1 | Mikell, Oscene | SR | Wildwood | 22.42 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 2 | Bennett, Dinari | JR | First Academy (Orlando) | 22.50 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 3 | Smith, Omari | JR | Wildwood | 22.72 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 4 | Colebrooks, Jeremiah | JR | Wildwood | 23.10 | FHSAA 1A Region 2 | May 1, 2021 | -0.7 |
| 5 | Richards, Will | JR | First Academy (Orlando) | 23.35 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 6
| Robinson, Jaylan | SR | First Academy (Orlando) | 23.72 | Kowboy Invitational | Mar 12, 2021 | 1.7 |
| 7
| Johnson, Sean | SR | First Academy (Orlando) | 23.87 | Saints Invitational 2021 | Apr 7, 2021 | -1.8 |
| 8
| McArdle, Tyler | SR | First Academy (Orlando) | 23.99 | Mount Dora Christian Invitational Meet #3 | Mar 27, 2021 | |
| 9
| Brookins, Vincent | SR | Foundation Academy | 24.04 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 10 | Clements, Cashe | FR | Wildwood | 24.06 | Wildwood High School Invitational | Feb 27, 2021 | |
| 11 | McManus, Carson | FR | Circle Christian | 24.09 | Mount Dora Christian Invitational Meet #2 | Mar 20, 2021 | |
| 12 | Belflower, Joseph | JR | Orangewood Christian | 24.29 | Bob Mosher Wildcat Open/Lady Wildcat Open | Apr 3, 2021 | 0.4 |
| 13 | Pearson, Jackson | JR | Circle Christian | 24.41 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 14 | Susla, Rylan | SO | Orangewood Christian | 24.53 | Mount Dora Christian Invitational Meet #3 | Mar 27, 2021 | |
| 15 | Jean, Daniel | FR | Foundation Academy | 24.65 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 16 | Yepes, Sebastian | SO | First Academy (Orlando) | 24.67 | Saints Invitational 2021 | Apr 7, 2021 | -0.8 |
| 17 | Jackson, Nick | JR | First Academy (Leesburg) | 24.68 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 18 | Andral-Colon, Jaden | SR | Foundation Academy | 24.77 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 19 | Parrish, Beau | FR | First Academy (Leesburg) | 25.13 | Wildwood High School Invitational | Feb 27, 2021 | |
| 20 | Brown, Alex | SR | Wildwood | 25.34 | The Villages Charter High School Meet One | Feb 15, 2021 | |
| 21 | Edmonds, Tyson | JR | Mount Dora Christian | 25.46 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 22 | Strickland, Jessie | JR | Wildwood | 25.53 | The Villages Charter High School Meet One | Feb 15, 2021 | |
| 23 | Goins, Johnny | SR | Wildwood | 25.57 | The Villages Charter High School Meet One | Feb 15, 2021 | |
| 24 | Jacobs, Jett | JR | Orangewood Christian | 25.61 | Mount Dora Christian Developmental Meet #4 | Mar 4, 2021 | |
| 25 | Hollowell, Chase | JR | First Academy (Leesburg) | 25.67 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 26 | Cota, Paul | SO | Circle Christian | 25.95 | University HS Titans Invitational | Apr 3, 2021 | |
| 27 | Tenczar, Jason | SO | First Academy (Orlando) | 25.96 | Royal Invitational @ TFA 2021 | Mar 5, 2021 | 0.3 |
| 28 | Keiler, Tyrese | 8
| Wildwood | 26.00 | Wildwood High School Early Season Open | Feb 20, 2021 | |
| 29 | Beloni, Caleb | 8
| Foundation Academy | 26.14 | Mount Dora Christian Invitational Meet #3 | Mar 27, 2021 | |
| 30 | Vergare, Evan | JR | Circle Christian | 26.24 | Satellite High Reggie Johnson Invite 2021 | Feb 26, 2021 | |
| 31 | Brown, Yosef | 8
| Central Florida Christian | 26.41 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 32 | Wagenknecht, Christian | SO | First Academy (Orlando) | 26.55 | Mount Dora Christian Invitational Meet #3 | Mar 27, 2021 | |
| 33 | Robinson, Joshua | FR | First Academy (Orlando) | 26.69 | Royal Invitational @ TFA 2021 | Mar 5, 2021 | 0.8 |
| 34 | Kloznick, Bray | JR | Circle Christian | 26.71 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 35 | Koziara, Michael | FR | First Academy (Orlando) | 26.79 | Highlander Classic @ LHP 2021 | Mar 12, 2021 | 1.3 |
| 36 | Mason, Bradley | FR | First Academy (Orlando) | 26.85 | Saints Invitational 2021 | Apr 7, 2021 | -0.8 |
| 37 | Bulger, Elijah | FR | Orangewood Christian | 27.39 | Mount Dora Christian Invitational Meet #3 | Mar 27, 2021 | |
| 38 | Posado, Justin | FR | Mount Dora Christian | 27.67 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 39 | Mellen, Zachary | SO | Orangewood Christian | 28.08 | Mount Dora Christian Invitational Meet #5 | Apr 9, 2021 | |
| 40 | Rey, Sebastian | FR | Foundation Academy | 28.22 | Mount Dora Christian Invitational Meet #3 | Mar 27, 2021 | |
| 41 | Diaz, Josue | SO | Central Florida Christian | 28.31 | Mount Dora Christian Invitational Meet #1 | Mar 12, 2021 | |
| 42 | Ferraz, Daniel | 8
| Foundation Academy | 28.76 | Mount Dora Christian Developmental Meet #4 | Mar 4, 2021 | |
| 43 | Padial, Diego | FR | Mount Dora Christian | 29.03 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 44 | Clements, Joel | FR | Foundation Academy | 29.31 | 46th Montverde Eagle Invitational 2021 | Feb 26, 2021 | -0.7 |
| 45 | Oldham, Ryan | FR | Mount Dora Christian | 31.30 | Mount Dora Christian Developmental Meet #4 | Mar 4, 2021 | |
| 46 | Boudreau, Logan | FR | Mount Dora Christian | 31.50 | FHSAA 1A District 7 | Apr 16, 2021 | |
| 47 | Medina, Diego | FR | Mount Dora Christian | 31.63 | Mount Dora Christian Developmental Meet #4 | Mar 4, 2021 | |
FHSAA 1A