltona Home Meet #1 | Feb 17, 2021 | |
| 6
| Means, Aniya | JR | Spruce Creek | 27.50 | FHSAA 4A District 3 | Apr 16, 2021 | 3.5 |
| 7
| LaPlante, Fabiola | FR | Flagler Palm Coast | 27.55 | FHSAA 4A District 3 | Apr 16, 2021 | 3.5 |
| 8
| Edwards, Tykerra | SO | Spruce Creek | 27.60 | FHSAA 4A District 3 | Apr 16, 2021 | 3.5 |
| 9
| LeBlanc, Shyleigh | SO | Flagler Palm Coast | 27.62 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 10 | Davis, Kylie | FR | Spruce Creek | 27.83 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 11 | Spann, Taylor | FR | Flagler Palm Coast | 28.00 | Don Hall Invitational / St. John River Conference | Apr 9, 2021 | -0.5 |
| 12 | Butler, Elise | JR | Spruce Creek | 28.10 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 13 | Barr-Miller, Nyrania | FR | University (OC) | 28.20 | University HS Titans Invitational | Apr 3, 2021 | |
| 14 | Johnson, Aleah | SR | Mainland | 28.25 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 15 | Jeffers, Ma'Shuyn | SO | Flagler Palm Coast | 28.31 | Ancient City Invitational | Mar 26, 2021 | |
| 16 | Clyde, Akeara | FR | Mainland | 28.33 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 17 | Benjamin, Anastashea | FR | Mainland | 28.35 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 18 | Barnes, Summer | FR | Flagler Palm Coast | 28.40 | Flagler Pinnacle Pre-Season Classic | Feb 12, 2021 | 0.5 |
| 19 | Stahlman, Kiley | JR | University (OC) | 28.50 | University HS Titans Invitational | Apr 3, 2021 | |
| 20 | Apakama, Jessica | SR | DeLand | 28.55 | Lake Mary Invitational 2021 | Mar 6, 2021 | 0.7 |
| 21 | Grant, Ashton | FR | Atlantic (PO) | 28.67 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 22 | Moore-Felton, Jaliyah | JR | DeLand | 28.69 | FHSAA 4A District 3 | Apr 16, 2021 | 3.2 |
| 23 | O'Reilly, Saige | SO | Seabreeze | 28.72 | FHSAA 3A District 4 | Apr 14, 2021 | -2.4 |
| 24 | Deleon, Kira | SR | Pine Ridge | 28.85 | University HS Titans Invitational | Apr 3, 2021 | |
| 25 | Martin, Briahna | SR | University (OC) | 28.93 | University HS Titans Invitational | Apr 3, 2021 | |
| 26 | Vance, Abigail | FR | Mainland | 29.10 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 27 | Sims, Madeline | SO | Flagler Palm Coast | 29.13 | FHSAA 4A District 3 | Apr 16, 2021 | 3.5 |
| 28 | Yisrael, Yashaiya | SO | Atlantic (PO) | 29.18 | East Coast Classic | Feb 26, 2021 | -0.5 |
| 29 | Williamson, Juelz | FR | DeLand | 29.25 | FHSAA 4A District 3 | Apr 16, 2021 | 3.2 |
| 30 | Livingston, Jada | FR | Atlantic (PO) | 29.38 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 31 | Jones, Makayla | FR | Atlantic (PO) | 29.39 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 32 | Van Rij, Kaylee | SR | Spruce Creek | 29.46 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 33 | Hancock, Ai | SO | DeLand | 29.47 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 34 | Gilbert, Lee-Ann | SR | Seabreeze | 29.54 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 35 | Clowers-Jones, Jayla | SO | Seabreeze | 29.62 | Spruce Creek Elite Invitational | Apr 3, 2021 | |
| 36 | Cartwright, Cheyenne | SO | DeLand | 29.63 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 37 | Olude, Asia-Marie | SR | Pine Ridge | 29.68 | University HS Titans Invitational | Apr 3, 2021 | |
| 38 | Jackson, Dashmarie | FR | Deltona | 29.78 | University HS Titans Invitational | Apr 3, 2021 | |
| 39 | Jones, Marlo | SO | University (OC) | 29.80 | University HS Titans Invitational | Apr 3, 2021 | |
| 40 | Manfredi, Christina | SR | University (OC) | 29.88 | University HS Titans Invitational | Apr 3, 2021 | |
| 41 | Yebba, Kaylee | SO | DeLand | 29.91 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 42 | Gardner, Olivia | SO | New Smyrna Beach | 30.10 | 2021 Craig Speziale Memorial Track and Field Invitational | Mar 6, 2021 | |
| 43 | Diaz , Nayla | JR | University (OC) | 30.15 | FHSAA 4A District 3 | Apr 16, 2021 | 3.2 |
| 44 | BienAime, Marlie | SR | Flagler Palm Coast | 30.23 | Bolles Mini Meet | Feb 18, 2021 | |
| 45 | Ortiz, Marlene | FR | Flagler Palm Coast | 30.24 | East Coast Classic | Feb 26, 2021 | -0.5 |
| 46 | Rieber, Ruth | JR | New Smyrna Beach | 30.25 | Spruce Creek Elite Invitational | Apr 3, 2021 | |
| 47 | Johnston, Briona | SR | Flagler Palm Coast | 30.30 | Bolles Mini Meet | Feb 18, 2021 | |
| 47 | Roberts, Alaina | FR | New Smyrna Beach | 30.30 | Tohopekaliga Invitational 1 | Feb 20, 2021 | 1.5 |
| 49 | Grier, Ashlynn | FR | Mainland | 30.40 | FHSAA 3A District 4 | Apr 14, 2021 | -2.5 |
| 50 | Flaverney, Jerianna | SO | University (OC) | 30.50 | Deltona HS - Home Meet 2 | Feb 24, 2021 | |
| 50 | Grimaldi, Ava | SO | New Smyrna Beach | 30.50 | East Coast Classic | Feb 26, 2021 | -0.7 |
| 52 | Estes, McKenna | FR | New Smyrna Beach | 30.54 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 53 | Villafane, Kayla | FR | Flagler Palm Coast | 30.59 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 54 | Walton, Lahna | FR | University (OC) | 30.68 | University HS Titans Invitational | Apr 3, 2021 | |
| 55 | Zherukha, Marta | JR | Spruce Creek | 30.77 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 56 | Braun, Alyssa | FR | Deltona | 30.82 | University HS Titans Invitational | Apr 3, 2021 | |
| 57 | Bohannon, Kenya | FR | Atlantic (PO) | 30.87 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 58 | Walker, Jalia | SO | Mainland | 30.90 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 59 | Harris, Sandra-Miriam | SO | Deltona | 31.15 | University HS Titans Invitational | Apr 3, 2021 | |
| 60 | Bryan , Shea | FR | Spruce Creek | 31.18 | Spruce Creek Time Trials | Feb 9, 2021 | |
| 61 | Power, Isabella | JR | New Smyrna Beach | 31.27 | Flagler Pinnacle Pre-Season Classic | Feb 12, 2021 | -0.3 |
| 62 | Witschey, Shelby | SR | University (OC) | 31.30 | Lake Mary Invitational 2021 | Mar 6, 2021 | -1.0 |
| 63 | McLemore, Saige | SO | New Smyrna Beach | 31.32 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 64 | Hernandez, Sophie | JR | University (OC) | 31.43 | University HS Titans Invitational | Apr 3, 2021 | |
| 65 | Taylor, Lauren | SO | Spruce Creek | 31.58 | Flagler Pinnacle Pre-Season Classic | Feb 12, 2021 | -0.3 |
| 66 | Vickers, Alexia | FR | Mainland | 31.66 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 67 | Peña, Isabella | FR | University (OC) | 31.69 | University HS Titans Invitational | Apr 3, 2021 | |
| 68 | Nash, Savannah | FR | Deltona | 31.75 | University HS Titans Invitational | Apr 3, 2021 | |
| 69 | Ealey, Michelle | SO | Mainland | 31.80 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 70 | Houston, Ava | SO | Flagler Palm Coast | 31.83 | East Coast Classic | Feb 26, 2021 | -0.5 |
| 71 | Thomas, Heaven | FR | Mainland | 31.84 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 72 | Leger, Mia | FR | Seabreeze | 31.99 | FHSAA 3A District 4 | Apr 14, 2021 | -2.5 |
| 73 | Lawrence, D'amia | SR | Mainland | 32.01 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 74 | Berry, Kyra | FR | Mainland | 32.06 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 75 | Raymond, Angela | JR | Deltona | 32.08 | University HS Titans Invitational | Apr 3, 2021 | |
| 76 | Altinor, Erika | SR | Atlantic (PO) | 32.23 | Deltona Home Meet #1 | Feb 17, 2021 | |
| 77 | Mills, Bekken | FR | Flagler Palm Coast | 32.42 | Flagler Pinnacle Pre-Season Classic | Feb 12, 2021 | -0.3 |
| 78 | Irven, Charlize | SO | Seabreeze | 32.44 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 79 | Hayes, Emani | FR | Mainland | 32.48 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 80 | Brenay, Natalie | FR | Pine Ridge | 32.49 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 81 | Wallace, Essence | JR | Atlantic (PO) | 32.64 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 82 | Martin, Aliana | FR | Seabreeze | 32.65 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 83 | Crawford, Brooklein | FR | Atlantic (PO) | 32.68 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 84 | Rivera, Aracely | SO | University (OC) | 32.73 | University HS Titans Invitational | Apr 3, 2021 | |
| 85 | Woods, Phoenix | JR | DeLand | 32.79 | East Coast Classic | Feb 26, 2021 | -0.7 |
| 86 | Bohannon, Say Naya | SO | Atlantic (PO) | 32.98 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 87 | Ford, Za'Mira | SO | Atlantic (PO) | 33.15 | Deltona Home Meet #1 | Feb 17, 2021 | |
| 88 | McKinney, Sabriah | SR | New Smyrna Beach | 33.21 | Flagler Pinnacle Pre-Season Classic | Feb 12, 2021 | -0.3 |
| 89 | Lopez, Adara | JR | University (OC) | 33.29 | University HS Titans Invitational | Apr 3, 2021 | |
| 90 | St. Louis-Bland, Soleana | SO | Flagler Palm Coast | 33.42 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 91 | Moore, Rianna | FR | Mainland | 33.55 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 92 | Fraser, Asleigh | SR | Mainland | 33.59 | Spruce Creek Developmental Meet #1 | Mar 3, 2021 | |
| 93 | Gilbert, Ava | SO | Seabreeze | 33.65 | Deltona Home Meet #1 | Feb 17, 2021 | |
| 94 | Hernandez, Anyelica | SO | University (OC) | 33.70 | East Coast Classic | Feb 26, 2021 | -1.0 |
| 95 | Rodriguez, Stephany | FR | Flagler Palm Coast | 33.90 | East Coast Classic | Feb 26, 2021 | -1.4 |
| 96 | Spanos, Alex | FR | Spruce Creek | 33.97 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
| 97 | Torres, Kayla | JR | University (OC) | 34.19 | Lake Mary Invitational 2021 | Mar 6, 2021 | -1.0 |
| 98 | Brockington, Amya | SR | Atlantic (PO) | 34.36 | Deltona Home Meet #1 | Feb 17, 2021 | |
| 99 | Evans, Deasia | FR | Atlantic (PO) | 34.38 | Spruce Creek George Janan Invitational | Mar 19, 2021 | |
| 100 | Davis, Renee | SO | Atlantic (PO) | 34.50 | Volusia Freshmen/Sophomore Meet | Mar 9, 2021 | |
