1 | Denton, TySheem | SR | North Marion | 10.95 | FHSAA 2A Region 2 | May 1, 2021 | 3.9 |
| 2 | Isaac, Alvon | FR | Villages | 11.04 | FHSAA 2A Region 2 | May 1, 2021 | 3.9 |
| 3 | Foster, Chris | SO | North Marion | 11.06 | FHSAA 2A Region 2 | May 1, 2021 | 2.7 |
| 4 | Gomez, Leonardo | SR | Dunnellon | 11.13 | FHSAA 2A Region 2 | May 1, 2021 | 2.7 |
| 5
| Harris, David | SR | Mount Dora | 11.31 | Mount Dora Christian Invitational Meet #4 | Apr 3, 2021 | |
| 6
| Mccoy , Bayfus | JR | Eustis | 11.36 | The Villages Charter High School Meet Two | Mar 11, 2021 | |
| 7
| Shipp, Brenden | SR | Villages | 11.37 | FHSAA 2A District 6 | Apr 22, 2021 | |
| 8
| Diggs, Kobe | JR | Umatilla | 11.40 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 9
| McNair, Jamel | SR | North Marion | 11.46 | Wildwood High School Invitational | Feb 27, 2021 | |
| 10 | Griffin, Ja'lan | JR | Umatilla | 11.49 | The Villages Charter High School Meet Two | Mar 11, 2021 | |
| 11 | Abate, Daijhae | SR | Trinity Catholic | 11.55 | Trinity Catholic Invitational | Mar 13, 2021 | 0.2 |
| 12 | Anderson, Jaden | JR | Eustis | 11.59 | The Villages Charter High School Meet Two | Mar 11, 2021 | |
| 13 | McCants, Demetrius | JR | Dunnellon | 11.67 | MCIAC Championships | Mar 26, 2021 | |
| 14 | Morgan, Jack | JR | Mount Dora | 11.74 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 15 | Fashaw, Christiaan | SR | Dunnellon | 11.79 | MCIAC Championships | Mar 26, 2021 | |
| 16 | Rogers, Toren | JR | Mount Dora | 11.80 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 17 | Scott, Andrew | JR | Mount Dora | 11.81 | The Villages Buffalo Invitational | Mar 27, 2021 | |
| 18 | Hayes, John | FR | Trinity Catholic | 11.83 | Trinity Catholic Invitational | Mar 13, 2021 | -0.6 |
| 19 | Snowden, Terrell | SR | Trinity Catholic | 11.85 | Trinity Catholic Last Chance Meet | Apr 10, 2021 | |
| 20 | Johnson-Turner, Dante | SO | Mount Dora | 11.92 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 21 | Williams, Edward | JR | Villages | 11.95 | South Sumter High School Fool's Day Open | Apr 1, 2021 | |
| 22 | Allen, Chris | FR | Trinity Catholic | 11.99 | Trinity Catholic Invitational | Mar 13, 2021 | -0.6 |
| 22 | Hollis Jr. , Tinnibu | SR | Dunnellon | 11.99 | MCIAC Championships | Mar 26, 2021 | |
| 22 | Walker, Caleb | FR | Trinity Catholic | 11.99 | Trinity Catholic Classic | Feb 20, 2021 | 1.7 |
| 25 | Senner, Riley | JR | Mount Dora | 12.03 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 26 | Williams, Devin | SO | Tavares | 12.04 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 27 | williams, jharez | FR | Williston | 12.14 | FHSAA 2A District 6 | Apr 22, 2021 | |
| 28 | Clouthier, Benjamin | SO | Mount Dora | 12.15 | The Villages Charter High School Meet Two | Mar 11, 2021 | |
| 29 | Cody, De'Andre | SO | Eustis | 12.16 | The Villages Charter High School Meet Two | Mar 11, 2021 | |
| 29 | Mestre, Alijah | FR | North Marion | 12.16 | Wildwood High School Invitational | Feb 27, 2021 | |
| 31 | Ficket, Russell | FR | Mount Dora | 12.21 | The Villages Buffalo Invitational | Mar 27, 2021 | |
| 32 | Hawkins, Logan | SR | Eustis | 12.23 | The Villages Charter High School Meet Two | Mar 11, 2021 | |
| 33 | Evans, Ja'Quim | SR | North Marion | 12.28 | Wildwood High School Invitational | Feb 27, 2021 | |
| 34 | Benoit, Adrian | JR | Tavares | 12.38 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 35 | Garcia, Serguey | SO | North Marion | 12.41 | FHSAA 2A District 6 | Apr 22, 2021 | |
| 36 | Sayles, Isaiah | JR | Eustis | 12.47 | Mount Dora Christian Developmental Meet #3 | Feb 25, 2021 | |
| 37 | Williams, Lashaun | FR | Eustis | 12.48 | Zephyrhills High Quad Meet | Mar 23, 2021 | |
| 38 | Keeling, Eli | SR | Tavares | 12.49 | Mount Dora Christian Invitational Meet #4 | Apr 3, 2021 | |
| 39 | Craft, Andres | SO | Eustis | 12.50 | FHSAA 2A District 6 | Apr 22, 2021 | |
| 39 | Deason, Denzel | SR | North Marion | 12.50 | Trinity Catholic Classic | Feb 20, 2021 | 2.9 |
| 41 | Beckett, Brandon | SR | North Marion | 12.53 | Trinity Catholic Tuesday Invitational | Apr 6, 2021 | -0.1 |
| 42 | Webb, Zayshawn | FR | North Marion | 12.56 | Trinity Catholic Classic | Feb 20, 2021 | 0.5 |
| 43 | Riveria, Isaiah | SO | Umatilla | 12.63 | FHSAA 2A District 6 | Apr 22, 2021 | |
| 44 | Arias, Jason | JR | North Marion | 12.67 | Trinity Catholic Classic | Feb 20, 2021 | 2.4 |
| 44 | Reynonce, Queny | JR | Eustis | 12.67 | Zephyrhills High Quad Meet | Mar 23, 2021 | |
| 46 | Goldwire, Corey | SR | Villages | 12.70 | The Villages Charter High School Meet Two | Mar 11, 2021 | |
| 47 | Ackerman, Devin | SR | Tavares | 12.76 | FHSAA 2A District 6 | Apr 22, 2021 | |
| 48 | Sanchez, Steven | SO | North Marion | 12.86 | The Villages Buffalo Invitational | Mar 27, 2021 | |
| 49 | Derosa, Jaiden | JR | North Marion | 12.87 | Trinity Catholic Invitational | Mar 13, 2021 | -0.6 |
| 50 | DuRant, Ronnie | JR | Mount Dora | 13.00 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 51 | Sarakinis, Kristian | FR | Villages | 13.04 | The Villages Buffalo Invitational | Mar 27, 2021 | |
| 52 | Lluberes, Jewellius | FR | Trinity Catholic | 13.06 | Trinity Catholic Tuesday Classic | Mar 23, 2021 | -2.3 |
| 53 | Deregnaucourt, Mason | SO | Villages | 13.37 | Wildwood High School Invitational | Feb 27, 2021 | |
| 54 | Eden, Aiden | JR | Williston | 13.38 | Chiefland Home Meet 3 | Mar 16, 2021 | |
| 55 | Dawkins, Haiden | FR | Tavares | 13.46 | FHSAA 2A District 6 | Apr 22, 2021 | |
| 55 | Switzer, Sullivan | FR | North Marion | 13.46 | MCIAC Championships | Mar 26, 2021 | |
| 57 | Seal, Trey | FR | Mount Dora | 13.54 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 58 | Mooloo, Vijay | JR | Tavares | 13.81 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 59 | Torres-Lopez, Fenando | JR | Villages | 14.02 | Wildwood High School Invitational | Feb 27, 2021 | |
| 60 | Wilson, Kason | FR | Umatilla | 14.14 | Mount Dora Christian Developmental Meet #3 | Feb 25, 2021 | |
| 61 | Almanza, Eddie | 6
| Williston | 17.27 | SFHS Mini Meet #2 | Mar 9, 2021 | |
FHSAA 2A