| 1
| Koon, Patrick | FR | Leon | 4:30.14 | Chiles Relays | Feb 20, 2021 |
| 2
| Rogers, Jake | SO | Leon | 4:31.28 | Chiles Relays | Feb 20, 2021 |
| 3
| Samuel, Nicholas | SR | Lincoln | 4:34.18 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 4
| Morgan, Kyle | SR | Gainesville | 4:45.77 | SFHS Mini Meet #1 | Feb 18, 2021 |
| 5
| Brownlee, Jalen | SR | Rickards | 4:47.56 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 6
| Lamar, Maxwell | SR | Leon | 4:55.77 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 7
| Kepran, Edward | SO | Arnold | 4:56.04 | Seahawks Open | Feb 20, 2021 |
| 8
| Waddell, Nicolas | FR | Arnold | 4:58.55 | Seahawks Open | Feb 20, 2021 |
| 9
| Springer, Will | FR | Leon | 4:59.03 | Chiles Relays | Feb 20, 2021 |
| 10 | Carrasquilla, Quinn | FR | Leon | 5:01.41 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 11 | Winsor, Will | FR | Leon | 5:01.77 | Chiles Relays | Feb 20, 2021 |
| 12 | Miles, Gabriel | SR | Rickards | 5:03.63 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 13 | Barnett, Nicholas | FR | Gainesville | 5:03.77 | SFHS Mini Meet #1 | Feb 18, 2021 |
| 14 | McLeod, Joseph | SO | Lincoln | 5:08.46 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 15 | Thomas, Enijah | FR | Rickards | 5:11.42 | Chiles Relays | Feb 20, 2021 |
| 16 | Smith, Grayton | FR | Lincoln | 5:12.02 | Chiles Relays | Feb 20, 2021 |
| 17 | Mliu, Yi'en | FR | Lincoln | 5:15.50 | Chiles Relays | Feb 20, 2021 |
| 18 | Callaghan, Jaden | SO | Wakulla | 5:17.27 | Wakulla Quad Meet 1 | Feb 16, 2021 |
| 19 | Schaefer, Caleb | SO | Leon | 5:18.40 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 20 | Nicholson, Elijah | SO | Lincoln | 5:19.38 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 21 | Crabtree, Liam | FR | Arnold | 5:19.88 | Arnold/Bozeman dual | Feb 27, 2021 |
| 22 | Everett, Michael | FR | Mosley | 5:21.51 | Seahawks Open | Feb 20, 2021 |
| 23 | Greenwood, Justin | FR | Rickards | 5:21.81 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 24 | Whitehead, Zakeric | SR | Leon | 5:24.79 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 25 | DelMarco-Poore, Jackson | SO | Leon | 5:25.31 | Chiles Relays | Feb 20, 2021 |
| 26 | Kilpatrick, Logan | JR | Wakulla | 5:28.14 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 27 | Monteiro, Davis | JR | Arnold | 5:28.85 | Arnold/Bozeman dual | Feb 27, 2021 |
| 28 | Culpepper, Carson | SO | Leon | 5:30.32 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 29 | McLean, Caleb | FR | Wakulla | 5:32.71 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 30 | Dickey, Davontae | SO | Rickards | 5:33.21 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 31 | Schwenkler, Jack | FR | Leon | 5:37.73 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 32 | Parsons, Ben | FR | Arnold | 5:39.16 | Arnold/Bozeman dual | Feb 27, 2021 |
| 33 | Kehayov, Martin | SO | Arnold | 5:40.63 | Arnold/Bozeman dual | Feb 27, 2021 |
| 34 | Stapleton, Cameron | SR | Wakulla | 5:41.46 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 35 | Pitts, Donnie | JR | Wakulla | 5:42.56 | Wakulla Quad Meet 1 | Feb 16, 2021 |
| 36 | Darvai, Kristof | SO | Mosley | 5:44.25 | Seahawks Open | Feb 20, 2021 |
| 37 | Gibson, Brantley | SO | Rickards | 5:44.82 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 38 | McGinnis, Malcolm | JR | Lincoln | 5:46.50 | Chiles Relays | Feb 20, 2021 |
| 39 | Tatum, Christian | SO | Arnold | 5:47.47 | Seahawks Open | Feb 20, 2021 |
| 40 | McClendon, Joshua | FR | Rickards | 5:47.91 | Chiles Relays | Feb 20, 2021 |
| 41 | Wiedeman, Joseph | SO | Wakulla | 5:51.29 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 42 | Blue, Kelton | JR | Arnold | 5:52.26 | Arnold/Bozeman dual | Feb 27, 2021 |
| 43 | McNeal, Donovan | SR | Lincoln | 5:53.07 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 44 | Bentley, Joshua | SO | Wakulla | 5:54.65 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 45 | Mayer, Rodderick | FR | Lincoln | 5:56.02 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 46 | Baker, Evan | FR | Gainesville | 5:57.32 | SFHS Mini Meet #1 | Feb 18, 2021 |
| 47 | Cottrell, Tristan | FR | Lincoln | 5:58.40 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 48 | Swope, Isaac | SR | Wakulla | 6:02.65 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 49 | Isom, Kristian | SO | Mosley | 6:10.80 | Seahawks Open | Feb 20, 2021 |
| 50 | Wright, Olatunde | FR | Lincoln | 6:14.17 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 51 | Langston, Jacob | FR | Wakulla | 6:17.88 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 52 | Cully, David | FR | Wakulla | 6:20.00 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 53 | Peck, Isaac | SO | Wakulla | 6:22.58 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 54 | Taylor, Myles | JR | Mosley | 6:26.27 | Seahawks Open | Feb 20, 2021 |
| 55 | Avello, Isaac | FR | Wakulla | 6:33.57 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 56 | Marshall, Alex | FR | Wakulla | 6:38.32 | Wakulla Quad Meet 2 | Feb 25, 2021 |
| 57 | Wrighton, Jonathan | SO | Gainesville | 6:40.68 | SFHS Mini Meet #1 | Feb 18, 2021 |
| 58 | Knight, Ethan | FR | Lincoln | 6:42.55 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 59 | Pesate, Nathan | SR | Arnold | 6:51.51 | Arnold/Bozeman dual | Feb 27, 2021 |
| 60 | Capps, Zach | SR | Arnold | 6:55.02 | Arnold/Bozeman dual | Feb 27, 2021 |
| 61 | Allen, Julian | FR | Lincoln | 6:56.47 | Jesse Forbes HS Invitational | Feb 27, 2021 |
| 62 | Peebles, Braden | FR | Wakulla | 6:59.72 | Wakulla Quad Meet 2 | Feb 25, 2021 |
