1 | Lopez, Darion | SR | Pensacola Catholic | 49.77 | FHSAA Class 2A/Para-Athlete Track & Field State Championship | May 8, 2021 |
| 2 | Moore, Hayden | SR | South Walton | 50.92 | Tommy Taylor High School Invitational | Mar 13, 2021 |
| 3 | Provau, Ryan | FR | West Florida | 52.22 | Tate Aggies Invitational | Apr 3, 2021 |
| 4
| Chavers, Ethan | SO | Pensacola Catholic | 53.06 | FHSAA 2A District 1 | Apr 15, 2021 |
| 5
| Campbell, Charles | SR | Bay | 53.17 | Bozeman Invitational Meet | Mar 11, 2021 |
| 6
| Summerlin, Evan | SO | South Walton | 53.29 | FHSAA 2A District 1 | Apr 15, 2021 |
| 7
| Reed, Jabari | SO | Bay | 53.36 | FHSAA 2A District 1 | Apr 15, 2021 |
| 8
| Layne, Adam | JR | Bozeman | 53.96 | Bozeman Invitational Meet | Mar 11, 2021 |
| 9
| Stallworth, Lawrence | JR | Pensacola | 54.43 | Pensacola Tiger Invitational | Mar 13, 2021 |
| 10 | Lamon, Conner | JR | South Walton | 55.19 | FHSAA 2A District 1 | Apr 15, 2021 |
| 11 | Bell, Dredrick | JR | Pensacola | 55.32 | Pensacola Tiger Invitational | Mar 13, 2021 |
| 12 | Paul, Kaileb | SR | Bay | 55.47 | Bay County Track & Field Championships | Apr 8, 2021 |
| 13 | Hunter, Paul | SR | Rutherford | 55.54 | FHSAA 2A District 1 | Apr 15, 2021 |
| 14 | Marcelonis, Dolan | SR | Pensacola Catholic | 55.71 | FHSAA 2A District 1 | Apr 15, 2021 |
| 15 | Thepchantee, Levi | SO | Walton | 55.96 | FHSAA 2A District 1 | Apr 15, 2021 |
| 16 | Weaver, Anthoni | JR | Northview | 56.07 | Jay QB Invitational | Apr 6, 2021 |
| 17 | Watson, Nolan | SO | South Walton | 56.23 | Seahawks Open | Feb 20, 2021 |
| 18 | McWilliams, Jeremiah | JR | Pensacola | 56.29 | Andrews Invitational 2021 | Mar 26, 2021 |
| 19 | Hutcherson, Kenneth | SO | Bay | 56.90 | Marlin (Arnold) Invite | Mar 25, 2021 |
| 20 | Salter, Drake | SO | Pensacola Catholic | 57.03 | Pensacola Tiger Invitational | Mar 13, 2021 |
| 21 | Jackson, Theodore | SO | Pensacola | 57.30 | Tate Aggies Invitational | Apr 3, 2021 |
| 22 | Faint, Michael | 8
| North Bay Haven | 57.64 | Bozeman Invitational Meet | Mar 11, 2021 |
| 23 | Ray, Steven | SR | North Bay Haven | 57.79 | Bozeman Host Meet | Apr 1, 2021 |
| 24 | Sims, Kimon | SO | Pensacola | 57.93 | Pensacola Tiger Invitational | Mar 13, 2021 |
| 25 | Sapp, Eric | JR | Bozeman | 58.00 | Bay County Track & Field Championships | Apr 8, 2021 |
| 26 | King, Jalen | SR | West Florida | 58.29 | Steve Garst Wildcat Invitational | Mar 6, 2021 |
| 27 | Holmes, Roman | 8
| Rutherford | 58.31 | Bozeman Host Meet | Apr 1, 2021 |
| 28 | Timmerman, Wyatt | JR | Rutherford | 58.52 | Bozeman Invitational Meet | Mar 11, 2021 |
| 29 | Black, Romero | SO | Rutherford | 58.58 | FHSAA 2A District 1 | Apr 15, 2021 |
| 30 | Thomas, Tywann | SR | Pensacola | 58.65 | Tate Aggies Invitational | Apr 3, 2021 |
| 31 | Cozart, Seth | SR | North Bay Haven | 58.78 | Bozeman Invitational Meet | Mar 11, 2021 |
| 32 | Walker, Shawn | FR | North Bay Haven | 59.06 | Bay County Track & Field Championships | Apr 8, 2021 |
| 33 | Dortch, Steven | SR | Pensacola | 59.12 | Pensacola Tiger Invitational | Mar 13, 2021 |
| 34 | Kilburn, Ethan | JR | Northview | 59.67 | Tate Aggies Invitational | Apr 3, 2021 |
| 35 | Hughes, Jesse | JR | Northview | 59.78 | Tate Aggies Invitational | Apr 3, 2021 |
| 36 | Bodiker, Garrett | SR | Northview | 1:00.26 | Andrews Invitational 2021 | Mar 26, 2021 |
| 37 | Baker, Brayden | 8
| Bozeman | 1:00.33 | FHSAA 2A District 1 | Apr 15, 2021 |
| 38 | O'Rourke, Thailon | SO | Bay | 1:00.59 | Seahawks Open | Feb 20, 2021 |
| 39 | Jordan, Zane | FR | South Walton | 1:00.70 | FHSAA 2A District 1 | Apr 15, 2021 |
| 40 | boatwright, daniel | FR | Walton | 1:00.92 | Jay QB Invitational | Apr 6, 2021 |
| 41 | Lucas, Kobi | SR | Bozeman | 1:00.96 | Marlin (Arnold) Invite | Mar 25, 2021 |
| 42 | Ganske, Kiernan | FR | South Walton | 1:00.98 | Seahawks Open | Feb 20, 2021 |
| 43 | Sanchez, Sebastian | SR | Bay | 1:01.09 | Bozeman Invitational Meet | Mar 11, 2021 |
| 44 | Ellis, Amante | SO | Rutherford | 1:01.24 | Seahawks Open | Feb 20, 2021 |
| 45 | Rumph, D'elton | SR | Bay | 1:01.45 | Seahawks Invitational | Mar 6, 2021 |
| 46 | Clemmons, Cameron | SO | Pensacola | 1:01.47 | FHSAA 2A District 1 | Apr 15, 2021 |
| 47 | Dawsey, Xavier | FR | Walton | 1:01.54 | FHSAA 2A District 1 | Apr 15, 2021 |
| 48 | Carinhas, Thomas | SO | North Bay Haven | 1:01.79 | Seahawks Invitational | Mar 6, 2021 |
| 49 | Conerly, Jayshawn | SO | Bay | 1:01.81 | Bozeman Invitational Meet | Mar 11, 2021 |
| 50 | Dalbey, Noah | SO | South Walton | 1:02.24 | Chiles Capital City Classic | Apr 3, 2021 |
| 51 | Bagwell, Skylar | SO | Bozeman | 1:02.47 | FHSAA 2A District 1 | Apr 15, 2021 |
| 52 | Talbot, David | JR | Bozeman | 1:02.73 | Bozeman Invitational Meet | Mar 11, 2021 |
| 53 | Fields, Caleb | SR | Bay | 1:02.95 | Bozeman Invitational Meet | Mar 11, 2021 |
| 54 | Carstarphen, Billy | SR | Bay | 1:03.11 | Seahawks Open | Feb 20, 2021 |
| 55 | Houston, Sam | JR | North Bay Haven | 1:03.29 | Bozeman Invitational Meet | Mar 11, 2021 |
| 56 | Kowalik, Brett | FR | Bozeman | 1:03.77 | Bozeman Invitational Meet | Mar 11, 2021 |
| 56 | Owens, Sebastian | FR | North Bay Haven | 1:03.77 | Bozeman Invitational Meet | Mar 11, 2021 |
| 58 | gillert, austin | FR | Walton | 1:05.16 | Bozeman Host Meet | Apr 1, 2021 |
| 59 | Moore, Jaidev | JR | Bay | 1:05.92 | Bozeman Invitational Meet | Mar 11, 2021 |
| 60 | Jamison, Logan | 7
| Bozeman | 1:06.27 | Bozeman Host Meet | Apr 1, 2021 |
| 61 | johnston, joshua | FR | Walton | 1:06.98 | Baker Home Meet 2 | Mar 11, 2021 |
| 62 | Holmes, Hunter | JR | Walton | 1:07.68 | Baker Home Meet 2 | Mar 11, 2021 |
| 63 | Watford, Joseph | JR | Rutherford | 1:08.91 | Seahawks Invitational | Mar 6, 2021 |
| 64 | Martinez, Louis | 7
| Bozeman | 1:09.06 | Bozeman Invitational Meet | Mar 11, 2021 |
| 65 | Carroll, Nathan | FR | Pensacola Catholic | 1:09.55 | Jay Home Meet 1 | Mar 9, 2021 |
| 66 | Rackard, Dallon | JR | Northview | 1:10.72 | Steve Garst Wildcat Invitational | Mar 6, 2021 |
| 67 | Tillman, Finn | FR | Pensacola Catholic | 1:12.70 | Pine Forest Invitational | Feb 20, 2021 |
| 68 | Ruthven, Conner | 7
| Bozeman | 1:15.97 | Bozeman Invitational Meet | Mar 11, 2021 |
| 69 | Starke, Tobi | FR | Bay | 1:21.83 | Bozeman Invitational Meet | Mar 11, 2021 |
FHSAA 2A